LOCUS       sequence-29410-e         650 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-KHK
FEATURES             Location/Qualifiers
     source          1..650
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    72..96
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             507..518
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat gagcaatgct
       61 tttttataat gccaactttg tacaaaaaag ttggcatgga agagaagcag atcctgtgcg
      121 tggggctagt ggtgctggac gtcatcagcc tggtggacaa gtaccctaag gaggactcgg
      181 agataaggtg tttgtcccag agatggcagc gcggaggcaa cgcgtccaac tcctgcacca
      241 ttctctccct gctcggagcc ccctgtgcct tcatgggctc aatggctcct ggccatgttg
      301 ctgattttgt cctggatgac ctccgccgtt attctgtgga cctacgctac acagtctttc
      361 agaccacagg ctccgtcccc atcgccacgg tcatcatcaa cgaggccagt ggtagccgca
      421 ccatcctata ctatgacagg agcctgccag atgtgtctgc tacagacttt gagaaggttg
      481 atctgaccca gttcaagtgg atccacattg agggccggaa cgcatcggag caggtgaaga
      541 tgctgcagcg gatagacgca cacaacacca ggcagcctcc agagcagaag atccgggtgt
      601 ccgtggaggt ggagaagcca cgagaggagc tcttccagct gtttggctac
//
