LOCUS       sequence-29518-e         659 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-PLK2
FEATURES             Location/Qualifiers
     source          1..659
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    7..106
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             274..291
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tcgggcccaa taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat
       61 gagcaatgct tttttataat gccaactttg tacaaaaaag ttggcatgga gcttttgcgg
      121 actatcacct accagccagc cgccagcacc aaaatgtgcg agcaggcgct gggcaagggt
      181 tgcggagcag actcgaagaa gaagcggccg ccgcagcccc ccgaggaatc gcagccacct
      241 cagtcccagg cgcaagtgcc cccggcggcc cctcaccacc atcaccacca ttcgcactcg
      301 gggccggaga tctcgcggat tatcgtcgac cccacgactg ggaagcgcta ctgccggggc
      361 aaagtgctgg gaaagggtgg ctttgcaaaa tgttacgaga tgacagattt gacaaataac
      421 aaagtctacg ccgcaaaaat tattcctcac agcagagtag ctaaacctca tcaaagggaa
      481 aagattgaca aagaaataga gcttcacaga attcttcatc ataagcatgt agtgcagttt
      541 taccactact tcgaggacaa agaaaacatt tacattctct tggaatactg cagtagaagg
      601 tcaatggctc atattttgaa agcaagaaag gtgttgacag agccagaagt tcgatacta
//
