LOCUS       sequence-29562-e         734 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-IHPK2
FEATURES             Location/Qualifiers
     source          1..734
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             503..514
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgatg agcaatgctt
       61 ttttataatg ccaactttgt acaaaaaagt tagcatgagc ccagccttca gggccatgga
      121 tgtggagccc cgcgccaaag gcgtccttct ggagcccttt gtccaccagg tcggggggca
      181 ctcatgcgtg ctccgcttca atgagacaac cctgtgcaag cccctggtcc caagggaaca
      241 tcagttctac gagaccctcc ctgctgagat gcgcaaattc actccccagt acaaaggtgt
      301 ggtatctgtg cgctttgaag aagatgaaga caggaacttg tgtctaatag catatccatt
      361 gaaaggggac catggaattg tggacattgt agataattca gactgtgaac caaaaagtaa
      421 gctcctaagg tggacaacaa acaaaaaaca tcatgtctta gaaacagaaa agacccctaa
      481 ggactgggtg cgtcagcacc gtaaagagga gaaaatgaag agccataagt tagaagaaga
      541 atttgagtgg ctaaagaaat ctgaagtctt gtactacact gtagagaaga aggggaatat
      601 aagttcccag cttaaacact ataacccttg gagcatgaaa tgtcaccagc aacagttaca
      661 gagaatgaag gagaatgcaa agcatcggaa ccagtacaaa tttatcttac tggaaaacct
      721 gacttcccgc tatg
//
