LOCUS       sequence-29603-e         766 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-RIOK1
FEATURES             Location/Qualifiers
     source          1..766
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    4..103
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(290..307)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ggcccaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgatgag
       61 caatgctttt ttataatgcc aactttgtac aaaaaagttg gcatggacta ccggcggctt
      121 ctcatgagcc gggtggtccc cgggcaattc gacgacgcgg actcctctga cagtgaaaac
      181 agagacttga agacagtcaa agagaaggat gacattctgt ttgaagacct tcaagacaat
      241 gtgaatgaga atggtgaagg tgaaatagaa gatgaggagg aggagggtta tgatgatgat
      301 gatgatgact gggactggga tgaaggagtt ggaaaactcg ccaagggtta tgtctggaat
      361 ggaggaagca acccacaggc aaatcgacag acctccgaca gcagttcagc caaaatgtct
      421 actccagcag acaaggtctt acggaaattt gagaataaaa ttaatttaga taagctaaat
      481 gttactgatt ccgtcataaa taaagtcacc gaaaagtcta gacaaaagga agcagatatg
      541 tatcgcatca aagataaggc agacagagca actgtagaac aggtgttgga tcccagaaca
      601 agaatgattt tattcaagat gttgactaga ggaatcataa cagagataaa tggctgcatt
      661 agcacaggaa aagaagctaa tgtataccat gctagcacag caaatggaga gagcagagca
      721 atcaaaattt ataaaacttc tattttggtg ttcaaagatc gggata
//
