LOCUS       sequence-296011-        3439 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes constitutive red (mCherry) fluorescence for identification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Alnahhas RN, Winkle JJ, Hirning AJ, Karamched B, Ott W, Josic K, 
            Bennett MR
  TITLE     Spatiotemporal Dynamics of Synthetic Microbial Consortia in 
            Microfluidic Devices.
  JOURNAL   ACS Synth Biol. 2019 Sep 20;8(9):2051-2058. doi: 
            10.1021/acssynbio.9b00146. Epub 2019 Aug 9.
  PUBMED    31361464
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00146"; journalName: "ACS Synth Biol"; date: 
            "2019-09-20- 20"; volume: "8"; issue: "9"; pages: "2051-2058"
COMMENT     Sequence Label: Receiver_plasmid1
FEATURES             Location/Qualifiers
     source          1..3439
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             102..131
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             384..1091
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(529..547)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     797..816
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      1277..1304
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(1400..1417)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1534..2079)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(1614..1633)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2193..2287)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2317..3108,3109..3177))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2940..2959
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3178..3282)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    3323..3339
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3328..3350
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 ccctgcgtga ccagatcccg gagttggaaa acaatgaaaa ggcccccaag gtagttatcc
       61 ttaaaaaagc cacagcatac atcctgtccg tccaagcaga ggagcaaaag ctcatttctg
      121 aagaggactt gttgcggaaa cgacgagaac agttgaaaca caaacttgaa cagctacgga
      181 actcttgtgc gtaaggaaaa gtaaggaaaa cgattccttc taacagaaat gtcctgagca
      241 atcacctatg aactgtcgat ggtgcaaaac ctttcgcggt atggcatgat agcgcccgga
      301 agcatgccac ttaaaaagga gatcaacaat gaaagcaatt ttcgtactga aacatcttaa
      361 tcatgctaag gagaaatact agtatggtga gcaagggcga ggaggataac atggccatca
      421 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg
      481 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
      541 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg
      601 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc
      661 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga
      721 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca
      781 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg
      841 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga
      901 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc
      961 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact
     1021 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg
     1081 agctgtacaa ggcagcgaac gacgaaaatt acgccgcgtc tgtttaataa aagcttcccc
     1141 tacgtgctgc tgaagttgcc cgcaacagag agtggaacca accggtgata ccacgatact
     1201 atgactgaga gtcaacgcca tgagcggcct catttcttat tctgagttac aacagtccgc
     1261 accgctgtct cacactggct caccttcggg tgggcctttc tgcgtttata tactagagag
     1321 agaatataaa aagccagatt attaatccgg cttttttatt atttcctagc tagctagacc
     1381 tagggatata ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt cgactgcggc
     1441 gagcggaaat ggcttacgaa cggggcggag atttcctgga agatgccagg aagatactta
     1501 acagggaagt gagagggccg cggcaaagcc gtttttccat aggctccgcc cccctgacaa
     1561 gcatcacgaa atctgacgct caaatcagtg gtggcgaaac ccgacaggac tataaagata
     1621 ccaggcgttt ccccctggcg gctccctcgt gcgctctcct gttcctgcct ttcggtttac
     1681 cggtgtcatt ccgctgttat ggccgcgttt gtctcattcc acgcctgaca ctcagttccg
     1741 ggtaggcagt tcgctccaag ctggactgta tgcacgaacc ccccgttcag tccgaccgct
     1801 gcgccttatc cggtaactat cgtcttgagt ccaacccgga aagacatgca aaagcaccac
     1861 tggcagcagc cactggtaat tgatttagag gagttagtct tgaagtcatg cgccggttaa
     1921 ggctaaactg aaaggacaag ttttggtgac tgcgctcctc caagccagtt acctcggttc
     1981 aaagagttgg tagctcagag aaccttcgaa aaaccgccct gcaaggcggt tttttcgttt
     2041 tcagagcaag agattacgcg cagaccaaaa cgatctcaag aagatcatct tattaatcag
     2101 ataaaatatt tctagatttc agtgcaattt atctcttcaa atgtagcacc tgaagtcagc
     2161 cccatacgat ataagttgtt actagtgctt ggattctcac caataaaaaa cgcccggcgg
     2221 caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac
     2281 aggagtccaa gcgagctcgt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     2341 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     2401 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     2461 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     2521 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     2581 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     2641 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     2701 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     2761 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     2821 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     2881 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     2941 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     3001 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     3061 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     3121 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     3181 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     3241 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     3301 gccacctgac gtctgtgtgg aattgtgagc ggataacaat ttcacacagg gccctcggac
     3361 accgaggaga atgtcaagag gcgaacacac aacgtcttgg agcgccagag gaggaacgag
     3421 ctaaaacgga gcttttttg
//
