LOCUS       sequence-293822-        6947 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human SUZ12 in insect cells, under a TEV-cleavable 
            N-terminal polyhistidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6947)
  AUTHORS   Zhang Q, McKenzie NJ, Warneford-Thomson R, Gail EH, Flanigan SF, 
            Owen BM, Lauman R, Levina V, Garcia BA, Schittenhelm RB, Bonasio R, 
            Davidovich C
  TITLE     RNA exploits an exposed regulatory site to inhibit the enzymatic 
            activity of PRC2.
  JOURNAL   Nat Struct Mol Biol. 2019 Mar;26(3):237-247. doi: 
            10.1038/s41594-019-0197-y. Epub 2019 Mar 4.
  PUBMED    30833789
REFERENCE   2  (bases 1 to 6947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-019-0197-y"; journalName: "Nat Struct Mol Biol"; 
            date: "2019-03"; volume: "26"; issue: "3"; pages: "237-247"
COMMENT     Sequence Label: pFBOH-MHL.SUZ12
FEATURES             Location/Qualifiers
     source          1..6947
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        183..274
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     199..216
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             333..350
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             363..383
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    2735..2869
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2772..2791)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2826..2845
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  2898..3063
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      3247..3701
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3334..3353)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3543..3564
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3727..3831
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3832..3900,3901..4692)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4050..4069)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4863..5451
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5352..5371
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5605..5622
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  5756..5980
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(6047..6580)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(6750..6755,6756..6772,6773..6778))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 acggatctgc cctggcttca ggagatcgga agacctcggc cgtcgcggcg cttgccggtg
       61 gtgctgaccc cggatgaagt ggttcgcatc ctcggttttc tggaaggcga gcatcgtttg
      121 ttcgcccagg actctagcta tagttctagt ggttggctac gtatactccg gaatattaat
      181 agatcatgga gataattaaa atgataacca tctcgcaaat aaataagtat tttactgttt
      241 tcgtaacagt tttgtaataa aaaaacctat aaatattccg gattattcat accgtcccac
      301 catcgggcgc ggatctcggt ccgaaaacca tgcatcatca tcatcatcac agcagcggca
      361 gagaaaactt gtatttccag ggcgcgcctc agaagcacgg cggtggggga gggggcggct
      421 cggggcccag cgcggggtcc gggggaggcg gcttcggggg ttcggcggcg gtggcggcgg
      481 cgacggcttc gggcggcaaa tccggcggcg ggagctgtgg agggggtggc agttactcgg
      541 cctcctcctc ctcctccgcg gcggcagcgg cgggggctgc ggtgttaccg gtgaagaagc
      601 cgaaaatgga gcacgtccag gctgaccacg agcttttcct ccaggccttt gagaagccaa
      661 cacagatcta tagatttctt cgaactcgga atctcatagc accaatattt ttgcacagaa
      721 ctcttactta catgtctcat cgaaactcca gaacaaacat caaaaggaaa acatttaaag
      781 ttgatgatat gttatcaaaa gtagagaaaa tgaaaggaga gcaagaatct catagcttgt
      841 cagctcattt gcagcttacg tttactggtt tcttccacaa aaatgataag ccatcaccaa
      901 actcagaaaa tgaacaaaat tctgttaccc tggaagtcct gcttgtgaaa gtttgccaca
      961 aaaaaagaaa ggatgtaagt tgtccaataa ggcaagttcc cacaggtaaa aagcaggtgc
     1021 ctttgaatcc tgacctcaat caaacaaaac ccggaaattt cccgtccctt gcagtttcca
     1081 gtaatgaatt tgaacctagt aacagccata tggtgaagtc ttactcgttg ctatttagag
     1141 tgactcgtcc aggaagaaga gagtttaatg gaatgattaa tggagaaacc aatgaaaata
     1201 ttgatgtcaa tgaagagctt ccagccagaa gaaaacgaaa tcgtgaggat ggggaaaaga
     1261 catttgttgc acaaatgaca gtatttgata aaaacaggcg cttacagctt ttagatgggg
     1321 aatatgaagt agccatgcag gaaatggaag aatgtccaat aagcaagaaa agagcaacat
     1381 gggagactat tcttgatggg aagaggctgc ctccattcga aacattttct cagggaccta
     1441 cgttgcagtt cactcttcgt tggacaggag agacgaatga taaatctacg gctcctattg
     1501 ccaaacctct tgccactaga aattcagaga gtctccatca ggaaaacaag cctggttcag
     1561 ttaaacctac tcaaactatt gctgttaaag aatcattgac tacagatcta caaacaagaa
     1621 aagaaaagga tactccaaat gaaaaccgac aaaaattaag aatattttat cagtttctct
     1681 ataacaacaa tacaaggcaa caaactgaag caagagatga cctgcattgc ccttggtgta
     1741 ctctgaactg ccgcaaactt tatagtttac tcaagcatct taaactctgc catagcagat
     1801 ttatcttcaa ctatgtttat catccaaaag gtgctaggat agatgtttct atcaatgagt
     1861 gttatgatgg ctcctatgca ggaaatcctc aggatattca tcgccaacct ggatttgctt
     1921 ttagtcgcaa cggaccagtt aagagaacac ctatcacaca tattcttgtg tgcaggccaa
     1981 aacgaacaaa agcaagcatg tctgaatttc ttgaatctga agatggggaa gtagaacagc
     2041 aaagaacata tagtagtggc cacaatcgtc tgtatttcca tagtgatacc tgcttacctc
     2101 tccgtccaca agaaatggaa gtagatagtg aagatgaaaa ggatcctgaa tggctaagag
     2161 aaaaaaccat tacacaaatt gaagagtttt ctgatgttaa tgaaggagag aaagaagtga
     2221 tgaaactctg gaatctccat gtcatgaagc atgggtttat tgctgacaat caaatgaatc
     2281 atgcctgtat gctgtttgta gaaaattatg gacagaaaat aattaagaag aatttatgtc
     2341 gaaacttcat gcttcatcta gtcagcatgc atgactttaa tcttattagc ataatgtcaa
     2401 tagataaagc tgttaccaag ctccgtgaaa tgcagcaaaa attagaaaag ggggaatctg
     2461 cttcccctgc aaacgaagaa ataactgaag aacaaaatgg gacagcaaat ggatttagtg
     2521 aaattaactc aaaagagaaa gctttggaaa cagatagtgt ctcaggggtt tcaaaacaga
     2581 gcaaaaaaca aaaactctga tgacgaagct tgtcgagaag tactagagga tcataatcag
     2641 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     2701 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     2761 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     2821 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatct gatcactgat
     2881 atcgcctagg agatccgaac cagataagtg aaatctagtt ccaaactatt ttgtcatttt
     2941 taattttcgt attagcttac gacgctacac ccagttccca tctattttgt cactcttccc
     3001 taaataatcc ttaaaaactc catttccacc cctcccagtt cccaactatt ttgtccgccc
     3061 acagcggggc atttttcttc ctgttatgtt tttaatcaaa catcctgcca actccatgtg
     3121 acaaaccgtc atcttcggct actttttctc tgtcacagaa tgaaaatttt tctgtcatct
     3181 cttcgttatt aatgtttgta attgactgaa tatcaacgct tatttgcagc ctgaatggcg
     3241 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     3301 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     3361 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     3421 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     3481 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttaa
     3541 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     3601 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     3661 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
     3721 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     3781 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     3841 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     3901 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     3961 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     4021 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     4081 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     4141 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     4201 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     4261 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     4321 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     4381 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     4441 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     4501 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     4561 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     4621 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     4681 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     4741 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     4801 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     4861 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     4921 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     4981 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     5041 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     5101 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     5161 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     5221 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     5281 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     5341 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     5401 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     5461 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     5521 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     5581 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
     5641 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcataga ccagccgcgt
     5701 aacctggcaa aatcggttac ggttgagtaa taaatggatg ccctgcgtaa gcgggtgtgg
     5761 gcggacaata aagtcttaaa ctgaacaaaa tagatctaaa ctatgacaat aaagtcttaa
     5821 actagacaga atagttgtaa actgaaatca gtccagttat gctgtgaaaa agcatactgg
     5881 acttttgtta tggctaaagc aaactcttca ttttctgaag tgcaaattgc ccgtcgtatt
     5941 aaagaggggc gtggccaagg gcatggtaaa gactatattc gcggcgttgt gacaatttac
     6001 cgaacaactc cgcggccggg aagccgatct cggcttgaac gaattgttag gtggcggtac
     6061 ttgggtcgat atcaaagtgc atcacttctt cccgtatgcc caactttgta tagagagcca
     6121 ctgcgggatc gtcaccgtaa tctgcttgca cgtagatcac ataagcacca agcgcgttgg
     6181 cctcatgctt gagcagattg atgagcgcgg tggcaatgcc ctgcctccgg tgctcgccgg
     6241 agactgcgag atcatagata tagatctcac tacgcggctg ctcaaacttg ggcagaacgt
     6301 aagccgcgag agcgccaaca accgcttctt ggtcgaaggc agcaagcgcg atgaatgtct
     6361 tactacggag caagttcccg aggtaatcgg agtccggctg atgttgggag taggtggcta
     6421 cgtctccgaa ctcacgaccg aaaagatcaa gagcagcccg catggatttg acttggtcag
     6481 ggccgagcct acatgtgcga atgatgccca tacttgagcc acctaacttt gttttagggc
     6541 gactgccctg ctgcgtaaca tcgttgctgc tccataacat caaacatcga cccacggcgt
     6601 aacgcgcttg ctgcttggat gcccgaggca tagactgtac aaaaaaacag tcataacaag
     6661 ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt
     6721 tgcgtgagcg catacgctac ttgcattaca gtttacgaac cgaacaggct tatgtcaact
     6781 gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc ggcaaccttg ggcagcagcg
     6841 aagtcgaggc atttctgtcc tggctggcga acgagcgcaa ggtttcggtc tccacgcatc
     6901 gtcaggcatt ggcggccttg ctgttcttct acggcaaggt gctgtgc
//
