LOCUS       sequence-29423-e         598 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-PNCK
FEATURES             Location/Qualifiers
     source          1..598
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    72..96
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(460..559)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(576..595)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(577..595)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat gagcaatgct
       61 tttttataat gccaactttg tacaaaaaag ttggcatgct gctgctgaag aaacacacgg
      121 aggacatcag cagcgtctac gagatccgcg agaggctcgg ctcggggccc tccccgctcc
      181 actctctctc cctcctgcct ctcctctctt cccacttcct tcccacctcc caccgccccg
      241 tctgcggcag gggtgccttc tccgaggtgg tgctggccca ggagcggggc tccgcacacc
      301 tcgtggccct caagtgcatc cccaagaagg ccctccgggg caaggaggcc ctggtggaga
      361 acgagatcgc agtgctccgt aggatcagtc accccaacat cgtcgctctg gaggatgtcc
      421 acgagagccc ttcccacctc tacctggcca tggaactgtg cccaactttc ttgtacaaag
      481 ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc
      541 aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg tattacat
//
