LOCUS       sequence-295998-       10827 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Conditional expression of Synapsin-FLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10827)
  AUTHORS   Willems J, de Jong APH, Scheefhals N, Mertens E, Catsburg LAE, 
            Poorthuis RB, de Winter F, Verhaagen J, Meye FJ, MacGillavry HD
  TITLE     ORANGE: A CRISPR/Cas9-based genome editing toolbox for epitope 
            tagging of endogenous proteins in neurons.
  JOURNAL   PLoS Biol. 2020 Apr 10;18(4):e3000665. doi: 
            10.1371/journal.pbio.3000665.
  PUBMED    32275651
REFERENCE   2  (bases 1 to 10827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2020-04-10"; volume: "18(4):e3000665. doi"; pages: " 
            10.1371/journal.pbio.3000665"
COMMENT     Sequence Label: pFSW FLEx Synapsin-FLAG
FEATURES             Location/Qualifiers
     source          1..10827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..20)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        192..571
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        573..771
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     726..746
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             789..969
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1016..1141
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1634..1867
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2052..2096
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2394..2511
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2577..3024
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     protein_bind    complement(3031..3064)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(3115..3148)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(3163..3186)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    5320..5353
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    5404..5437
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5473..6061
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5526..5546)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5944..5955)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6064..6080)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6065..6081)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6586..6766
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6792..6809)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6798..7022
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7068..7496
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7155..7174)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7365..7386
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7505..7525)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7510..7839
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7690..7825
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7752..7771
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7887..7934
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7953..8327
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTNRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8457..8578
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8494..8513)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8548..8567
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8627..8643)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8627..8643)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8640..8662)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8651..8667
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8675..8681,8682..8699,8700..8705))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8720..8741
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8858..8875)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9029..9617)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9109..9128)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9788..10579,10580..10648))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10411..10430
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10649..10753)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
       61 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
      121 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
      181 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
      241 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      301 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      361 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      421 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      481 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      541 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
      601 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
      661 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
      721 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg
      781 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
      841 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
      901 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
      961 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     1021 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     1081 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     1141 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     1201 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     1261 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     1321 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     1381 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     1441 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     1501 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     1561 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     1621 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1681 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1741 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1801 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1861 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1921 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1981 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     2041 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     2101 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     2161 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     2221 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     2281 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     2341 gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa
     2401 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     2461 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     2521 tacagggaca gcagagatcc agtttggtta attaactgca gagggccctg cgtatgagtg
     2581 caagtgggtt ttaggaccag gatgaggcgg ggtgggggtg cctacctgac gaccgacccc
     2641 gacccactgg acaagcaccc aacccccatt ccccaaattg cgcatcccct atcagagagg
     2701 gggaggggaa acaggatgcg gcgaggcgcg tgcgcactgc cagcttcagc accgcggaca
     2761 gtgccttcgc ccccgcctgg cggcgcgcgc caccgccgcc tcagcactga aggcgcgctg
     2821 acgtcactcg ccggtccccc gcaaactccc cttcccggcc accttggtcg cgtccgcgcc
     2881 gccgccggcc cagccggacc gcaccacgcg aggcgcgaga taggggggca cgggcgcgac
     2941 catctgcgct gcggcgccgg cgactcagcg ctgcctcagt ctgcggtggg cagcggagga
     3001 gtcgtgtcgt gcctgagagc gcagtctaga ataacttcgt ataggatact ttatacgaag
     3061 ttatgcagaa tggtagctgg attgtagctg ctattagcaa tatgaaacct cttaataact
     3121 tcgtatagca tacattatac gaagttatac tagaccggtc tacttgtcat cgtcgtcttt
     3181 atagtcacca gatccgtcgg agaagaggct ggcgaaagac ttcctcaggc tgcggatggt
     3241 ctcagctttc acctcgtcct ggctaaggct gggcctggga ggggctggct ctggaaggtt
     3301 gaaggcattg gtcagagact gggatttgtt gagctggggg tgcgggggtc ccccggcagc
     3361 ggcggtgatg ggtggtggca catcctggct gggtttctgg gccagctgtg gtttggcggg
     3421 acgtccagca ggacctgggc cgctgggccg gggctgctgt gtggtgggag gcccagtgcg
     3481 aggtccggga cctgcctgac tggcctgacg agtgggacca gcagggcctg ggggtttttg
     3541 gggagggccc tggcgctgct gccctccggg tggggctcct gaggccttcg ttggagctgg
     3601 accagagata gatgcctgac gggtagcctg cggggccccc gcctgacgct gtggagatgg
     3661 ggaggctggt gggcgcgctg ctggaggtgc tccagggcct cctgccactg gccgcgactg
     3721 gcggccttga ccctgggtca ctggtgtggc ctgagaggca gactgctgag gtgctgcggt
     3781 gggacttggt aggcgctgag gcagagggct gccagctggc ggtccaaggc cagaaagatg
     3841 ttgctggcct tgtgggggtg ggcgctgctg cagcgggggt ccctggcgct gaggtcctgg
     3901 gcctggctgt ggagggcctc cctggggtgg gggtcgttgt tgagcaggag gacctgcagg
     3961 ctgctgggag gtctggcggc ccaaggtcag ggctcctggg gatgaagact ggctgtggga
     4021 gcccctgcca ggggaagcat cccgctgcgg ctgccgaggc agagcctgag tcatcttgtt
     4081 gaccacaagt tccacgatga gctgcttgtc ttcatcctgg tgatcaccaa tgagtggcat
     4141 ggaggagccc accacctcaa taatgtgatc ccttccgtcc ttgccatgca gcgcttccac
     4201 tgcgcagatg tcaagtcccc caaaaatctc tgagcacgtg tctacccaca acttgtacct
     4261 gtcagacatg gcaatctgct caagcatagc agaacctgta ttggtcttcc agttacccga
     4321 cactgatgtc ctcatgtagg ccttgtagtt ctgcccaatc ttctggacac gcacatcata
     4381 tttagcatca atgaagggct cagcagtggc atatgtctta gtcagtgcca caacacttgc
     4441 aatatcctgg aagtcatgtt ggttgtctac cttgaccttg cccatcccag aatgtgcgtg
     4501 gcccatcttc acaaccacag ggtatgttgt gctgctgagc atctctttgt gattaggata
     4561 gaaagtctga tcaatcagag ggaattcctc tgttccaagc ttcttgtgta gtcgaaccat
     4621 ctgggcaaac acccagggtt tgtcacagaa gttgtagaca gaatgcaaag agtttacact
     4681 ggggattcca gcatactgca gcccaatgac caaacttcgg tagtctccat tacgtgccat
     4741 gctgaaggca tgctggcgga tcagcacaaa gtctggcttc agagacctca caactttgac
     4801 tccattccga agaacttcca tgtccacaga gaatccacca ttggcatgag ccacaagatt
     4861 gagatcagag aattcagctt gctctacttt aatgtcaatt tctccatgga tcttcttccc
     4921 tttgaagtat tttgcccagt cggtgtgcgg ttcgtcgatg accagcagca ccctggcggc
     4981 ggcgcccccg cggcctgcgc ccccagagcc accgcccacc tgctcgctga aggtggcggc
     5041 tgcggctgct gtggtttgct tgaccgcgtt agacagcgac gagaagaagc cgccgccccc
     5101 cgaggaccca ggactagggg ctgctggaga agccactgga gcagctgtgg aggccctctc
     5161 ggcagaggct gtcgcggagc cgggcgtggc cccagggctg gcggccgagg gaggcggcgg
     5221 gggcggttgc gggcgctgca ggtctgtcat gtacccattc ggcagattgg ccatgaagtt
     5281 gctgtccgac aggcggcgcc gcaggtagtt catgctagca taacttcgta taaagtatcc
     5341 tatacgaagt tatttgcctt aacccagaaa ttatcactgt tattctttag aatggtgcaa
     5401 agaataactt cgtataatgt atgctatacg aagttatgga tccgttaaca attcgatatc
     5461 aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     5521 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     5581 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     5641 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     5701 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     5761 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     5821 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     5881 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     5941 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     6001 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     6061 catcgatacc gtcgacctcg agacctagaa aaacatggag caatcacaag tagcaataca
     6121 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
     6181 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     6241 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     6301 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca
     6361 ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     6421 gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg
     6481 agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc
     6541 ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta
     6601 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     6661 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     6721 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac
     6781 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     6841 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     6901 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     6961 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     7021 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     7081 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     7141 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     7201 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     7261 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     7321 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     7381 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     7441 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     7501 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     7561 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     7621 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     7681 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     7741 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     7801 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     7861 tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca
     7921 tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg
     7981 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccaaccgg ctcgggttct
     8041 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
     8101 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
     8161 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
     8221 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
     8281 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag
     8341 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     8401 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     8461 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     8521 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     8581 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     8641 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     8701 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     8761 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     8821 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     8881 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     8941 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     9001 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     9061 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     9121 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     9181 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     9241 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     9301 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     9361 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     9421 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     9481 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     9541 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     9601 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     9661 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     9721 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     9781 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     9841 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     9901 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     9961 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
    10021 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
    10081 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
    10141 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
    10201 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
    10261 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    10321 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    10381 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    10441 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    10501 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    10561 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    10621 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    10681 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    10741 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
    10801 tcccgatccc ctatggtgca ctctcag
//
