LOCUS       sequence-29472-e         660 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-NLK
FEATURES             Location/Qualifiers
     source          1..660
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    8..107
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             149..172
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 ctcgggccca ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga
       61 tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa gttggcatgg cggcttacaa
      121 tggcggtaca tctgcagcag caacaggtca ccaccaccac catcaccacc accttccaca
      181 cctccctcct cctcacctgc accaccacca ccaccctcaa caccatcttc atccggggtc
      241 ggctgccgct gtacaccctg tacagcagca cacctcttcg gcagctgcgg cagccgcagc
      301 agcggctgca gctgcagcca tgttaaaccc tgggcaacaa cagccatatt tcccatcacc
      361 ggcaccgggg caggctcctg gaccagctgc agcagcccca gctcaggtac aggctgccgc
      421 agctgctaca gttaaggcgc accatcatca gcactcgcat catccacagc agcagctgga
      481 tattgagccg gatagaccta ttggatatgg agcctttggt gttgtctggt cagtaacaga
      541 tccnagagat ggaaagagag tagcgctcaa aaagatgccc aacgtcttcc agaatctggt
      601 ctcttgcaaa agggtcttcc gggaattgaa gatgttgtgt ttttttaagc atgataatgt
//
