LOCUS       sequence-29452-e         677 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-PIK3CB
FEATURES             Location/Qualifiers
     source          1..677
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    10..109
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             317..325
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 agctcgggcc caataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt
       61 gatgagcaat gcttttttat aatgccaact ttgtacaaaa aagttggcat gtgcttcagt
      121 ttcctaatgc ctcctgctat ggcagacatc cttgacatct gggcggtgga ttcacagata
      181 gcatctgatg gctccatacc tgtggatttc cttttgccca ctgggattta tatccagttg
      241 gaggtacctc gggaagctac catttcttat attaagcaga tgttatggaa gcaagttcac
      301 aattacccaa tgttcaacct ccttatggat attgactcct atatgtttgc atgtgtgaat
      361 cagactgctg tatatgagga gcttgaagat gaaacacgaa gactctgtga tgtcagacct
      421 tttcttccag ttctcaaatt agtgacaaga agttgtgacc caggggaaaa attagactca
      481 aaaattggag tccttatagg aaaaggtctg catgaatttg attccttgaa ggatcctgaa
      541 gtaaatgaat ttcgaagaaa aatgcgcaaa ttcagcgagg aaaaaatcct gtcacttgtg
      601 ggattgtctt ggatggactg gctaaaacaa acatatccac cagagcatga accatccatc
      661 cctgaaaact tagaaga
//
