LOCUS       sequence-29451-e         763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-PAK1
FEATURES             Location/Qualifiers
     source          1..763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..110
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(645..662)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tagctcgggc ccaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat
       61 tgatgagcaa tgctttttta taatgccaac tttgtacaaa aaagttggca ccatgtcaaa
      121 taacggccta gacattcaag acaaaccccc agcccctccg atgagaaata ccagcactat
      181 gattggagcc ggcagcaaag atgctggaac cctaaaccat ggttctaaac ctctgcctcc
      241 aaacccagag gagaagaaaa agaaggaccg attttaccga tccattttac ctggagataa
      301 aacaaataaa aagaaagaga aagagcggcc agagatttct ctcccttcag attttgaaca
      361 cacaattcat gtcggttttg atgctgtcac aggggagttt acgggaatgc cagagcagtg
      421 ggcccgcttg cttcagacat caaatatcac taagtcggag cagaagaaaa acccgcaggc
      481 tgttctggat gtgttggagt tttacaactc gaagaagaca tccaacagcc agaaatacat
      541 gagctttaca gataagtcag ctgaggatta caattcttct aatgccttga atgtgaaggc
      601 tgtgtctgag actcctgcag tgccaccagt ttcagaagat gaggatgatg atgatgatga
      661 tgctacccca ccaccagtga ttgctccacg cccagagcac acaaaatctg tatacacacg
      721 gtctgtgatt gaaccacttc ctgtcactcc aactcgggac gtg
//
