LOCUS       sequence-29591-e         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-STK32A
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    5..104
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(603..702)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(719..738)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(720..738)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(743..759)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(743..759)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gggcccaata atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga
       61 gcaatgcttt tttataatgc caactttgta caaaaaagtt ggcatgggag ccaacacttc
      121 aagaaaacca ccagtgtttg atgaaaatga agatgtcaac tttgaccact ttgaaatttt
      181 gcgagccatt gggaaaggca gttttgggaa ggtctgcatt gtacagaaga atgataccaa
      241 gaagatgtac gcaatgaagt acatgaataa acaaaagtgc gtggagcgca atgaagtgag
      301 aaatgtcttc aaggaactcc agatcatgca gggtctggag caccctttcc tggttaattt
      361 gtggtattcc ttccaagatg aggaagacat gttcatggtg gtggacctcc tgctgggtgg
      421 agacctgcgt tatcacctgc aacagaacgt ccacttcaag gaagaaacag tgaagctctt
      481 catctgtgag ctggtcatgg ccctggacta cctgcagaac cagcgcatca ttcacaggga
      541 tatgaagcct gacaatattt tacttgacga acatgatacc tggctctcct acaagtccca
      601 ctgcccaact ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg
      661 caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc
      721 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca
      781 aatct
//
