LOCUS       sequence-29453-e         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-PIK4CA
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    72..96
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat gagcaatgct
       61 tttttataat gccaactttg tacaaaaaag ttggcatgcg ggagatggca ggggcctggc
      121 acatgacggt ggagcagaaa tttggcctgt tttctgctga gataaaggaa gcagaccccc
      181 tggctgcctc ggaagcaagt caacccaaac cctgtccccc cgaagtgacc ccccactaca
      241 tctggatcga cttcctggtg cagcggtttg agatcgccaa gtactgcagc tctgaccaag
      301 tggagatctt ctccagcctg ctgcagcgct ccatgtccct gaacatcggc ggggccaagg
      361 ggagcatgaa ccggcacgtg gcggccatcg ggccccgctt caagctgctg accctggggc
      421 tgtccctcct gcatgccgat gtggttccaa atgcaaccat ccgcaatgtg cttcgcgaga
      481 agatctactc cactgccttt gactacttca gctgtccccc aaagttccct actcaaggag
      541 agaagcggct gcgtgaagac ataagcatca tgattaaatt ttggaccgcc atgttctcag
      601 ataagaagta cctgaccgcc agccagcttg ttcccccaga taatcaggac acccggagca
      661 acctggacat aactgtcggc tctcggcaac aagccaccca aggctggatc aacacatacc
      721 ccctgtccag cggcatgtcc accatct
//
