LOCUS       sequence-29594-e         721 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-CKS1B
FEATURES             Location/Qualifiers
     source          1..721
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    71..95
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(333..432)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(449..468)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(450..468)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(473..489)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(473..489)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgatg agcaatgctt
       61 ttttataatg ccaactttgt acaaaaaagt tggcatgtcg cacaaacaaa tttactattc
      121 ggacaaatac gacgacgagg agtttgagta tcgacatgtc atgctgccca aggacatagc
      181 caagctggtc cctaaaaccc atctgatgtc tgaatctgaa tggaggaatc ttggcgttca
      241 gcagagtcag ggatgggtcc attatatgat ccatgaacca gaacctcaca tcttgctgtt
      301 ccggcgccca ctacccaaga aaccaaagaa atgcccaact ttcttgtaca aagttggcat
      361 tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa
      421 aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc
      481 tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa
      541 aaatatatca tcatgcctcc tctagaccag ccaggacaga aatgcctcga cttcgctgct
      601 acccaaggtt gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa cccagtggac
      661 ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc
      721 c
//
