LOCUS       sequence-296118-       11246 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector to overexpress the autism-associated PTEN mutation
            F241S and GFP as two separate proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11246)
  AUTHORS   Fricano-Kugler CJ, Getz SA, Williams MR, Zurawel AA, DeSpenza T Jr, 
            Frazel PW, Li M, O'Malley AJ, Moen EL, Luikart BW
  TITLE     Nuclear Excluded Autism-Associated Phosphatase and Tensin Homolog 
            Mutations Dysregulate Neuronal Growth.
  JOURNAL   Biol Psychiatry. 2018 Aug 15;84(4):265-277. doi: 
            10.1016/j.biopsych.2017.11.025. Epub 2017 Dec 2.
  PUBMED    29373119
REFERENCE   2  (bases 1 to 11246)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.biopsych.2017.11.025"; journalName: "Biol Psychiatry"; 
            date: "2018-08-15- 15"; volume: "84"; issue: "4"; pages: "265-277"
COMMENT     Sequence Label: FUG-T2A-F241S
FEATURES             Location/Qualifiers
     source          1..11246
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             834..1014
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1061..1186
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1679..1912
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2097..2141
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2439..2556
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2648..3859
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3696..3715
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      3907..3916
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3913..3915,3916..3918,3919..4629)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3913..3915,3916..3918,3919..4629)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3958..3979)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4219..4238)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4566..4587
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4630..4683
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    5938..6526
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5991..6011)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6409..6420)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6529..6545)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6530..6546)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7051..7231
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7257..7274)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7263..7487
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7533..7961
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7620..7639)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7830..7851
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7970..7990)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7975..8304
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8155..8290
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8217..8236
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8352..8399
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8418..8792
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8922..9043
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8959..8978)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9013..9032
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9092..9108)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9092..9108)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9105..9127)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9116..9132
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9140..9146,9147..9164,9165..9170))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9185..9206
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9323..9340)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9494..10082)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9574..9593)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10253..11044,11045..11113))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10876..10895
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11114..11218)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gtttgcctgt actgggtctc
      841 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      901 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      961 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     1021 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     1081 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     1141 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     1201 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     1261 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     1321 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     1381 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     1441 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     1501 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     1561 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     1621 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     1681 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     1741 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     1801 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     1861 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     1921 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     1981 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     2041 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     2101 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     2161 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     2221 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     2281 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     2341 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg gcactgcgtg
     2401 cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
     2461 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     2521 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     2581 gatccagttt ggttaatcgc atgctagcta gttcgaacct taattaaccc gtgtcggctc
     2641 cagatctggc ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct cctcacggcg
     2701 agcgctgcca cgtcagacga agggcgcagc gagcgtcctg atccttccgc ccggacgctc
     2761 aggacagcgg cccgctgctc ataagactcg gccttagaac cccagtatca gcagaaggac
     2821 attttaggac gggacttggg tgactctagg gcactggttt tctttccaga gagcggaaca
     2881 ggcgaggaaa agtagtccct tctcggcgat tctgcggagg gatctccgtg gggcggtgaa
     2941 cgccgatgat tatataagga cgcgccgggt gtggcacagc tagttccgtc gcagccggga
     3001 tttgggtcgc ggttcttgtt tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg
     3061 ctgggctggc cggggctttc gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag
     3121 agaccgccaa gggctgtagt ctgggtccgc gagcaaggtt gccctgaact gggggttggg
     3181 gggagcgcag caaaatggcg gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg
     3241 gctgtgaggt cgttgaaaca aggtgggggg catggtgggc ggcaagaacc caaggtcttg
     3301 aggccttcgc taatgcggga aagctcttat tcgggtgaga tgggctgggg caccatctgg
     3361 ggaccctgac gtgaagtttg tcactgactg gagaactcgg tttgtcgtct gttgcggggg
     3421 cggcagttat ggcggtgccg ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg
     3481 tcgtgtcgtg acgtcacccg ttctgttggc ttataatgca gggtggggcc acctgccggt
     3541 aggtgtgcgg taggcttttc tccgtcgcag gacgcagggt tcgggcctag ggtaggctct
     3601 cctgaatcga caggcgccgg acctctggtg aggggaggga taagtgaggc gtcagtttct
     3661 ttggtcggtt ttatgtacct atcttcttaa gtagctgaag ctccggtttt gaactatgcg
     3721 ctcggggttg gcgagtgtgt tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt
     3781 tgggtcaata tgtaattttc agtgttagac tagtaaattg tccgctaaat tctggccgtt
     3841 tttggctttt ttgttagacg aagcttgggc tgcaggtcga ctctagagga tccccgggta
     3901 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     3961 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     4021 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     4081 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     4141 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     4201 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     4261 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     4321 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     4381 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     4441 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     4501 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     4561 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     4621 ctgtacaagg agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc
     4681 cctaagtaca agaaaggatc catgacagcc atcatcaaag agatcgttag cagaaacaaa
     4741 aggagatatc aagaggatgg attcgactta gacttgacct atatttatcc aaacattatt
     4801 gctatgggat ttcctgcaga aagacttgaa ggcgtataca ggaacaatat tgatgatgta
     4861 gtaaggtttt tggattcaaa gcataaaaac cattacaaga tatacaatct ttgtgctgaa
     4921 agacattatg acaccgccaa atttaattgc agagttgcac aatatccttt tgaagaccat
     4981 aacccaccac agctagaact tatcaaaccc ttttgtgaag atcttgacca atggctaagt
     5041 gaagatgaca atcatgttgc agcaattcac tgtaaagctg gaaagggacg aactggtgta
     5101 atgatatgtg catatttatt acatcggggc aaatttttaa aggcacaaga ggccctagat
     5161 ttctatgggg aagtaaggac cagagacaaa aagggagtaa ctattcccag tcagaggcgc
     5221 tatgtgtatt attatagcta cctgttaaag aatcatctgg attatagacc agtggcactg
     5281 ttgtttcaca agatgatgtt tgaaactatt ccaatgttca gtggcggaac ttgcaatcct
     5341 cagtttgtgg tctgccagct aaaggtcaaa atttactcgt ccaattcagg acccacacga
     5401 cgggaagaca agttcatgta ctctgagttc cctcagccgt tacctgtgtg tggtgatatc
     5461 aaagtagagt tcttccacaa acagaacaag atgctaaaaa aggacaaaat gtttcacttt
     5521 tgggtaaata cattcttcat accaggacca gaggaaacct cagaaaaagt agaaaatgga
     5581 agtctatgtg atcaagaaat cgatagcatt tgcagtatag agcgtgcaga taatgacaag
     5641 gaatatctag tacttacttt aacaaaaaat gatcttgaca aagcaaataa agacaaagcc
     5701 aaccgatact tttctccaaa ttttaaggtg aagctgtact tcacaaaaac agtagaggag
     5761 ccgtcaaatc cagaggctag cagttcaact tctgtaacac cagatgttag tgacaatgaa
     5821 cctgatcatt atagatattc tgacaccact gactctgatc cagagaatga accttttgat
     5881 gaagatcagc atacacaaat tacaaaagtc tgagaattcg atatcaagct tatcgataat
     5941 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     6001 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     6061 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     6121 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     6181 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     6241 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     6301 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     6361 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     6421 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     6481 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga
     6541 cctcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct
     6601 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag
     6661 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     6721 aaggggggac tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg
     6781 atctaccaca cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc
     6841 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta
     6901 gaagaagcca atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg
     6961 gatgacccgg agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac
     7021 atggcccgag agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc
     7081 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     7141 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     7201 cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct gatcagcctc
     7261 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     7321 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     7381 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     7441 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     7501 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     7561 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     7621 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     7681 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     7741 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     7801 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     7861 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     7921 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     7981 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     8041 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     8101 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     8161 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     8221 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     8281 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     8341 gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca
     8401 aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg
     8461 acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg
     8521 aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg
     8581 accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt
     8641 acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga
     8701 ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact
     8761 gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg
     8821 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     8881 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     8941 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     9001 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     9061 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     9121 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     9181 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     9241 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     9301 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     9361 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     9421 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     9481 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     9541 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     9601 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     9661 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     9721 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     9781 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     9841 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     9901 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     9961 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
    10021 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
    10081 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
    10141 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
    10201 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
    10261 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
    10321 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
    10381 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
    10441 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
    10501 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
    10561 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
    10621 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
    10681 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
    10741 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    10801 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    10861 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    10921 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    10981 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    11041 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    11101 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    11161 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    11221 catttccccg aaaagtgcca cctgac
//
