LOCUS       sequence-29486-e         645 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-ADCK2
FEATURES             Location/Qualifiers
     source          1..645
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    72..96
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     97..114
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     98..114
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat gagcaatgct
       61 tttttataat gccaactttg tacaaaaaag caggcttgta aaacgacggc cagtaactat
      121 aacggtccta aggtagcgag gcctgggtgg cgaattcggc acgagggcgg gcctccggcc
      181 tgaggcccgg cgaggtgctg gagggagcgg ggcgcggatc cggcccagat gggcagctcc
      241 gtccgcgggg tcagggccgg gcttcggctt caccgcagcc tcgcctgagc gggcgcctct
      301 gaagtggagg gcgggccgcc tgggccgcgg gcctcgggag gatggtggcg ccctggcgcg
      361 tctccgtcag ggtttgcctg tcgcacctga ggtgcttcga gctcagacag ggactcagcc
      421 tcctgaggcc ctccgagtgc cctcgcgatg ccaggctctg ctggcttctg ctgggcactt
      481 tgcccaaggt cgtctccctg tgcggggacg tgggtgaggg ggcccctgac gttctgagtc
      541 ggcgaagggt ccgctgcagc ggggcggctg gcgcggggcc cgcggagagc ctcccccgag
      601 cgggacctct gggcggcgtc ttcctgcatc tccgcctctg gcttc
//
