LOCUS       sequence-29572-e         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-PRKAR2A
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    5..104
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gggcccaata atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga
       61 gcaatgcttt tttataatgc caactttgta caaaaaagtt ggcatgagcc acatccagat
      121 cccgccgggg ctcacggagc tgctgcaggg ctacacggtg gaggtgctgc gacagcagcc
      181 gcctgacctc gtcgaattcg cagtggagta cttcacccgc ctgcgcgagg cccgcgcccc
      241 agcctcagtc ctgcccgccg ccaccccacg ccagagcctg ggccaccccc cgccagaacc
      301 cggcccggac cgtgtcgccg acgccaaagg ggacagcgag tcggaggagg acgaggactt
      361 ggaagttcca gttcctagca gatttaatag acgagtatca gtctgtgctg agacctataa
      421 ccctgatgag gaagaggaag atacagatcc aagggtgatt catcctaaaa ctgatgaaca
      481 gagatgcaga cttcaggaag cttgcaaaga tattctcctt ttcaaaaatc ttgatcagga
      541 acagctttct caagttctcg atgccatgtt tgaaaggata gtcaaagctg atgagcatgt
      601 cattgaccaa ggagatgatg gagacaactt ttatgtcata gaacggggaa cttatgacat
      661 tttagtaaca aaagataatc aaacccgctc tgttggtcaa tatgacaacc gtggcagttt
      721 tggagaacta gctctgatgt acaacacccc gagagctgct accattgttg ctac
//
