LOCUS       sequence-296096-       11184 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector to overexpress the autism-associated PTEN mutation
            H93R as a GFP-PTEN fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11184)
  AUTHORS   Fricano-Kugler CJ, Getz SA, Williams MR, Zurawel AA, DeSpenza T Jr, 
            Frazel PW, Li M, O'Malley AJ, Moen EL, Luikart BW
  TITLE     Nuclear Excluded Autism-Associated Phosphatase and Tensin Homolog 
            Mutations Dysregulate Neuronal Growth.
  JOURNAL   Biol Psychiatry. 2018 Aug 15;84(4):265-277. doi: 
            10.1016/j.biopsych.2017.11.025. Epub 2017 Dec 2.
  PUBMED    29373119
REFERENCE   2  (bases 1 to 11184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.biopsych.2017.11.025"; journalName: "Biol Psychiatry"; 
            date: "2018-08-15- 15"; volume: "84"; issue: "4"; pages: "265-277"
COMMENT     Sequence Label: FUG-PtenH93R
FEATURES             Location/Qualifiers
     source          1..11184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2649..3860
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3697..3716
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      3908..3917
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3914..3916,3917..3919,3920..4630)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3914..3916,3917..3919,3920..4630)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3959..3980)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4220..4239)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4567..4588
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5876..6464
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5929..5949)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6347..6358)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6467..6483)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6468..6484)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6989..7169
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7195..7212)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7201..7425
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7471..7899
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7558..7577)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7768..7789
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7908..7928)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7913..8242
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8093..8228
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8155..8174
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8290..8337
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8356..8730
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8860..8981
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8897..8916)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8951..8970
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9030..9046)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9030..9046)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9043..9065)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9054..9070
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9078..9084,9085..9102,9103..9108))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9123..9144
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9261..9278)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9432..10020)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9512..9531)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10191..10982,10983..11051))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10814..10833
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11052..11156)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatcg catgctagct agttcgaacc ttaattaacc cgtgtcggct
     2641 ccagatctgg cctccgcgcc gggttttggc gcctcccgcg ggcgcccccc tcctcacggc
     2701 gagcgctgcc acgtcagacg aagggcgcag cgagcgtcct gatccttccg cccggacgct
     2761 caggacagcg gcccgctgct cataagactc ggccttagaa ccccagtatc agcagaagga
     2821 cattttagga cgggacttgg gtgactctag ggcactggtt ttctttccag agagcggaac
     2881 aggcgaggaa aagtagtccc ttctcggcga ttctgcggag ggatctccgt ggggcggtga
     2941 acgccgatga ttatataagg acgcgccggg tgtggcacag ctagttccgt cgcagccggg
     3001 atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga gtagcgggct
     3061 gctgggctgg ccggggcttt cgtggccgcc gggccgctcg gtgggacgga agcgtgtgga
     3121 gagaccgcca agggctgtag tctgggtccg cgagcaaggt tgccctgaac tgggggttgg
     3181 ggggagcgca gcaaaatggc ggctgttccc gagtcttgaa tggaagacgc ttgtgaggcg
     3241 ggctgtgagg tcgttgaaac aaggtggggg gcatggtggg cggcaagaac ccaaggtctt
     3301 gaggccttcg ctaatgcggg aaagctctta ttcgggtgag atgggctggg gcaccatctg
     3361 gggaccctga cgtgaagttt gtcactgact ggagaactcg gtttgtcgtc tgttgcgggg
     3421 gcggcagtta tggcggtgcc gttgggcagt gcacccgtac ctttgggagc gcgcgccctc
     3481 gtcgtgtcgt gacgtcaccc gttctgttgg cttataatgc agggtggggc cacctgccgg
     3541 taggtgtgcg gtaggctttt ctccgtcgca ggacgcaggg ttcgggccta gggtaggctc
     3601 tcctgaatcg acaggcgccg gacctctggt gaggggaggg ataagtgagg cgtcagtttc
     3661 tttggtcggt tttatgtacc tatcttctta agtagctgaa gctccggttt tgaactatgc
     3721 gctcggggtt ggcgagtgtg ttttgtgaag ttttttaggc accttttgaa atgtaatcat
     3781 ttgggtcaat atgtaatttt cagtgttaga ctagtaaatt gtccgctaaa ttctggccgt
     3841 ttttggcttt tttgttagac gaagcttggg ctgcaggtcg actctagagg atccccgggt
     3901 accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     3961 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     4021 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     4081 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     4141 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     4201 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     4261 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     4321 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     4381 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     4441 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     4501 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     4561 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     4621 gctgtacaag aaaggatcca tgacagcaat catcaaagag atcgttagca gaaacaaaag
     4681 gagatatcaa gaggatggat tcgacttaga cttgacctat atttatccaa acattattgc
     4741 tatgggattt cctgcagaaa gacttgaagg cgtatacagg aacaatattg atgatgtagt
     4801 aaggtttttg gattcaaagc ataaaaacca ttacaagata tacaatcttt gtgctgaaag
     4861 acattatgac accgccaaat ttaattgcag agttgcacaa tatccttttg aagaccgtaa
     4921 cccaccacag ctagaactta tcaaaccctt ttgtgaagat cttgaccaat ggctaagtga
     4981 agatgacaat catgttgcag caattcactg taaagctgga aagggacgaa ctggtgtaat
     5041 gatatgtgca tatttattac atcggggcaa atttttaaag gcacaagagg ccctagattt
     5101 ctatggggaa gtaaggacca gagacaaaaa gggagtaact attcccagtc agaggcgcta
     5161 tgtgtattat tatagctacc tgttaaagaa tcatctggat tatagaccag tggcactgtt
     5221 gtttcacaag atgatgtttg aaactattcc aatgttcagt ggcggaactt gcaatcctca
     5281 gtttgtggtc tgccagctaa aggtcaaaat ttactcgtcc aattcaggac ccacacgacg
     5341 ggaagacaag ttcatgtact ttgagttccc tcagccgtta cctgtgtgtg gtgatatcaa
     5401 agtagagttc ttccacaaac agaacaagat gctaaaaaag gacaaaatgt ttcacttttg
     5461 ggtaaataca ttcttcatac caggaccaga ggaaacctca gaaaaagtag aaaatggaag
     5521 tctatgtgat caagaaatcg atagcatttg cagtatagag cgtgcagata atgacaagga
     5581 atatctagta cttactttaa caaaaaatga tcttgacaaa gcaaataaag acaaagccaa
     5641 ccgatacttt tctccaaatt ttaaggtgaa gctgtacttc acaaaaacag tagaggagcc
     5701 gtcaaatcca gaggctagca gttcaacttc tgtaacacca gatgttagtg acaatgaacc
     5761 tgatcattat agatattctg acaccactga ctctgatcca gagaatgaac cttttgatga
     5821 agatcagcat acacaaatta caaaagtctg agaattcgat atcaagctta tcgataatca
     5881 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     5941 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     6001 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     6061 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     6121 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     6181 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     6241 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     6301 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     6361 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     6421 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc
     6481 tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga
     6541 ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt
     6601 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     6661 ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat
     6721 ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc cagggatcag
     6781 atatccactg acctttggat ggtgctacaa gctagtacca gttgagcaag agaaggtaga
     6841 agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc atgggatgga
     6901 tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat ttcatcacat
     6961 ggcccgagag ctgcatccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg
     7021 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     7081 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     7141 cttttagtca gtgtggaaaa tctctagcag ggcccgttta aacccgctga tcagcctcga
     7201 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     7261 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     7321 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     7381 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     7441 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     7501 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     7561 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     7621 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     7681 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     7741 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     7801 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     7861 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     7921 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     7981 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     8041 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     8101 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     8161 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     8221 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     8281 tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag
     8341 gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac
     8401 gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag
     8461 gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac
     8521 caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac
     8581 gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc
     8641 gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc
     8701 gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc
     8761 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     8821 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     8881 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     8941 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     9001 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     9061 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     9121 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     9181 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     9241 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     9301 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     9361 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     9421 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     9481 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     9541 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     9601 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     9661 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     9721 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     9781 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     9841 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     9901 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     9961 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
    10021 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
    10081 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
    10141 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
    10201 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
    10261 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
    10321 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
    10381 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
    10441 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
    10501 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
    10561 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
    10621 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    10681 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    10741 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    10801 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    10861 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    10921 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    10981 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    11041 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    11101 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    11161 tttccccgaa aagtgccacc tgac
//
