LOCUS       sequence-296132-        1494 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression construct for producing Avi tagged Alanyl-tRNA 
            synthetase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1494)
  TITLE     Graslund lab plasmids
REFERENCE   2  (bases 1 to 1494)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AARS_C1_pNIC-Bio3
FEATURES             Location/Qualifiers
     source          1..1494
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1450..1494
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgg actctactct aacagcaagt gaaatccggc agcgatttat agatttcttc
      121 aagaggaacg agcatacgta tgttcactcg tctgccacca tcccattgga tgaccccact
      181 ttgctctttg ccaatgcagg catgaaccag tttaaaccca ttttcctgaa cacaattgac
      241 ccatctcacc ccatggcaaa gctgagcaga gctgccaata cccagaagtg catccgggct
      301 gggggcaaac ataatgacct ggacgatgtg ggcaaggatg tctatcatca caccttcttc
      361 gagatgctgg gctcttggtc ttttggagat tactttaagg aattggcatg taagatggct
      421 ctggaactcc tcacccaaga gtttggcatt cccattgaaa gactttatgt tacttacttt
      481 ggcggggatg aagcagctgg cttagaagca gatctggaat gcaaacagat ctggcaaaat
      541 ttggggctgg atgacaccaa aatcctccca ggcaacatga aggataactt ctgggagatg
      601 ggtgacacgg gcccctgtgg tccttgcagt gagatccact acgaccggat tggtggtcgg
      661 gacgccgcac atcttgtcaa ccaggacgac cctaatgtgc tggagatctg gaaccttgtg
      721 ttcatccagt ataacaggga agctgatggc attctgaaac ctcttcccaa gaaaagcatt
      781 gacacaggga tgggcctgga acgactggta tctgtgctgc agaataagat gtccaactat
      841 gacactgacc tttttgtccc ttactttgaa gccattcaga agggcacagg tgcccgacca
      901 tacactggga aagttggtgc tgaggatgcc gatgggattg acatggccta ccgggtgctg
      961 gctgaccatg ctcggaccat cactgtggca ctggctgatg gtggccggcc tgacaacaca
     1021 gggcgtggat atgtgttgag acggattctc cgccgagctg tccgatacgc ccatgaaaag
     1081 ctcaatgcca gcaggggctt ctttgctacg ttagtggatg ttgtcgtcca gtccctggga
     1141 gatgcatttc ctgagctgaa gaaggaccca gacatggtga aggacatcat taatgaagaa
     1201 gaggtgcagt ttctcaagac tctcagcaga gggcgtcgca tcctggacag gaaaattcag
     1261 agcctgggag acagcaagac cattcccgga gacactgctt ggctcctcta tgacacctat
     1321 gggtttccag tggatctgac tggactgatt gctgaagaga agggcctggt ggtagacatg
     1381 gatggctttg aagaggagag gaaactggcc cagctgaaat cacagggcaa gagcagtaaa
     1441 ggtggatacg gcctgaatga tatctttgaa gcgcagaaga ttgaatggca tgaa
//
