LOCUS       sequence-29538-e         660 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-PRKAA2
FEATURES             Location/Qualifiers
     source          1..660
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    72..96
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat gagcaatgct
       61 tttttataat gccaactttg tacaaaaaag ttggcatggc tgagaagcag aagcacgacg
      121 ggcgggtgaa gatcggacac tacgtgctgg gcgacacgct gggcgtcggc accttcggca
      181 aagtgaagat tggagaacat caattaacag gccataaagt ggcagttaaa atcttaaata
      241 gacagaagat tcgcagttta gatgttgttg gaaaaataaa acgagaaatt caaaatctaa
      301 aactctttcg tcatcctcat attatcaaac tataccaggt gatcagcact ccaacagatt
      361 tttttatggt aatggaatat gtgtctggag gtgaattatt tgactacatc tgtaagcatg
      421 gacgggttga agagatggaa gccaggcggc tctttcagca gattctgtct gctgtggatt
      481 actgtcatag gcatatggtt gttcatcgag acctgaaacc agagaatgtc ctgttggatg
      541 cacacatgaa tgccaagata gccgatttcg gattatctaa tatgatgtca gatggtgaat
      601 ttctgagaac tagttgcgga tctccaaatt atgcagcacc tgaagtcatc tcaggcagat
//
