LOCUS       sequence-29420-e         706 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-AK1
FEATURES             Location/Qualifiers
     source          1..706
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    7..106
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      341..350
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tcgggcccaa taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat
       61 gagcaatgct tttttataat gccaactttg tacaaaaaag ttggcatgga agagaagctg
      121 aagaaaacca atatcatctt tgtggtgggt gggcctggct cagggaaggg cacccagtgt
      181 gagaagatcg tgcagaagta tggctacacc cacctctcca ccggggacct cctgcggtcc
      241 gaggtcagct caggctcggc caggggcaag aagctgtcgg aaatcatgga gaaggggcag
      301 ctggttccac tggagacagt gttggacatg ctccgggatg ccatggtggc caaagtcaat
      361 acttccaaag gcttcctgat tgatggctac ccgcgggagg tgcagcaagg agaagagttt
      421 gagcgacgga ttggacagcc cacactgctg ctgtatgtgg acgcaggccc tgagaccatg
      481 acccagcggc tcttgaaacg tggagagacc agcgggcgtg tggacgacaa tgaggagacc
      541 atcaaaaagc ggctggagac ctattacaag gccacagaac ccgtcatcgc cttctatgag
      601 aaacgtggca ttgtgcgcaa ggtcaacgct gagggctccg tggacagtgt cttctcccag
      661 gtctgcaccc acctggacgc cctaaagtac ccaactttct tgtaca
//
