LOCUS       sequence-296003-        3303 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Individual component of Caffeic Acid biosynthesis pathway, hpaC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Khakhar A, Starker CG, Chamness JC, Lee N, Stokke S, Wang C, Swanson
            R, Rizvi F, Imaizumi T, Voytas DF
  TITLE     Building customizable auto-luminescent luciferase-based reporters in
            plants.
  JOURNAL   Elife. 2020 Mar 25;9. pii: 52786. doi: 10.7554/eLife.52786.
  PUBMED    32209230
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-03-25"; volume: "9. pii: 52786. doi"; pages: " 
            10.7554/eLife.52786"
COMMENT     Sequence Label: P526
FEATURES             Location/Qualifiers
     source          1..3303
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      80..668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     569..588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     822..839
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        964..1309
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1252..1272
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     terminator      1938..2138
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     promoter        2211..2315
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccttgacgg caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
       61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ctaactcttt
      181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgctcacagc
      421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
      781 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
      841 aagcgggaga gcgcccacct gccaggggac ggcgcgccct atggaggtca acatggtgga
      901 gcacgacact ctggtctact ccaaaaatgt caaagataca gtctcagaag atcaaagggc
      961 tattgagact tttcaacaaa ggataatttc gggaaacctc ctcggattcc attgcccagc
     1021 tatctgtcac ttcatcgaaa ggacagtaga aaaggaaggt ggctcctaca aatgccatca
     1081 ttgcgataaa ggaaaggcta tcattcaaga tctctctgcc gacagtggtc ccaaagatgg
     1141 acccccaccc acgaggagca tcgtggaaaa agaagaggtt ccaaccacgt ctacaaagca
     1201 agtggattga tgtgacatct ccactgacgt aagggatgac gcacaatccc actatccttc
     1261 gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggacac gctcgagtat
     1321 aagagctcat ttttacaaca attaccaaca acaacaaaca acaaacaaca ttacaattac
     1381 atttacaatt atcgatacaa tgcaattaga cgagcagcgt cttagatttc gtgatgctat
     1441 ggcttctctt tctgccgctg ttaatatcat tacaacggaa ggcgacgcag gtcaatgcgg
     1501 catcactgcc actgctgtct gcagcgtcac ggacacgcca ccgtctttga tggtttgcat
     1561 aaatgccaat tcagcaatga accctgtgtt ccaaggtaac ggcaagctct gtgtaaatgt
     1621 tctcaatcac gagcaagagt taatggcacg tcactttgca gggatgaccg ggatggcaat
     1681 ggaggaacgt ttttccctct cttgctggca aaaaggcccg ctggctcaac ctgtactgaa
     1741 aggatcactc gcctcccttg agggggaaat cagagatgtg caggctattg gcacacacct
     1801 cgtgtacctc gttgagatta agaacataat tttaagcgca gagggccacg ggctgatcta
     1861 ctttaagagg cgatttcatc cagttatgct ggaaatggag gctgcaatca gtggctgagc
     1921 ttgatccccc gtcgacagct agctatatca tcaatttatg tattacacat aatatcgcac
     1981 tcagtctttc atctacggca atgtaccagc tgatataatc agttattgaa atatttctga
     2041 atttaaactt gcatcaataa atttatgttt ttgcttggac tataatacct gacttgttat
     2101 tttatcaata aatatttaaa ctatatttct ttcaagatcg ctgttagagc tcccggtgac
     2161 gcaggtgatg aaaagcctta gggtcaggtg gcacttttcg gggaaatgtg cgatgaaccc
     2221 ctatttgttt atttttctaa atacattcaa atatgtatcc gctaatgaga caataaccct
     2281 gataaatgct tcaattatat tgaaaaagga agagtatgag tatccaacat ttccgtgtcg
     2341 cccttattcc cttttttgcg gccttctgcc ttcctgtttt tgctcaccca gaaacgctgg
     2401 tgaaagtaaa agatgctgaa gatcagttgg gtgcccgagt gggttacatt gaactggatc
     2461 tcaacagcgg caaaatctta gagagttttc gccccgaaga acgcttccca atgatgagca
     2521 ctttcaaagt tctgctatgt ggcgcggtgt tatcccgtat tgatgctggg caagagcaac
     2581 tcggtcgccg catacactat tctcagaatg acttggttga gtattcacca gtcacagaaa
     2641 agcatcttac ggatggcatg acagtaagag aattatgtag tgctgccata accatgagtg
     2701 ataacactgc ggccaactta cttctgacaa cgattggagg accgaaggag ctaaccgctt
     2761 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     2821 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaactctcc
     2881 gcaaactatt aactggcgaa ctacttactc ttgcttcacg ccagcaactc attgactgga
     2941 tggaggcgga caaagttgca ggaccacttc tgcgctcggc acttccggct ggctggttta
     3001 ttgctgataa atctggagcc ggtgagcgtg gctctcgcgg tatcattgca gcactggggc
     3061 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     3121 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     3181 cagacgaagg gaaataaata gtagcccgcc tgatgtgcgg gctttttttt ggtggtggtt
     3241 accagtgtcg cttagctacc gtaggtcctc ttaggcggtc cgattagcga catagtcatc
     3301 aat
//
