LOCUS       sequence-296171-       11289 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For plant prime editing in wheat plants or monocotyledons 
            protoplasts.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11289)
  AUTHORS   Lin Q, Zong Y, Xue C, Wang S, Jin S, Zhu Z, Wang Y, Anzalone AV, 
            Raguram A, Doman JL, Liu DR, Gao C
  TITLE     Prime genome editing in rice and wheat.
  JOURNAL   Nat Biotechnol. 2020 May;38(5):582-585. doi: 
            10.1038/s41587-020-0455-x. Epub 2020 Mar 16.
  PUBMED    32393904
REFERENCE   2  (bases 1 to 11289)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0455-x"; journalName: "Nat Biotechnol"; date: 
            "2020-05"; volume: "38"; issue: "5"; pages: "582-585"
COMMENT     Sequence Label: nCas9-PPE
FEATURES             Location/Qualifiers
     source          1..11289
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1997..2017
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2018..4531,4532..4534,4535..6118)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             6031..6039
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             6125..6145
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8297..8317
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8377..8553
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(9107..9123)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9107..9123)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9120..9142)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9131..9147
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9155..9161,9162..9179,9180..9185))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9200..9221
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9338..9355)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9509..10097)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9589..9608)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10268..11059,11060..11128))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10891..10910
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11129..11233)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgacccggtc gtgcccctct ctagagataa tgagcattgc atgtctaagt tataaaaaat
       61 taccacatat tttttttgtc acacttgttt gaagtgcagt ttatctatct ttatacatat
      121 atttaaactt tactctacga ataatataat ctatagtact acaataatat cagtgtttta
      181 gagaatcata taaatgaaca gttagacatg gtctaaagga caattgagta ttttgacaac
      241 aggactctac agttttatct ttttagtgtg catgtgttct cctttttttt tgcaaatagc
      301 ttcacctata taatacttca tccattttat tagtacatcc atttagggtt tagggttaat
      361 ggtttttata gactaatttt tttagtacat ctattttatt ctattttagc ctctaaatta
      421 agaaaactaa aactctattt tagttttttt atttaataat ttagatataa aatagaataa
      481 aataaagtga ctaaaaatta aacaaatacc ctttaagaaa ttaaaaaaac taaggaaaca
      541 tttttcttgt ttcgagtaga taatgccagc ctgttaaacg ccgtcgacga gtctaacgga
      601 caccaaccag cgaaccagca gcgtcgcgtc gggccaagcg aagcagacgg cacggcatct
      661 ctgtcgctgc ctctggaccc ctctcgatcg agagttccgc tccaccgttg gacttgctcc
      721 gctgtcggca tccagaaatt gcgtggcgga gcggcagacg tgagccggca cggcaggcgg
      781 cctcctcctc ctctcacggc accggcagct acgggggatt cctttcccac cgctccttcg
      841 ctttcccttc ctcgcccgcc gtaataaata gacaccccct ccacaccctc tttccccaac
      901 ctcgtgttgt tcggagcgca cacacacaca accagatctc ccccaaatcc acccgtcggc
      961 acctccgctt caaggtacgc cgctcgtcct cccccccccc ccctctctac cttctctaga
     1021 tcggcgttcc ggtccatggt tagggcccgg tagttctact tctgttcatg tttgtgttag
     1081 atccgtgttt gtgttagatc cgtgctgcta gcgttcgtac acggatgcga cctgtacgtc
     1141 agacacgttc tgattgctaa cttgccagtg tttctctttg gggaatcctg ggatggctct
     1201 agccgttccg cagacgggat cgatttcatg attttttttg tttcgttgca tagggtttgg
     1261 tttgcccttt tcctttattt caatatatgc cgtgcacttg tttgtcgggt catcttttca
     1321 tgcttttttt tgtcttggtt gtgatgatgt ggtctggttg ggcggtcgtt ctagatcgga
     1381 gtagaattaa ttctgtttca aactacctgg tggatttatt aattttggat ctgtatgtgt
     1441 gtgccataca tattcatagt tacgaattga agatgatgga tggaaatatc gatctaggat
     1501 aggtatacat gttgatgcgg gttttactga tgcatataca gagatgcttt ttgttcgctt
     1561 ggttgtgatg atgtggtgtg gttgggcggt cgttcattcg ttctagatcg gagtagaata
     1621 ctgtttcaaa ctacctggtg tatttattaa ttttggaact gtatgtgtgt gtcatacatc
     1681 ttcatagtta cgagtttaag atggatggaa atatcgatct aggataggta tacatgttga
     1741 tgtgggtttt actgatgcat atacatgatg gcatatgcag catctattca tatgctctaa
     1801 ccttgagtac ctatctatta taataaacaa gtatgtttta taattatttt gatcttgata
     1861 tacttggatg atggcatatg cagcagctat atgtggattt ttttagccct gccttcatac
     1921 gctatttatt tgcttggtac tgtttctttt gtcgatgctc accctgttgt ttggtgttac
     1981 ttctgcaaag cttatgccta agaaaaagag aaaagtggac aagaagtact cgatcggcct
     2041 cgatattggg actaactctg ttggctgggc cgtgatcacc gacgagtaca aggtgccctc
     2101 aaagaagttc aaggtcctgg gcaacaccga tcggcattcc atcaagaaga atctcattgg
     2161 cgctctcctg ttcgacagcg gcgagacggc tgaggctacg cggctcaagc gcaccgcccg
     2221 caggcggtac acgcgcagga agaatcgcat ctgctacctg caggagattt tctccaacga
     2281 gatggcgaag gttgacgatt ctttcttcca caggctggag gagtcattcc tcgtggagga
     2341 ggataagaag cacgagcggc atccaatctt cggcaacatt gtcgacgagg ttgcctacca
     2401 cgagaagtac cctacgatct accatctgcg gaagaagctc gtggactcca cagataaggc
     2461 ggacctccgc ctgatctacc tcgctctggc ccacatgatt aagttcaggg gccatttcct
     2521 gatcgagggg gatctcaacc cggacaatag cgatgttgac aagctgttca tccagctcgt
     2581 gcagacgtac aaccagctct tcgaggagaa ccccattaat gcgtcaggcg tcgacgcgaa
     2641 ggctatcctg tccgctaggc tctcgaagtc tcggcgcctc gagaacctga tcgcccagct
     2701 gccgggcgag aagaagaacg gcctgttcgg gaatctcatt gcgctcagcc tggggctcac
     2761 gcccaacttc aagtcgaatt tcgatctcgc tgaggacgcc aagctgcagc tctccaagga
     2821 cacatacgac gatgacctgg ataacctcct ggcccagatc ggcgatcagt acgcggacct
     2881 gttcctcgct gccaagaatc tgtcggacgc catcctcctg tctgatattc tcagggtgaa
     2941 caccgagatt acgaaggctc cgctctcagc ctccatgatc aagcgctacg acgagcacca
     3001 tcaggatctg accctcctga aggcgctggt caggcagcag ctccccgaga agtacaagga
     3061 gatcttcttc gatcagtcga agaacggcta cgctgggtac attgacggcg gggcctctca
     3121 ggaggagttc tacaagttca tcaagccgat tctggagaag atggacggca cggaggagct
     3181 gctggtgaag ctcaatcgcg aggacctcct gaggaagcag cggacattcg ataacggcag
     3241 catcccacac cagattcatc tcggggagct gcacgctatc ctgaggaggc aggaggactt
     3301 ctaccctttc ctcaaggata accgcgagaa gatcgagaag attctgactt tcaggatccc
     3361 gtactacgtc ggcccactcg ctaggggcaa ctcccgcttc gcttggatga cccgcaagtc
     3421 agaggagacg atcacgccgt ggaacttcga ggaggtggtc gacaagggcg ctagcgctca
     3481 gtcgttcatc gagaggatga cgaatttcga caagaacctg ccaaatgaga aggtgctccc
     3541 taagcactcg ctcctgtacg agtacttcac agtctacaac gagctgacta aggtgaagta
     3601 tgtgaccgag ggcatgagga agccggcttt cctgtctggg gagcagaaga aggccatcgt
     3661 ggacctcctg ttcaagacca accggaaggt cacggttaag cagctcaagg aggactactt
     3721 caagaagatt gagtgcttcg attcggtcga gatctctggc gttgaggacc gcttcaacgc
     3781 ctccctgggg acctaccacg atctcctgaa gatcattaag gataaggact tcctggacaa
     3841 cgaggagaat gaggatatcc tcgaggacat tgtgctgaca ctcactctgt tcgaggaccg
     3901 ggagatgatc gaggagcgcc tgaagactta cgcccatctc ttcgatgaca aggtcatgaa
     3961 gcagctcaag aggaggaggt acaccggctg ggggaggctg agcaggaagc tcatcaacgg
     4021 cattcgggac aagcagtccg ggaagacgat cctcgacttc ctgaagagcg atggcttcgc
     4081 gaaccgcaat ttcatgcagc tgattcacga tgacagcctc acattcaagg aggatatcca
     4141 gaaggctcag gtgagcggcc agggggactc gctgcacgag catatcgcga acctcgctgg
     4201 ctcgccagct atcaagaagg ggattctgca gaccgtgaag gttgtggacg agctggtgaa
     4261 ggtcatgggc aggcacaagc ctgagaacat cgtcattgag atggcccggg agaatcagac
     4321 cacgcagaag ggccagaaga actcacgcga gaggatgaag aggatcgagg agggcattaa
     4381 ggagctgggg tcccagatcc tcaaggagca cccggtggag aacacgcagc tgcagaatga
     4441 gaagctctac ctgtactacc tccagaatgg ccgcgatatg tatgtggacc aggagctgga
     4501 tattaacagg ctcagcgatt acgacgtcga tgccatcgtt ccacagtcat tcctgaagga
     4561 tgactccatt gacaacaagg tcctcaccag gtcggacaag aaccggggca agtctgataa
     4621 tgttccttca gaggaggtcg ttaagaagat gaagaactac tggcgccagc tcctgaatgc
     4681 caagctgatc acgcagcgga agttcgataa cctcacaaag gctgagaggg gcgggctctc
     4741 tgagctggac aaggcgggct tcatcaagag gcagctggtc gagacacggc agatcactaa
     4801 gcacgttgcg cagattctcg actcacggat gaacactaag tacgatgaga atgacaagct
     4861 gatccgcgag gtgaaggtca tcaccctgaa gtcaaagctc gtctccgact tcaggaagga
     4921 tttccagttc tacaaggttc gggagatcaa caattaccac catgcccatg acgcgtacct
     4981 gaacgcggtg gtcggcacag ctctgatcaa gaagtaccca aagctcgaga gcgagttcgt
     5041 gtacggggac tacaaggttt acgatgtgag gaagatgatc gccaagtcgg agcaggagat
     5101 tggcaaggct accgccaagt acttcttcta ctctaacatt atgaatttct tcaagacaga
     5161 gatcactctg gccaatggcg agatccggaa gcgccccctc atcgagacga acggcgagac
     5221 gggggagatc gtgtgggaca agggcaggga tttcgcgacc gtcaggaagg ttctctccat
     5281 gccacaagtg aatatcgtca agaagacaga ggtccagact ggcgggttct ctaaggagtc
     5341 aattctgcct aagcggaaca gcgacaagct catcgcccgc aagaaggact gggatccgaa
     5401 gaagtacggc gggttcgaca gccccactgt ggcctactcg gtcctggttg tggcgaaggt
     5461 tgagaagggc aagtccaaga agctcaagag cgtgaaggag ctgctgggga tcacgattat
     5521 ggagcgctcc agcttcgaga agaacccgat cgatttcctg gaggcgaagg gctacaagga
     5581 ggtgaagaag gacctgatca ttaagctccc caagtactca ctcttcgagc tggagaacgg
     5641 caggaagcgg atgctggctt ccgctggcga gctgcagaag gggaacgagc tggctctgcc
     5701 gtccaagtat gtgaacttcc tctacctggc ctcccactac gagaagctca agggcagccc
     5761 cgaggacaac gagcagaagc agctgttcgt cgagcagcac aagcattacc tcgacgagat
     5821 cattgagcag atttccgagt tctccaagcg cgtgatcctg gccgacgcga atctggataa
     5881 ggtcctctcc gcgtacaaca agcaccgcga caagccaatc agggagcagg ctgagaatat
     5941 cattcatctc ttcaccctga cgaacctcgg cgcccctgct gctttcaagt acttcgacac
     6001 aactatcgat cgcaagaggt acacaagcac taaggaggtc ctggacgcga ccctcatcca
     6061 ccagtcgatt accggcctct acgagacgcg catcgacctg tctcagctcg ggggcgacga
     6121 attcccaaag aagaagcgga aggtggagct cagcggagga tcttccggag gatctagcgg
     6181 ctccgagaca ccaggaacat ccgaaagcgc tacaccagaa tctagcggag gctcttccgg
     6241 aggatctagg cctaccctca acatcgagga tgagtatcgc ctccacgaaa cctccaaaga
     6301 accggacgtg tccctcggca gcacatggct cagcgacttc ccacaagcgt gggccgaaac
     6361 cggcggcatg ggcctcgccg tccgccaagc cccactcatt atcccgctga aggcgacctc
     6421 cacaccggtg tccatcaagc agtacccgat gagccaagag gcgaggctcg ggattaagcc
     6481 gcacattcag cgcctcctcg atcaaggcat tctcgtgccg tgccaatccc cgtggaatac
     6541 accactcctc ccggtcaaaa agccgggcac caacgactat cgcccggtcc aagatctccg
     6601 cgaggtcaac aagcgcgtgg aagacatcca cccgaccgtc ccgaacccgt ataatctgct
     6661 ctccgggctc ccaccatccc accagtggta tacagtgctg gacctcaaag acgccttctt
     6721 ctgtctccgc ctccacccaa caagccagcc gctcttcgcc ttcgagtggc gcgacccgga
     6781 gatgggcatc tccggccaac tgacatggac acgcctcccg caaggcttca agaacagccc
     6841 gacactcttc aacgaggcgc tccataggga cctcgcggat tttcgcatcc agcatccgga
     6901 cctcatcctc ctccagtatg tggatgatct cctcctcgcc gcgacctccg agctggattg
     6961 tcaacaaggc acacgcgcgc tcctccaaac actcgggaac ctcggctatc gcgcgtccgc
     7021 gaaaaaggcc caaatctgcc agaagcaagt gaagtacctc gggtatctgc tcaaggaagg
     7081 ccaacgctgg ctcaccgaag cgcgcaaaga aacagtgatg gggcaaccga caccgaaaac
     7141 accacgccag ctgcgcgagt ttctcggcaa agccggcttc tgtcgcctct tcatcccggg
     7201 ctttgccgag atggccgcgc cactctaccc actcaccaag ccgggcacac tgtttaactg
     7261 ggggccggat cagcagaaag cctaccaaga gatcaaacaa gcgctcctca ccgccccagc
     7321 gctcgggctc ccagatctca caaagccgtt cgagctgttc gtcgatgaga agcaaggcta
     7381 cgcgaagggc gtgctcacac agaagctcgg cccgtggagg aggccagtgg cctatctctc
     7441 caaaaaactc gatccagtgg ccgccggctg gccaccgtgt ctgcgcatgg tcgccgcgat
     7501 tgccgtgctc acaaaggatg ccggcaaact cacaatgggc cagccgctgg tgatcctcgc
     7561 gccacatgcc gtggaagccc tcgtcaaaca gccgccggat aggtggctct ccaatgcgcg
     7621 catgacccat taccaagcgc tcctcctcga caccgatcgc gtccagttcg gcccagtggt
     7681 cgccctcaat ccggcgacac tgctgccact cccagaggag ggcctccaac acaactgtct
     7741 ggatattctc gcggaagcgc atggcacaag gccagacctc acagatcaac cgctcccgga
     7801 tgcggatcac acatggtata ccgacggctc ctctctgctc caagagggcc aaaggaaagc
     7861 cggcgccgcg gtgaccacag aaacagaagt gatctgggcc aaggccctcc cagccggcac
     7921 atccgcgcaa agggcggaac tcatcgcgct cacacaagcc ctcaagatgg ccgagggcaa
     7981 gaagctcaac gtctacacag actcccgcta tgccttcgcc accgcccaca ttcacggcga
     8041 aatctatagg aggcgcggct ggctcacaag cgaggggaag gagatcaaga acaaggatga
     8101 gatcctcgcg ctgctcaagg ccctctttct cccgaagcgc ctctccatca tccactgtcc
     8161 gggccaccaa aaggggcact ccgcggaagc gaggggcaat aggatggccg atcaagccgc
     8221 gcgcaaagcc gcgattaccg aaaccccaga cacatccacc ctcctcatcg aaaactcctc
     8281 cccaagcggc ggcagcccga agaagaaaag gaaggtgtga gcggccgcgg tacgctgaaa
     8341 tcaccagtct ctctctacaa atctatctct ctctattttc tccataaata atgtgtgagt
     8401 agtttcccga taagggaaat tagggttctt atagggtttc gctcatgtgt tgagcatata
     8461 agaaaccctt agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc
     8521 ctaaaaccaa aatccagtac taaaatccag atctcctaaa gtccctatag atctttgtcg
     8581 tgaatataaa ccagacacga gacgactaaa cctggagccc agacgccgtt cgaagctaga
     8641 agtaccgctt aggcaggagg ccgttaggga aaagatgcta aggcagggtt ggttacgttg
     8701 actcccccgt aggtttggtt taaatatgat gaagtggacg gaaggaagga ggaagacaag
     8761 gaaggataag gttgcaggcc ctgtgcaagg taagaagatg gaaatttgat agaggtacgc
     8821 tactatactt atactatacg ctaagggaat gcttgtattt ataccctata ccccctaata
     8881 accccttatc aatttaagaa ataatccgca taagcccccg cttaaaaatt ggtatcagag
     8941 ccatgaatag gtctatgacc aaaactcaag aggataaaac ctcaccaaaa tacgaaagag
     9001 ttcttaactc taaagataaa agatctttca agatcaaaac tagttccctc acaccggtga
     9061 cggggatcgc atgcgatatc tcgagatcta gcttggcgta atcatggtca tagctgtttc
     9121 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     9181 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     9241 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     9301 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     9361 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     9421 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     9481 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     9541 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     9601 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     9661 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     9721 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     9781 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     9841 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     9901 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     9961 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
    10021 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
    10081 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
    10141 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
    10201 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
    10261 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
    10321 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
    10381 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
    10441 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
    10501 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
    10561 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
    10621 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
    10681 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
    10741 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    10801 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    10861 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    10921 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    10981 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    11041 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    11101 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    11161 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    11221 taggggttcc gcgcacattt ccccgaaaag tgccacctgc cagtgccaag ctaattcgag
    11281 ctcggtacc
//
