LOCUS       sequence-296144-         888 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression construct for producing Avi tagged Leucyl-tRNA 
            synthetase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 888)
  TITLE     Graslund lab plasmids
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: LARS_pNIC-Bio3
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             844..888
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgg gacctcagga atatacttta ctcaaattga aggtgcttga gccataccca
      121 tctaaattaa gtggcctgaa aggtaaaaat attttcttgg tggctgctac tctcagacct
      181 gagaccatgt ttgggcagac aaattgttgg gttcgtcctg atatgaagta cattggattt
      241 gagacggtga atggtgatat attcatctgt acccaaaaag cagccaggaa tatgtcatac
      301 cagggcttta ccaaagacaa tggcgtggtg cctgttgtta aggaattaat gggggaggaa
      361 attcttggtg catcactttc tgcaccttta acatcataca aggtgatcta tgttctccca
      421 atgctaacta ttaaggagga taaaggcact ggtgtggtta caagtgttcc ttccgactcc
      481 cctgatgata ttgctgccct cagagacttg aagaaaaagc aagccttacg agcaaaatat
      541 ggaattagag atgacatggt cttgccattt gagccggtgc cagtcattga aatcccaggt
      601 tttggaaatc tttctgctgt aaccatttgt gatgagttga aaattcagag ccagaatgac
      661 cgggaaaaac ttgcagaagc aaaggagaag atatatctaa aaggatttta tgagggtatc
      721 atgttggtgg atggatttaa aggacagaag gttcaagatg taaagaagac tattcagaaa
      781 aagatgattg acgctggaga tgcacttatt tacatggaac cagagagcag taaaggtgga
      841 tacggcctga atgatatctt tgaagcgcag aagattgaat ggcatgaa
//
