LOCUS       sequence-295453-        5493 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 N (nucleocapsid).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5493)
  TITLE     SARS-CoV-2 protein Mammalian expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 5493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4134237
FEATURES             Location/Qualifiers
     source          1..5493
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1262..1282
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1283..1309
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    1362..1483
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1373..1392)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1427..1446
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      1598..1644
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      1674..1810
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    1855..1926
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     rep_origin      complement(2152..2740)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2232..2251)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(3486..3554,3555..4346)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3704..3723)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      4359..4495
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    4647..4718
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(4723..4769)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    4787..4835
                     /note="synthetic polyadenylation signal"
     enhancer        4916..5219
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5221..5423
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5374..5394
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 caccatgtct gataatggac cccaaaatca gcgaaatgca ccccgcatta cgtttggtgg
       61 accctcagat tcaactggca gtaaccagaa tggagaacgc agtggggcgc gatcaaaaca
      121 acgtcggccc caaggtttac ccaataatac tgcgtcttgg ttcaccgctc tcactcaaca
      181 tggcaaggaa gaccttaaat tccctcgagg acaaggcgtt ccaattaaca ccaatagcag
      241 tccagatgac caaattggct actaccgaag agctaccaga cgaattcgtg gtggtgacgg
      301 taaaatgaaa gatctcagtc caagatggta tttctactac ctaggaactg ggccagaagc
      361 tggacttccc tatggtgcta acaaagacgg catcatatgg gttgcaactg agggagcctt
      421 gaatacacca aaagatcaca ttggcacccg caatcctgct aacaatgctg caatcgtgct
      481 acaacttcct caaggaacaa cattgccaaa aggcttctac gcagaaggga gcagaggcgg
      541 cagtcaagcc tcttctcgtt cctcatcacg tagtcgcaac agttcaagaa attcaactcc
      601 aggcagcagt aggggaactt ctcctgctag aatggctggc aatggcggtg atgctgctct
      661 tgctttgctg ctgcttgaca gattgaacca gcttgagagc aaaatgtctg gtaaaggcca
      721 acaacaacaa ggccaaactg tcactaagaa atctgctgct gaggcttcta agaagcctcg
      781 gcaaaaacgt actgccacta aagcatacaa tgtaacacaa gctttcggca gacgtggtcc
      841 agaacaaacc caaggaaatt ttggggacca ggaactaatc agacaaggaa ctgattacaa
      901 acattggccg caaattgcac aatttgcccc cagcgcttca gcgttcttcg gaatgtcgcg
      961 cattggcatg gaagtcacac cttcgggaac gtggttgacc tacacaggtg ccatcaaatt
     1021 ggatgacaaa gatccaaatt tcaaagatca agtcattttg ctgaataagc atattgacgc
     1081 atacaaaaca ttcccaccaa cagagcctaa aaaggacaaa aagaagaagg ctgatgaaac
     1141 tcaagcctta ccgcagagac agaagaaaca gcaaactgtg actcttcttc ctgctgcaga
     1201 tttggatgat ttctccaaac aattgcaaca atccatgagc agtgctgact caactcaggc
     1261 cgaaaacctt tactttcagt cgtaccccta tgacgtacca gattatgcgt aagctagctt
     1321 gactgactga gatacagcgt accttcagct cacagacatg ataagataca ttgatgagtt
     1381 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     1441 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     1501 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
     1561 ctacaaatgt ggtattggcc catctctatc ggtatcgtag cataacccct tggggcctct
     1621 aaacgggtct tgaggggttt tttgtgcccc tcgggccgga ttgctatcta ccggcattgg
     1681 cgcagaaaaa aatgcctgat gcgacgctgc gcgtcttata ctcccacata tgccagattc
     1741 agcaacggat acggcttccc caacttgccc acttccatac gtgtcctcct taccagaaat
     1801 ttatccttaa ggtcgtcagc tatcctgcag gcgatctctc gatttcgatc aagacattcc
     1861 tttaatggtc ttttctggac accactaggg gtcagaagta gttcatcaaa ctttcttccc
     1921 tccctaatct cattggttac cttgggctat cgaaacttaa ttaaccagtc aagtcagcta
     1981 cttggcgaga tcgacttgtc tgggtttcga ctacgctcag aattgcgtca gtcaagttcg
     2041 atctggtcct tgctattgca cccgttctcc gattacgagt ttcatttaaa tcatgtgagc
     2101 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     2161 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     2221 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     2281 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     2341 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     2401 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     2461 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     2521 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     2581 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     2641 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     2701 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     2761 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     2821 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     2881 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     2941 ctcagcgatc tgtctatttc gttcatccat agttgcattt aaatttccga actctccaag
     3001 gccctcgtcg gaaaatcttc aaacctttcg tccgatccat cttgcaggct acctctcgaa
     3061 cgaactatcg caagtctctt ggccggcctt gcgccttggc tattgcttgg cagcgcctat
     3121 cgccaggtat tactccaatc ccgaatatcc gagatcggga tcacccgaga gaagttcaac
     3181 ctacatcctc aatcccgatc tatccgagat ccgaggaata tcgaaatcgg ggcgcgcctg
     3241 gtgtaccgag aacgatcctc tcagtgcgag tctcgacgat ccatatcgtt gcttggcagt
     3301 cagccagtcg gaatccagct tgggacccag gaagtccaat cgtcagatat tgtactcaag
     3361 cctggtcacg gcagcgtacc gatctgttta aacctagata ttgatagtct gatcggtcaa
     3421 cgtataatcg agtcctagct tttgcaaaca tctatcaaga gacaggatca gcaggaggct
     3481 ttcgcatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     3541 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     3601 gtgcgcgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     3661 gccccgaaga acgctttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     3721 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     3781 acttggttga gtattcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     3841 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     3901 cgattggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     3961 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     4021 cgatgcctgt agcaatggca acaaccttgc gtaaactatt aactggcgaa ctacttactc
     4081 tagcttcccg gcaacagttg atagactgga tggaggcgga taaagttgca ggaccacttc
     4141 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     4201 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     4261 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     4321 gtgcctcact gattaagcat tggtaaccga ttctaggtgc attggcgcag aaaaaaatgc
     4381 ctgatgcgac gctgcgcgtc ttatactccc acatatgcca gattcagcaa cggatacggc
     4441 ttccccaact tgcccacttc catacgtgtc ctccttacca gaaatttatc cttaagatcc
     4501 cgaatcgttt aaactcgact ctggctctat cgaatctccg tcgtttcgag cttacgcgaa
     4561 cagccgtggc gctcatttgc tcgtcgggca tcgaatctcg tcagctatcg tcagcttacc
     4621 tttttggcag cgatcgcggc tcccgacatc ttggaccatt agctccacag gtatcttctt
     4681 ccctctagtg gtcataacag cagcttcagc tacctctcaa ttcaaaaaac ccctcaagac
     4741 ccgtttagag gccccaaggg gttatgctat caatcgttgc gttacacaca caaaaaacca
     4801 acacacatcc atcttcgatg gatagcgatt ttattatcta actgctgatc gagtgtagcc
     4861 agatctagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     4921 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     4981 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     5041 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     5101 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     5161 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgc
     5221 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     5281 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     5341 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
     5401 gggaggtcta tataagcaga gctggtttag tgaaccgtca gatcagatct ttgtcgatcc
     5461 taccatccac tcgacacacc cgccagcggc cgc
//
