LOCUS       sequence-296013-        3947 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes quorum sensing (C4-HSL) inducible cyan (sfCFP) expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Alnahhas RN, Winkle JJ, Hirning AJ, Karamched B, Ott W, Josic K, 
            Bennett MR
  TITLE     Spatiotemporal Dynamics of Synthetic Microbial Consortia in 
            Microfluidic Devices.
  JOURNAL   ACS Synth Biol. 2019 Sep 20;8(9):2051-2058. doi: 
            10.1021/acssynbio.9b00146. Epub 2019 Aug 9.
  PUBMED    31361464
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00146"; journalName: "ACS Synth Biol"; date: 
            "2019-09-20- 20"; volume: "8"; issue: "9"; pages: "2051-2058"
COMMENT     Sequence Label: Reciever_plasmid2
FEATURES             Location/Qualifiers
     source          1..3947
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    94..112
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             313..342
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    496..514
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    526..544
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             628..1341
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      1493..1520
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(1669..2484)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSSMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2392..2411
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2476..2495
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      2606..3194
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3095..3114
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3348..3365
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3380..3519
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3445..3464)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3605..3627
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3621..3812)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 ggtatggatg cggcgggacc agagctcagt gcgctcgagc ttcccaacct taccagaggg
       61 cgccccagct ggcaattccg acgtctgtgt ggatccctat cagtgataga gattcacaca
      121 gggccctcgg acaccgagga gaatgtcaag aggcgaacac acaacgtctt ggagcgccag
      181 aggaggaacg agctaaaacg gagctttttt gccctgcgtg accagatccc ggagttggaa
      241 aacaatgaaa aggcccccaa ggtagttatc cttaaaaaag ccacagcata catcctgtcc
      301 gtccaagcag aggagcaaaa gctcatttct gaagaggact tgttgcggaa acgacgagaa
      361 cagttgaaac acaaacttga acagctacgg aactcttgtg cgtaaggaaa agtaaggaaa
      421 acgattcctt ctaacagaaa tgtcctgagc aatcacctat gaactgtcga tcctgtgaaa
      481 tctggcagtt ttacttccct atcagtgata gagatactga gcacatccct atcagtgata
      541 gagatgcatg ccacttaaaa aggagatcaa caatgaaagc aattttcgta ctgaaacatc
      601 ttaatcatgc taaggagaaa tactagtatg cgtaaaggcg aagagctgtt cactggtgtc
      661 gtccctattc tggtggaact ggatggtgat gtcaacggtc ataagttttc cgtgcgtggc
      721 gagggtgaag gtgacgcaac taatggtaaa ctgacgctga agttcatctg tactactggt
      781 aaactgccgg taccttggcc gactctggta acgacgctga cttggggtgt tcagtgcttt
      841 gctcgttatc cggaccatat gaagcagcat gacttcttca agtccgccat gccggaaggc
      901 tatgtgcagg aacgcacgat ttcctttaag gatgacggca cgtacaaaac gcgtgcggaa
      961 gtgaaatttg aaggcgatac cctggtaaac cgcattgagc tgaaaggcat tgactttaaa
     1021 gaagacggca atatcctggg ccataagctg gaatacaatt acatcagcga caatgtttac
     1081 atcaccgccg ataaacaaaa aaatggcatt aaagcgaatt ttaaaattcg ccacaacgtg
     1141 gaggatggca gcgtgcagct ggctgatcac taccagcaaa acactccaat cggtgatggt
     1201 cctgttctgc tgccagacaa tcactatctg agcacgcaaa gcgttctgtc taaagatccg
     1261 aacgagaaac gcgatcatat ggttctgctg gagttcgtaa ccgcagcggg catcacgcat
     1321 ggtatggatg aactgtacaa atgatgaagc ttcccctacg tgctgctgaa gttgcccgca
     1381 acagagagtg gaaccaaccg gtgataccac gatactatga ctgagagtca acgccatgag
     1441 cggcctcatt tcttattctg agttacaaca gtccgcaccg ctgtctcaca ctggctcacc
     1501 ttcgggtggg cctttctgcg tttatatact agagagagaa tataaaaagc cagattatta
     1561 atccggcttt tttattattt cctagctagc cggggaaatg tgcgcggaac ccctatttgt
     1621 ttatttttct aaatacattc aaatatgtat ccgctcatga attaattctt agaaaaactc
     1681 atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg
     1741 aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag
     1801 atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc
     1861 ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga
     1921 gaatggcaaa agtttatgca tttctttcca gacttgttca acaggccagc cattacgctc
     1981 gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag
     2041 acgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg
     2101 caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac
     2161 ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg
     2221 gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat
     2281 ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc
     2341 atcgggcttc ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc
     2401 ccatttatac ccatataaat cagcatccat gctggaattt aatcgcggcc tagagcaaga
     2461 cgtttcccgt tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag
     2521 ttttattgtt catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     2581 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     2641 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     2701 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     2761 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     2821 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     2881 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     2941 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     3001 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     3061 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     3121 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     3181 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     3241 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     3301 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     3361 gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     3421 cacaccgcaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     3481 tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc
     3541 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     3601 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag
     3661 ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc
     3721 gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc
     3781 atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg
     3841 ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat
     3901 gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggc
//
