LOCUS       sequence-296000-        1257 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of N gene's CDS from 
            SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR223 SARS-CoV-2 N_nostop
FEATURES             Location/Qualifiers
     source          1..1257
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(190..201)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 atgtccgaca acggtcccca gaaccagcgt aacgctccaa ggatcacctt cggtggacct
       61 agcgactcca ccggcagcaa ccagaacgga gaacgctccg gagccagaag caagcagcgt
      121 aggccacagg gcctgcctaa caacaccgcc agctggttca ccgctctgac ccagcacgga
      181 aaggaagacc tgaagttccc aaggggacag ggcgtgccaa tcaacaccaa cagctctcca
      241 gacgaccaga tcggatacta caggcgtgcc accaggcgca tcagaggagg cgacggaaag
      301 atgaaggacc tgtctcctag gtggtacttc tactacctgg gcaccggacc agaggctggt
      361 ctgccatacg gcgccaacaa ggacggcatc atctgggtgg ctaccgaggg agctctgaac
      421 accccaaagg accacatcgg aacccgtaac cctgccaaca acgctgccat cgtgctgcag
      481 ctgccacagg gaaccaccct gcccaaggga ttctacgctg agggctccag gggtggaagc
      541 caggctagct ctcgttcttc cagccgttct aggaacagct cccgcaactc taccccagga
      601 agctccagag gcaccagccc agctcgtatg gccggcaacg gaggtgacgc cgctctggct
      661 ctgctgctgc tggaccgtct gaaccagctg gaaagcaaga tgtccggaaa gggtcagcag
      721 cagcagggcc agaccgtgac caagaagtct gccgctgagg cctctaagaa gcccaggcag
      781 aagaggaccg ctaccaaggc ctacaacgtg acccaggcct tcggaagacg tggacctgaa
      841 cagacccagg gaaacttcgg cgaccaggag ctgatccgcc agggaaccga ctacaagcac
      901 tggccacaga tcgctcagtt cgccccttct gctagcgcct tcttcggaat gtccaggatc
      961 ggaatggagg tgaccccttc cggcacctgg ctgacctaca ccggagctat caagctggac
     1021 gacaaggacc ccaacttcaa ggaccaggtg atcctgctga acaagcacat cgacgcctac
     1081 aagaccttcc cacctaccga gccaaagaag gacaagaaga agaaggccga cgaaacccag
     1141 gccctgccac agaggcagaa gaagcagcag accgtgaccc tgctgccagc tgctgacctg
     1201 gacgacttct ccaagcagct gcagcagtct atgagctctg ctgactccac ccaggct
//
