LOCUS       sequence-296010-        3857 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes inducible expression of RhlI (C4-HSL quorum sensing 
            synthase).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Alnahhas RN, Winkle JJ, Hirning AJ, Karamched B, Ott W, Josic K, 
            Bennett MR
  TITLE     Spatiotemporal Dynamics of Synthetic Microbial Consortia in 
            Microfluidic Devices.
  JOURNAL   ACS Synth Biol. 2019 Sep 20;8(9):2051-2058. doi: 
            10.1021/acssynbio.9b00146. Epub 2019 Aug 9.
  PUBMED    31361464
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00146"; journalName: "ACS Synth Biol"; date: 
            "2019-09-20- 20"; volume: "8"; issue: "9"; pages: "2051-2058"
COMMENT     Sequence Label: Sender_plasmid2
FEATURES             Location/Qualifiers
     source          1..3857
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    65..81
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     70..92
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             283..312
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    447..463
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    478..494
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      1373..1400
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(1549..2364)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSSMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2272..2291
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2356..2375
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      2486..3074
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2975..2994
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3228..3245
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3260..3399
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3325..3344)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3485..3507
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3501..3692)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 gcgctcgagc ttcccaacct taccagaggg cgccccagct ggcaattccg acgtctgtgt
       61 ggaattgtga gcggataaca atttcacaca gggccctcgg acaccgagga gaatgtcaag
      121 aggcgaacac acaacgtctt ggagcgccag aggaggaacg agctaaaacg gagctttttt
      181 gccctgcgtg accagatccc ggagttggaa aacaatgaaa aggcccccaa ggtagttatc
      241 cttaaaaaag ccacagcata catcctgtcc gtccaagcag aggagcaaaa gctcatttct
      301 gaagaggact tgttgcggaa acgacgagaa cagttgaaac acaaacttga acagctacgg
      361 aactcttgtg cgtaaggaaa agtaaggaaa acgattcctt ctaacagaaa tgtcctgagc
      421 aatcacctat gaactgtcga ttgacattgt gagcggataa caagatactg agcacaattg
      481 tgagcggata acaattgcat gccacttaaa aaggagatca acaatgaaag caattttcgt
      541 actgaaacat cttaatcatg ctaaggagaa atactagtat gatcgaattg ctctctgaat
      601 cgctggaagg gctttccgcc gccatgatcg ccgagctggg acgctaccgg catcaggtct
      661 tcatcgagaa gctgggctgg gacgtggtct ccacctccag ggtccgcgac caggaattcg
      721 accagttcga ccatccgcaa acccgctaca tcgtcgccat gagccgccag ggcatctgcg
      781 gttgcgcccg cctgctgccg acgaccgacg cctacctgct caaggacgtc ttcgcctacc
      841 tgtgcagcga aaccccgccg agcgatccgt cggtctggga gctttcgcgc tacgccgcca
      901 gcgcggcgga cgatccgcag ctggcgatga agatattctg gtccagcctg caatgcgcct
      961 ggtacctggg cgccagttcg gtggtggcgg tgaccaccac ggccatggag cgctatttcg
     1021 ttcgcaacgg cgtgatcctc cagcgcctcg gcccgccgca gaaggtcaag ggcgagacgc
     1081 tggtcgcgat cagcttcccg gcctaccagg agcgcggcct ggagatgctg ctgcgctacc
     1141 acccggaatg gctgcagggc gtaccgctgt cgatggcggt gacctccgca gcgaacgacg
     1201 aaaattacgc ccttgcagcg taataaaagc ttcccctacg tgctgctgaa gttgcccgca
     1261 acagagagtg gaaccaaccg gtgataccac gatactatga ctgagagtca acgccatgag
     1321 cggcctcatt tcttattctg agttacaaca gtccgcaccg ctgtctcaca ctggctcacc
     1381 ttcgggtggg cctttctgcg tttatatact agagagagaa tataaaaagc cagattatta
     1441 atccggcttt tttattattt cctagctagc cggggaaatg tgcgcggaac ccctatttgt
     1501 ttatttttct aaatacattc aaatatgtat ccgctcatga attaattctt agaaaaactc
     1561 atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg
     1621 aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag
     1681 atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc
     1741 ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga
     1801 gaatggcaaa agtttatgca tttctttcca gacttgttca acaggccagc cattacgctc
     1861 gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag
     1921 acgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg
     1981 caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac
     2041 ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg
     2101 gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat
     2161 ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc
     2221 atcgggcttc ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc
     2281 ccatttatac ccatataaat cagcatccat gctggaattt aatcgcggcc tagagcaaga
     2341 cgtttcccgt tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag
     2401 ttttattgtt catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     2461 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     2521 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     2581 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     2641 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     2701 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     2761 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     2821 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     2881 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     2941 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     3001 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     3061 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     3121 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     3181 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     3241 gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     3301 cacaccgcaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     3361 tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc
     3421 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     3481 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag
     3541 ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc
     3601 gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc
     3661 atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg
     3721 ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat
     3781 gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg
     3841 cgggaccaga gctcagt
//
