LOCUS       sequence-296008-        3445 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes constitutive yellow (sfYFP) fluorescence for identification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Alnahhas RN, Winkle JJ, Hirning AJ, Karamched B, Ott W, Josic K, 
            Bennett MR
  TITLE     Spatiotemporal Dynamics of Synthetic Microbial Consortia in 
            Microfluidic Devices.
  JOURNAL   ACS Synth Biol. 2019 Sep 20;8(9):2051-2058. doi: 
            10.1021/acssynbio.9b00146. Epub 2019 Aug 9.
  PUBMED    31361464
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00146"; journalName: "ACS Synth Biol"; date: 
            "2019-09-20- 20"; volume: "8"; issue: "9"; pages: "2051-2058"
COMMENT     Sequence Label: Sender_plasmid1
FEATURES             Location/Qualifiers
     source          1..3445
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    20..36
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     25..47
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             238..267
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             520..1233
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      1419..1446
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(1542..1559)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1676..2221)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(1756..1775)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2335..2429)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2459..3250,3251..3319))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3082..3101
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3320..3424)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acctgacgtc tgtgtggaat tgtgagcgga taacaatttc acacagggcc ctcggacacc
       61 gaggagaatg tcaagaggcg aacacacaac gtcttggagc gccagaggag gaacgagcta
      121 aaacggagct tttttgccct gcgtgaccag atcccggagt tggaaaacaa tgaaaaggcc
      181 cccaaggtag ttatccttaa aaaagccaca gcatacatcc tgtccgtcca agcagaggag
      241 caaaagctca tttctgaaga ggacttgttg cggaaacgac gagaacagtt gaaacacaaa
      301 cttgaacagc tacggaactc ttgtgcgtaa ggaaaagtaa ggaaaacgat tccttctaac
      361 agaaatgtcc tgagcaatca cctatgaact gtcgatggtg caaaaccttt cgcggtatgg
      421 catgatagcg cccggaagca tgccacttaa aaaggagatc aacaatgaaa gcaattttcg
      481 tactgaaaca tcttaatcat gctaaggaga aatactagta tgcgtaaagg cgaagagctg
      541 ttcactggtg tcgtccctat tctggtggaa ctggatggtg atgtcaacgg tcataagttt
      601 tccgtgcgtg gcgagggtga aggtgacgca actaatggta aactgacgct gaagttcatc
      661 tgtactactg gtaaactgcc ggtaccttgg ccgactctgg taacgacgct gacttatggt
      721 gttcagtgct ttgctcgtta tccggaccat atgaagcagc atgacttctt caagtccgcc
      781 atgccggaag gctatgtgca ggaacgcacg atttccttta aggatgacgg cacgtacaaa
      841 acgcgtgcgg aagtgaaatt tgaaggcgat accctggtaa accgcattga gctgaaaggc
      901 attgacttta aagaagacgg caatatcctg ggccataagc tggaatacaa ttttaacagc
      961 cacaatgttt acatcaccgc cgataaacaa aaaaatggca ttaaagcgaa ttttaaaatt
     1021 cgccacaacg tggaggatgg cagcgtgcag ctggctgatc actaccagca aaacactcca
     1081 atcggtgatg gtcctgttct gctgccagac aatcactatc tgagctacca aagcgttctg
     1141 tctaaagatc cgaacgagaa acgcgatcat atggttctgc tggagttcgt aaccgcagcg
     1201 ggcatcacgc atggtatgga tgaactgtac aaagcagcga acgacgaaaa ttacgccgcg
     1261 tctgtttaat aaaagcttcc cctacgtgct gctgaagttg cccgcaacag agagtggaac
     1321 caaccggtga taccacgata ctatgactga gagtcaacgc catgagcggc ctcatttctt
     1381 attctgagtt acaacagtcc gcaccgctgt ctcacactgg ctcaccttcg ggtgggcctt
     1441 tctgcgttta tatactagag agagaatata aaaagccaga ttattaatcc ggctttttta
     1501 ttatttccta gctagctaga cctagggata tattccgctt cctcgctcac tgactcgcta
     1561 cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg aacggggcgg agatttcctg
     1621 gaagatgcca ggaagatact taacagggaa gtgagagggc cgcggcaaag ccgtttttcc
     1681 ataggctccg cccccctgac aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa
     1741 acccgacagg actataaaga taccaggcgt ttccccctgg cggctccctc gtgcgctctc
     1801 ctgttcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgt ttgtctcatt
     1861 ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg tatgcacgaa
     1921 ccccccgttc agtccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     1981 gaaagacatg caaaagcacc actggcagca gccactggta attgatttag aggagttagt
     2041 cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca agttttggtg actgcgctcc
     2101 tccaagccag ttacctcggt tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc
     2161 ctgcaaggcg gttttttcgt tttcagagca agagattacg cgcagaccaa aacgatctca
     2221 agaagatcat cttattaatc agataaaata tttctagatt tcagtgcaat ttatctcttc
     2281 aaatgtagca cctgaagtca gccccatacg atataagttg ttactagtgc ttggattctc
     2341 accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga
     2401 ggtcattact ggatctatca acaggagtcc aagcgagctc gtaaacttgg tctgacagtt
     2461 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     2521 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     2581 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     2641 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     2701 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     2761 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     2821 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     2881 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     2941 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     3001 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     3061 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     3121 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     3181 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     3241 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     3301 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     3361 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     3421 cgcgcacatt tccccgaaaa gtgcc
//
