LOCUS       sequence-296004-        4386 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Individual component of Caffeic Acid biosynthesis pathway, RcTAL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Khakhar A, Starker CG, Chamness JC, Lee N, Stokke S, Wang C, Swanson
            R, Rizvi F, Imaizumi T, Voytas DF
  TITLE     Building customizable auto-luminescent luciferase-based reporters in
            plants.
  JOURNAL   Elife. 2020 Mar 25;9. pii: 52786. doi: 10.7554/eLife.52786.
  PUBMED    32209230
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-03-25"; volume: "9. pii: 52786. doi"; pages: " 
            10.7554/eLife.52786"
COMMENT     Sequence Label: P527
FEATURES             Location/Qualifiers
     source          1..4386
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      80..668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     569..588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     822..839
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        964..1309
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1252..1272
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     terminator      3021..3221
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     promoter        3294..3398
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccttgacgg caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
       61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ctaactcttt
      181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgctcacagc
      421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
      781 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
      841 aagcggaaga gcgcccacct gcaacgccgg ggcgcgccct atggaggtca acatggtgga
      901 gcacgacact ctggtctact ccaaaaatgt caaagataca gtctcagaag atcaaagggc
      961 tattgagact tttcaacaaa ggataatttc gggaaacctc ctcggattcc attgcccagc
     1021 tatctgtcac ttcatcgaaa ggacagtaga aaaggaaggt ggctcctaca aatgccatca
     1081 ttgcgataaa ggaaaggcta tcattcaaga tctctctgcc gacagtggtc ccaaagatgg
     1141 acccccaccc acgaggagca tcgtggaaaa agaagaggtt ccaaccacgt ctacaaagca
     1201 agtggattga tgtgacatct ccactgacgt aagggatgac gcacaatccc actatccttc
     1261 gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggacac gctcgagtat
     1321 aagagctcat ttttacaaca attaccaaca acaacaaaca acaaacaaca ttacaattac
     1381 atttacaatt atcgatacaa tgacgctcca atcacagact gctaaagact gccttgcatt
     1441 agatggcgct ttaacgctgg tgcaatgcga ggccatcgct acacataggt cccgaataag
     1501 cgtcactcct gccctcagag agagatgtgc aagggctcac gcccgtctgg aacacgccat
     1561 cgccgagcaa agacatatat acggcataac cactgggttt ggcccgcttg caaatcgact
     1621 gattggagca gatcaaggcg cagaactcca acaaaacctt atttaccacc ttgccacggg
     1681 ggttgggcca aaattgagtt gggctgaggc aagggccctg atgttggcca ggctgaactc
     1741 aattctccag ggagcttcag gggcttcccc agagacaatc gatcgtatcg tagcagtact
     1801 caacgctgga tttgcccccg aggttccggc tcaaggaact gtcggcgcaa gcggtgacct
     1861 gaccccgctc gcacacatgg tgctcgctct gcagggccga ggtcgtatga ttgacccaag
     1921 tggccgtgtt caagaggccg gagccgtcat ggatcgtctg tgcggtgggc ccctgaccct
     1981 ggctgccagg gacggactgg cactggtaaa cggaacttcc gctatgacgg ccattgccgc
     2041 cctcaccggt gtagaagctg ccagagccat agatgctgct ttaaggcact ccgctgtact
     2101 tatggaagta cttagcggac acgcagaggc atggcatcct gcctttgcag agcttcgtcc
     2161 tcatccaggt caattgagag ccacagagcg tcttgcacaa gccttagatg gcgccggtag
     2221 agtgtgtagg accttaactg ctgcaagacg tcttaccgcc gccgacctga ggcccgagga
     2281 ccatcccgca caggatgcct actcattgag agtagttccg caattagtag gggcagtctg
     2341 ggacacactt gattggcatg acagggtagt cacgtgcgag ttaaacagcg tcacagataa
     2401 ccctatattt cctgagggct gcgctgtgcc tgctctccat ggcgggaact ttatgggtgt
     2461 acatgtcgca ctcgcttctg atgcactgaa cgctgcattg gtaactctgg ctggtttagt
     2521 tgagaggcag atcgcaagac tgacagacga gaagttgaat aagggcttac cagcattcct
     2581 ccacggagga caggctggat tacaaagtgg gttcatgggg gcccaagtca ccgctactgc
     2641 tttgctcgcc gaaatgcgag ctaacgctac ccctgtctca gtacagtctc tttcaacaaa
     2701 tggcgcaaac caggacgttg tgtccatggg tacgattgca gccaggcgag cacgagcaca
     2761 attgttaccg ctgtcacaga ttcaggctat tttggcatta gctttggcac aagctatgga
     2821 tttgttagac gatccggaag gacaagcagg ctggtcactc actgccaggg acctcaggga
     2881 ccgaatcagg gctgtaagtc ccggattacg tgccgatagg ccacttgccg ggcacattga
     2941 ggcagtcgca caaggacttc gtcatccatc tgccgctgcc gaccctccgg ctagtggttg
     3001 agcttgatcc cccgtcgaca gctagctata tcatcaattt atgtattaca cataatatcg
     3061 cactcagtct ttcatctacg gcaatgtacc agctgatata atcagttatt gaaatatttc
     3121 tgaatttaaa cttgcatcaa taaatttatg tttttgcttg gactataata cctgacttgt
     3181 tattttatca ataaatattt aaactatatt tctttcaaga tcgctgttag agctcagtga
     3241 ctagcaggtg atgaaaagcc ttagggtcag gtggcacttt tcggggaaat gtgcgatgaa
     3301 cccctatttg tttatttttc taaatacatt caaatatgta tccgctaatg agacaataac
     3361 cctgataaat gcttcaatta tattgaaaaa ggaagagtat gagtatccaa catttccgtg
     3421 tcgcccttat tccctttttt gcggccttct gccttcctgt ttttgctcac ccagaaacgc
     3481 tggtgaaagt aaaagatgct gaagatcagt tgggtgcccg agtgggttac attgaactgg
     3541 atctcaacag cggcaaaatc ttagagagtt ttcgccccga agaacgcttc ccaatgatga
     3601 gcactttcaa agttctgcta tgtggcgcgg tgttatcccg tattgatgct gggcaagagc
     3661 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtattca ccagtcacag
     3721 aaaagcatct tacggatggc atgacagtaa gagaattatg tagtgctgcc ataaccatga
     3781 gtgataacac tgcggccaac ttacttctga caacgattgg aggaccgaag gagctaaccg
     3841 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     3901 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaactc
     3961 tccgcaaact attaactggc gaactactta ctcttgcttc acgccagcaa ctcattgact
     4021 ggatggaggc ggacaaagtt gcaggaccac ttctgcgctc ggcacttccg gctggctggt
     4081 ttattgctga taaatctgga gccggtgagc gtggctctcg cggtatcatt gcagcactgg
     4141 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     4201 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     4261 tgtcagacga agggaaataa atagtagccc gcctgatgtg cgggcttttt tttggtggtg
     4321 gttaccagtg tcgcttagct accgtaggtc ctcttaggcg gtccgattag cgacatagtc
     4381 atcaat
//
