LOCUS       sequence-29592-e         762 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-CALM3
FEATURES             Location/Qualifiers
     source          1..762
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    6..105
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             105..551
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPT
                     EAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAA
                     ELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     protein_bind    complement(553..652)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(669..688)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(670..688)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(693..709)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(693..709)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gggcccaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgatg
       61 agcaatgctt ttttataatg ccaactttgt acaaaaaagt tggcatggct gaccagctga
      121 ctgaggagca gattgcagag ttcaaggagg ccttctccct ctttgacaag gatggagatg
      181 gcactatcac caccaaggag ttggggacag tgatgagatc cctgggacag aaccccactg
      241 aagcagagct gcaggatatg atcaatgagg tggatgcaga tgggaacggg accattgact
      301 tcccggagtt cctgaccatg atggccagaa agatgaagga cacagacagt gaggaggaga
      361 tccgagaggc gttccgtgtc tttgacaagg atgggaatgg ctacatcagc gccgcagagc
      421 tgcgtcacgt aatgacgaac ctgggggaga agctgaccga tgaggaggtg gatgagatga
      481 tcagggaggc tgacatcgat ggagatggcc aggtcaatta tgaagagttt gtacagatga
      541 tgactgcaaa gtgcccaact ttcttgtaca aagttggcat tataagaaag cattgcttat
      601 caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag
      661 ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc
      721 ccgtgtctca aaatctctga tgttacattg cacaagataa aa
//
