LOCUS       sequence-296176-       19918 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For plant prime editing in rice plants or monocotyledons 
            protoplasts.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19918)
  AUTHORS   Lin Q, Zong Y, Xue C, Wang S, Jin S, Zhu Z, Wang Y, Anzalone AV, 
            Raguram A, Doman JL, Liu DR, Gao C
  TITLE     Prime genome editing in rice and wheat.
  JOURNAL   Nat Biotechnol. 2020 May;38(5):582-585. doi: 
            10.1038/s41587-020-0455-x. Epub 2020 Mar 16.
  PUBMED    32393904
REFERENCE   2  (bases 1 to 19918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0455-x"; journalName: "Nat Biotechnol"; date: 
            "2020-05"; volume: "38"; issue: "5"; pages: "582-585"
COMMENT     Sequence Label: pH-nCas9-PPE
FEATURES             Location/Qualifiers
     source          1..19918
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..380
                     /label=OsU3 promoter
                     /note="Oryza sativa (rice) snRNA U3 promoter"
     CDS             660..1451
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_RNA        1603..1678
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        1982..3973
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             3984..4004
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(4005..6518,6519..6521,6522..8105)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             8018..8026
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             8112..8132
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             10284..10304
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(10963..10979)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10963..10979)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10976..10998)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10987..11003
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11011..11017,11018..11035,11036..11041))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11056..11077
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        11268..11944
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     11881..11901
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             12011..13036
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    13077..13251
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    13329..13353
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             13778..14572
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      14659..15247
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15148..15167
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     15401..15418
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    15433..15573
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(15499..15518)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     15659..15681
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      15917..16111
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(16177..17250)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(17679..18308)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    19609..19633
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     19821..19843
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     19835..19852
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     19836..19852
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aaggaatctt taaacatacg aacagatcac ttaaagttct tctgaagcaa cttaaagtta
       61 tcaggcatgc atggatcttg gaggaatcag atgtgcagtc agggaccata gcacaagaca
      121 ggcgtcttct actggtgcta ccagcaaatg ctggaagccg ggaacactgg gtacgttgga
      181 aaccacgtga tgtgaagaag taagataaac tgtaggagaa aagcatttcg tagtgggcca
      241 tgaagccttt caggacatgt attgcagtat gggccggccc attacgcaat tggacgacaa
      301 caaagactag tattagtacc acctcggcta tccacataga tcaaagctga tttaaaagag
      361 ttgtgcagat gatccgtggc gtgagaccaa cccagtggac ataagcctgt tcggttcgta
      421 agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca
      481 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac
      541 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt
      601 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca
      661 tgggggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg
      721 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
      781 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa
      841 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
      901 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
      961 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta
     1021 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac
     1081 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg
     1141 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg
     1201 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca
     1261 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc
     1321 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct
     1381 cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag
     1441 tcggcaaata atgtctagct agaaattcgt tcaagccgac gccgcttcgc ggcgcggctt
     1501 aactcaagcg ttagatgcac taagcacata attgctcaca gccaaactat caggtcaagt
     1561 ctgcttttat tatttttaag cgtgcataat aagccggtct cggttttaga gctagaaata
     1621 gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt
     1681 ttttttttcg ttttgcattg agttttctcc gtcgcatgtt tgcagtttta ttttccgttt
     1741 tgcattgaaa tttctccgtc tcatgtttgc agcgtgttca aaaagtacgc agctgtattt
     1801 cacttattta cggcgccaca ttttcatgcc gtttgtgcca actatcccga gctagtgaat
     1861 acagcttggc ttcacacaac actggtgacc cgctgacctg ctcgtacctc gtaccgtcgt
     1921 acggcacagc atttggaatt aaagggtgtg atcgatactg cttgctgcta agcttgcatg
     1981 cctgcagtgc agcgtgaccc ggtcgtgccc ctctctagag ataatgagca ttgcatgtct
     2041 aagttataaa aaattaccac atattttttt tgtcacactt gtttgaagtg cagtttatct
     2101 atctttatac atatatttaa actttactct acgaataata taatctatag tactacaata
     2161 atatcagtgt tttagagaat catataaatg aacagttaga catggtctaa aggacaattg
     2221 agtattttga caacaggact ctacagtttt atctttttag tgtgcatgtg ttctcctttt
     2281 tttttgcaaa tagcttcacc tatataatac ttcatccatt ttattagtac atccatttag
     2341 ggtttagggt taatggtttt tatagactaa tttttttagt acatctattt tattctattt
     2401 tagcctctaa attaagaaaa ctaaaactct attttagttt ttttatttaa taatttagat
     2461 ataaaataga ataaaataaa gtgactaaaa attaaacaaa taccctttaa gaaattaaaa
     2521 aaactaagga aacatttttc ttgtttcgag tagataatgc cagcctgtta aacgccgtcg
     2581 acgagtctaa cggacaccaa ccagcgaacc agcagcgtcg cgtcgggcca agcgaagcag
     2641 acggcacggc atctctgtcg ctgcctctgg acccctctcg agagttccgc tccaccgttg
     2701 gacttgctcc gctgtcggca tccagaaatt gcgtggcgga gcggcagacg tgagccggca
     2761 cggcaggcgg cctcctcctc ctctcacggc accggcagct acgggggatt cctttcccac
     2821 cgctccttcg ctttcccttc ctcgcccgcc gtaataaata gacaccccct ccacaccctc
     2881 tttccccaac ctcgtgttgt tcggagcgca cacacacaca accagatctc ccccaaatcc
     2941 acccgtcggc acctccgctt caaggtacgc cgctcgtcct cccccccccc cctctctacc
     3001 ttctctagat cggcgttccg gtccatggtt agggcccggt agttctactt ctgttcatgt
     3061 ttgtgttaga tccgtgtttg tgttagatcc gtgctgctag cgttcgtaca cggatgcgac
     3121 ctgtacgtca gacacgttct gattgctaac ttgccagtgt ttctctttgg ggaatcctgg
     3181 gatggctcta gccgttccgc agacgggatc gatttcatga ttttttttgt ttcgttgcat
     3241 agggtttggt ttgccctttt cctttatttc aatatatgcc gtgcacttgt ttgtcgggtc
     3301 atcttttcat gctttttttt gtcttggttg tgatgatgtg gtctggttgg gcggtcgttc
     3361 tagatcggag tagaattctg tttcaaacta cctggtggat ttattaattt tggatctgta
     3421 tgtgtgtgcc atacatattc atagttacga attgaagatg atggatggaa atatcgatct
     3481 aggataggta tacatgttga tgcgggtttt actgatgcat atacagagat gctttttgtt
     3541 cgcttggttg tgatgatgtg gtgtggttgg gcggtcgttc attcgttcta gatcggagta
     3601 gaatactgtt tcaaactacc tggtgtattt attaattttg gaactgtatg tgtgtgtcat
     3661 acatcttcat agttacgagt ttaagatgga tggaaatatc gatctaggat aggtatacat
     3721 gttgatgtgg gttttactga tgcatataca tgatggcata tgcagcatct attcatatgc
     3781 tctaaccttg agtacctatc tattataata aacaagtatg ttttataatt attttgatct
     3841 tgatatactt ggatgatggc atatgcagca gctatatgtg gattttttta gccctgcctt
     3901 catacgctat ttatttgctt ggtactgttt cttttgtcga tgctcaccct gttgtttggt
     3961 gttacttctg cagccctagg atgcctaaga aaaagagaaa agtggacaag aagtactcga
     4021 tcggcctcga tattgggact aactctgttg gctgggccgt gatcaccgac gagtacaagg
     4081 tgccctcaaa gaagttcaag gtcctgggca acaccgatcg gcattccatc aagaagaatc
     4141 tcattggcgc tctcctgttc gacagcggcg agacggctga ggctacgcgg ctcaagcgca
     4201 ccgcccgcag gcggtacacg cgcaggaaga atcgcatctg ctacctgcag gagattttct
     4261 ccaacgagat ggcgaaggtt gacgattctt tcttccacag gctggaggag tcattcctcg
     4321 tggaggagga taagaagcac gagcggcatc caatcttcgg caacattgtc gacgaggttg
     4381 cctaccacga gaagtaccct acgatctacc atctgcggaa gaagctcgtg gactccacag
     4441 ataaggcgga cctccgcctg atctacctcg ctctggccca catgattaag ttcaggggcc
     4501 atttcctgat cgagggggat ctcaacccgg acaatagcga tgttgacaag ctgttcatcc
     4561 agctcgtgca gacgtacaac cagctcttcg aggagaaccc cattaatgcg tcaggcgtcg
     4621 acgcgaaggc tatcctgtcc gctaggctct cgaagtctcg gcgcctcgag aacctgatcg
     4681 cccagctgcc gggcgagaag aagaacggcc tgttcgggaa tctcattgcg ctcagcctgg
     4741 ggctcacgcc caacttcaag tcgaatttcg atctcgctga ggacgccaag ctgcagctct
     4801 ccaaggacac atacgacgat gacctggata acctcctggc ccagatcggc gatcagtacg
     4861 cggacctgtt cctcgctgcc aagaatctgt cggacgccat cctcctgtct gatattctca
     4921 gggtgaacac cgagattacg aaggctccgc tctcagcctc catgatcaag cgctacgacg
     4981 agcaccatca ggatctgacc ctcctgaagg cgctggtcag gcagcagctc cccgagaagt
     5041 acaaggagat cttcttcgat cagtcgaaga acggctacgc tgggtacatt gacggcgggg
     5101 cctctcagga ggagttctac aagttcatca agccgattct ggagaagatg gacggcacgg
     5161 aggagctgct ggtgaagctc aatcgcgagg acctcctgag gaagcagcgg acattcgata
     5221 acggcagcat cccacaccag attcatctcg gggagctgca cgctatcctg aggaggcagg
     5281 aggacttcta ccctttcctc aaggataacc gcgagaagat cgagaagatt ctgactttca
     5341 ggatcccgta ctacgtcggc ccactcgcta ggggcaactc ccgcttcgct tggatgaccc
     5401 gcaagtcaga ggagacgatc acgccgtgga acttcgagga ggtggtcgac aagggcgcta
     5461 gcgctcagtc gttcatcgag aggatgacga atttcgacaa gaacctgcca aatgagaagg
     5521 tgctccctaa gcactcgctc ctgtacgagt acttcacagt ctacaacgag ctgactaagg
     5581 tgaagtatgt gaccgagggc atgaggaagc cggctttcct gtctggggag cagaagaagg
     5641 ccatcgtgga cctcctgttc aagaccaacc ggaaggtcac ggttaagcag ctcaaggagg
     5701 actacttcaa gaagattgag tgcttcgatt cggtcgagat ctctggcgtt gaggaccgct
     5761 tcaacgcctc cctggggacc taccacgatc tcctgaagat cattaaggat aaggacttcc
     5821 tggacaacga ggagaatgag gatatcctcg aggacattgt gctgacactc actctgttcg
     5881 aggaccggga gatgatcgag gagcgcctga agacttacgc ccatctcttc gatgacaagg
     5941 tcatgaagca gctcaagagg aggaggtaca ccggctgggg gaggctgagc aggaagctca
     6001 tcaacggcat tcgggacaag cagtccggga agacgatcct cgacttcctg aagagcgatg
     6061 gcttcgcgaa ccgcaatttc atgcagctga ttcacgatga cagcctcaca ttcaaggagg
     6121 atatccagaa ggctcaggtg agcggccagg gggactcgct gcacgagcat atcgcgaacc
     6181 tcgctggctc gccagctatc aagaagggga ttctgcagac cgtgaaggtt gtggacgagc
     6241 tggtgaaggt catgggcagg cacaagcctg agaacatcgt cattgagatg gcccgggaga
     6301 atcagaccac gcagaagggc cagaagaact cacgcgagag gatgaagagg atcgaggagg
     6361 gcattaagga gctggggtcc cagatcctca aggagcaccc ggtggagaac acgcagctgc
     6421 agaatgagaa gctctacctg tactacctcc agaatggccg cgatatgtat gtggaccagg
     6481 agctggatat taacaggctc agcgattacg acgtcgatgc catcgttcca cagtcattcc
     6541 tgaaggatga ctccattgac aacaaggtcc tcaccaggtc ggacaagaac cggggcaagt
     6601 ctgataatgt tccttcagag gaggtcgtta agaagatgaa gaactactgg cgccagctcc
     6661 tgaatgccaa gctgatcacg cagcggaagt tcgataacct cacaaaggct gagaggggcg
     6721 ggctctctga gctggacaag gcgggcttca tcaagaggca gctggtcgag acacggcaga
     6781 tcactaagca cgttgcgcag attctcgact cacggatgaa cactaagtac gatgagaatg
     6841 acaagctgat ccgcgaggtg aaggtcatca ccctgaagtc aaagctcgtc tccgacttca
     6901 ggaaggattt ccagttctac aaggttcggg agatcaacaa ttaccaccat gcccatgacg
     6961 cgtacctgaa cgcggtggtc ggcacagctc tgatcaagaa gtacccaaag ctcgagagcg
     7021 agttcgtgta cggggactac aaggtttacg atgtgaggaa gatgatcgcc aagtcggagc
     7081 aggagattgg caaggctacc gccaagtact tcttctactc taacattatg aatttcttca
     7141 agacagagat cactctggcc aatggcgaga tccggaagcg ccccctcatc gagacgaacg
     7201 gcgagacggg ggagatcgtg tgggacaagg gcagggattt cgcgaccgtc aggaaggttc
     7261 tctccatgcc acaagtgaat atcgtcaaga agacagaggt ccagactggc gggttctcta
     7321 aggagtcaat tctgcctaag cggaacagcg acaagctcat cgcccgcaag aaggactggg
     7381 atccgaagaa gtacggcggg ttcgacagcc ccactgtggc ctactcggtc ctggttgtgg
     7441 cgaaggttga gaagggcaag tccaagaagc tcaagagcgt gaaggagctg ctggggatca
     7501 cgattatgga gcgctccagc ttcgagaaga acccgatcga tttcctggag gcgaagggct
     7561 acaaggaggt gaagaaggac ctgatcatta agctccccaa gtactcactc ttcgagctgg
     7621 agaacggcag gaagcggatg ctggcttccg ctggcgagct gcagaagggg aacgagctgg
     7681 ctctgccgtc caagtatgtg aacttcctct acctggcctc ccactacgag aagctcaagg
     7741 gcagccccga ggacaacgag cagaagcagc tgttcgtcga gcagcacaag cattacctcg
     7801 acgagatcat tgagcagatt tccgagttct ccaagcgcgt gatcctggcc gacgcgaatc
     7861 tggataaggt cctctccgcg tacaacaagc accgcgacaa gccaatcagg gagcaggctg
     7921 agaatatcat tcatctcttc accctgacga acctcggcgc ccctgctgct ttcaagtact
     7981 tcgacacaac tatcgatcgc aagaggtaca caagcactaa ggaggtcctg gacgcgaccc
     8041 tcatccacca gtcgattacc ggcctctacg agacgcgcat cgacctgtct cagctcgggg
     8101 gcgacgaatt cccaaagaag aagcggaagg tggagctcag cggaggatct tccggaggat
     8161 ctagcggctc cgagacacca ggaacatccg aaagcgctac accagaatct agcggaggct
     8221 cttccggagg atctaggcct accctcaaca tcgaggatga gtatcgcctc cacgaaacct
     8281 ccaaagaacc ggacgtgtcc ctcggcagca catggctcag cgacttccca caagcgtggg
     8341 ccgaaaccgg cggcatgggc ctcgccgtcc gccaagcccc actcattatc ccgctgaagg
     8401 cgacctccac accggtgtcc atcaagcagt acccgatgag ccaagaggcg aggctcggga
     8461 ttaagccgca cattcagcgc ctcctcgatc aaggcattct cgtgccgtgc caatccccgt
     8521 ggaatacacc actcctcccg gtcaaaaagc cgggcaccaa cgactatcgc ccggtccaag
     8581 atctccgcga ggtcaacaag cgcgtggaag acatccaccc gaccgtcccg aacccgtata
     8641 atctgctctc cgggctccca ccatcccacc agtggtatac agtgctggac ctcaaagacg
     8701 ccttcttctg tctccgcctc cacccaacaa gccagccgct cttcgccttc gagtggcgcg
     8761 acccggagat gggcatctcc ggccaactga catggacacg cctcccgcaa ggcttcaaga
     8821 acagcccgac actcttcaac gaggcgctcc atagggacct cgcggatttt cgcatccagc
     8881 atccggacct catcctcctc cagtatgtgg atgatctcct cctcgccgcg acctccgagc
     8941 tggattgtca acaaggcaca cgcgcgctcc tccaaacact cgggaacctc ggctatcgcg
     9001 cgtccgcgaa aaaggcccaa atctgccaga agcaagtgaa gtacctcggg tatctgctca
     9061 aggaaggcca acgctggctc accgaagcgc gcaaagaaac agtgatgggg caaccgacac
     9121 cgaaaacacc acgccagctg cgcgagtttc tcggcaaagc cggcttctgt cgcctcttca
     9181 tcccgggctt tgccgagatg gccgcgccac tctacccact caccaagccg ggcacactgt
     9241 ttaactgggg gccggatcag cagaaagcct accaagagat caaacaagcg ctcctcaccg
     9301 ccccagcgct cgggctccca gatctcacaa agccgttcga gctgttcgtc gatgagaagc
     9361 aaggctacgc gaagggcgtg ctcacacaga agctcggccc gtggaggagg ccagtggcct
     9421 atctctccaa aaaactcgat ccagtggccg ccggctggcc accgtgtctg cgcatggtcg
     9481 ccgcgattgc cgtgctcaca aaggatgccg gcaaactcac aatgggccag ccgctggtga
     9541 tcctcgcgcc acatgccgtg gaagccctcg tcaaacagcc gccggatagg tggctctcca
     9601 atgcgcgcat gacccattac caagcgctcc tcctcgacac cgatcgcgtc cagttcggcc
     9661 cagtggtcgc cctcaatccg gcgacactgc tgccactccc agaggagggc ctccaacaca
     9721 actgtctgga tattctcgcg gaagcgcatg gcacaaggcc agacctcaca gatcaaccgc
     9781 tcccggatgc ggatcacaca tggtataccg acggctcctc tctgctccaa gagggccaaa
     9841 ggaaagccgg cgccgcggtg accacagaaa cagaagtgat ctgggccaag gccctcccag
     9901 ccggcacatc cgcgcaaagg gcggaactca tcgcgctcac acaagccctc aagatggccg
     9961 agggcaagaa gctcaacgtc tacacagact cccgctatgc cttcgccacc gcccacattc
    10021 acggcgaaat ctataggagg cgcggctggc tcacaagcga ggggaaggag atcaagaaca
    10081 aggatgagat cctcgcgctg ctcaaggccc tctttctccc gaagcgcctc tccatcatcc
    10141 actgtccggg ccaccaaaag gggcactccg cggaagcgag gggcaatagg atggccgatc
    10201 aagccgcgcg caaagccgcg attaccgaaa ccccagacac atccaccctc ctcatcgaaa
    10261 actcctcccc aagcggcggc agcccgaaga agaaaaggaa ggtgtgagag ctcagagctt
    10321 tcgttcgtat catcggtttc gacaacgttc gtcaagttca atgcatcagt ttcattgcgc
    10381 acacaccaga atcctactga gtttgagtat tatggcattg ggaaaactgt ttttcttgta
    10441 ccatttgttg tgcttgtaat ttactgtgtt ttttattcgg ttttcgctat cgaactgtga
    10501 aatggaaatg gatggagaag agttaatgaa tgatatggtc cttttgttca ttctcaaatt
    10561 aatattattt gttttttctc ttatttgttg tgtgttgaat ttgaaattat aagagatatg
    10621 caaacatttt gttttgagta aaaatgtgtc aaatcgtggc ctctaatgac cgaagttaat
    10681 atgaggagta aaacacttgt agttgtacca ttatgcttat tcactaggca acaaatatat
    10741 tttcagacct agaaaagctg caaatgttac tgaatacaag tatgtcctct tgtgttttag
    10801 acatttatga actttccttt atgtaatttt ccagaatcct tgtcagattc taatcattgc
    10861 tttataatta tagttatact catggatttg tagttgagta tgaaaatatt ttttaatgca
    10921 ttttatgact tgccaattga ttgacaacga attcgtaatc atgtcatagc tgtttcctgt
    10981 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
    11041 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
    11101 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
    11161 aggcggtttg cgtattggct agagcagctt gccaacatgg tggagcacga cactctcgtc
    11221 tactccaaga atatcaaaga tacagtctca gaagaccaaa gggctattga gacttttcaa
    11281 caaagggtaa tatcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc
    11341 aaaaggacag tagaaaagga aggtggcacc tacaaatgcc atcattgcga taaaggaaag
    11401 gctatcgttc aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg
    11461 agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgaa
    11521 catggtggag cacgacactc tcgtctactc caagaatatc aaagatacag tctcagaaga
    11581 ccaaagggct attgagactt ttcaacaaag ggtaatatcg ggaaacctcc tcggattcca
    11641 ttgcccagct atctgtcact tcatcaaaag gacagtagaa aaggaaggtg gcacctacaa
    11701 atgccatcat tgcgataaag gaaaggctat cgttcaagat gcctctgccg acagtggtcc
    11761 caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc
    11821 ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg cacaatccca
    11881 ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg agaggacacg
    11941 ctgaaatcac cagtctctct ctacaaatct atctctctcg agctttcgca gatccggggg
    12001 gcaatgagat atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga
    12061 aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt
    12121 cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt
    12181 ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt
    12241 gcttgacatt ggggagttta gcgagagcct gacctattgc atctcccgcc gttcacaggg
    12301 tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctacaac cggtcgcgga
    12361 ggctatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg
    12421 accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc
    12481 ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc
    12541 tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc
    12601 ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg
    12661 gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc
    12721 gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc
    12781 aggatcgcca cgactccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag
    12841 cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt
    12901 ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg
    12961 gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc
    13021 gagggcaaag aaatagagta gatgccgacc gggatctgtc gatcgacaag ctcgagtttc
    13081 tccataataa tgtgtgagta gttcccagat aagggaatta gggttcctat agggtttcgc
    13141 tcatgtgttg agcatataag aaacccttag tatgtatttg tatttgtaaa atacttctat
    13201 caataaaatt tctaattcct aaaaccaaaa tccagtacta aaatccagat cccccgaatt
    13261 aattcggcgt taattcagta cattaaaaac gtccgcaatg tgttattaag ttgtctaagc
    13321 gtcaatttgt ttacaccaca atatatcctg ccaccagcca gccaacagct ccccgaccgg
    13381 cagctcggca caaaatcacc actcgataca ggcagcccat cagtccggga cggcgtcagc
    13441 gggagagccg ttgtaaggcg gcagactttg ctcatgttac cgatgctatt cggaagaacg
    13501 gcaactaagc tgccgggttt gaaacacgga tgatctcgcg gagggtagca tgttgattgt
    13561 aacgatgaca gagcgttgct gcctgtgatc accgcggttt caaaatcggc tccgtcgata
    13621 ctatgttata cgccaacttt gaaaacaact ttgaaaaagc tgttttctgg tatttaaggt
    13681 tttagaatgc aaggaacagt gaattggagt tcgtcttgtt ataattagct tcttggggta
    13741 tctttaaata ctgtagaaaa gaggaaggaa ataataaatg gctaaaatga gaatatcacc
    13801 ggaattgaaa aaactgatcg aaaaataccg ctgcgtaaaa gatacggaag gaatgtctcc
    13861 tgctaaggta tataagctgg tgggagaaaa tgaaaaccta tatttaaaaa tgacggacag
    13921 ccggtataaa gggaccacct atgatgtgga acgggaaaag gacatgatgc tatggctgga
    13981 aggaaagctg cctgttccaa aggtcctgca ctttgaacgg catgatggct ggagcaatct
    14041 gctcatgagt gaggccgatg gcgtcctttg ctcggaagag tatgaagatg aacaaagccc
    14101 tgaaaagatt atcgagctgt atgcggagtg catcaggctc tttcactcca tcgacatatc
    14161 ggattgtccc tatacgaata gcttagacag ccgcttagcc gaattggatt acttactgaa
    14221 taacgatctg gccgatgtgg attgcgaaaa ctgggaagaa gacactccat ttaaagatcc
    14281 gcgcgagctg tatgattttt taaagacgga aaagcccgaa gaggaacttg tcttttccca
    14341 cggcgacctg ggagacagca acatctttgt gaaagatggc aaagtaagtg gctttattga
    14401 tcttgggaga agcggcaggg cggacaagtg gtatgacatt gccttctgcg tccggtcgat
    14461 cagggaggat atcggggaag aacagtatgt cgagctattt tttgacttac tggggatcaa
    14521 gcctgattgg gagaaaataa aatattatat tttactggat gaattgtttt agtacctaga
    14581 atgcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
    14641 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
    14701 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
    14761 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
    14821 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
    14881 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
    14941 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
    15001 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
    15061 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
    15121 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
    15181 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
    15241 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
    15301 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
    15361 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
    15421 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
    15481 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact
    15541 ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac
    15601 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
    15661 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagggtgcc
    15721 ttgatgtggg cgccggcggt cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt
    15781 gcctggccgt aggccagcca tttttgagcg gccagcggcc gcgataggcc gacgcgaagc
    15841 ggcggggcgt agggagcgca gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt
    15901 gcgctggcca gacagttatg cacaggccag gcgggtttta agagttttaa taagttttaa
    15961 agagttttag gcggaaaaat cgcctttttt ctcttttata tcagtcactt acatgtgtga
    16021 ccggttccca atgtacggct ttgggttccc aatgtacggg ttccggttcc caatgtacgg
    16081 ctttgggttc ccaatgtacg tgctatccac aggaaacaga ccttttcgac ctttttcccc
    16141 tgctagggca atttgcccta gcatctgctc cgtacattag gaaccggcgg atgcttcgcc
    16201 ctcgatcagg ttgcggtagc gcatgactag gatcgggcca gcctgccccg cctcctcctt
    16261 caaatcgtac tccggcaggt catttgaccc gatcagcttg cgcacggtga aacagaactt
    16321 cttgaactct ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc atctggcttc
    16381 tgccttgcct gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat
    16441 caaaaagtaa tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg
    16501 gtacatccaa tcagctagct cgatctcgat gtactccggc cgcccggttt cgctctttac
    16561 gatcttgtag cggctaatca aggcttcacc ctcggatacc gtcaccaggc ggccgttctt
    16621 ggccttcttc gtacgctgca tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac
    16681 caggtcgtct ttctgctttc cgccatcggc tcgccggcag aacttgagta cgtccgcaac
    16741 gtgtggacgg aacacgcggc cgggcttgtc tcccttccct tcccggtatc ggttcatgga
    16801 ttcggttaga tgggaaaccg ccatcagtac caggtcgtaa tcccacacac tggccatgcc
    16861 ggccggccct gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc
    16921 agctcgtcgg tcacgcttcg acagacggaa aacggccacg tccatgatgc tgcgactatc
    16981 gcgggtgccc acgtcataga gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg
    17041 cggcttccta atcgacggcg caccggctgc cggcggttgc cgggattctt tgcggattcg
    17101 atcagcggcc gcttgccacg attcaccggg gcgtgcttct gcctcgatgc gttgccgctg
    17161 ggcggcctgc gcggccttca acttctccac caggtcatca cccagcgccg cgccgatttg
    17221 taccgggccg gatggtttgc gaccgctcac gccgattcct cgggcttggg ggttccagtg
    17281 ccattgcagg gccggcaggc aacccagccg cttacgcctg gccaaccgcc cgttcctcca
    17341 cacatggggc attccacggc gtcggtgcct ggttgttctt gattttccat gccgcctcct
    17401 ttagccgcta aaattcatct actcatttat tcatttgctc atttactctg gtagctgcgc
    17461 gatgtattca gatagcagct cggtaatggt cttgccttgg cgtaccgcgt acatcttcag
    17521 cttggtgtga tcctccgccg gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc
    17581 caggctggcc aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt
    17641 gtttgtgctt ttgctcattt tctctttacc tcattaactc aaatgagttt tgatttaatt
    17701 tcagcggcca gcgcctggac ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg
    17761 gttgtgccgg cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga
    17821 atgggcagct cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg
    17881 atcgcccgcg acacgacaaa ggccgcttgt agccttccat ccgtgacctc aatgcgctgc
    17941 ttaaccagct ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga
    18001 atcagcacga agtcggctgc cttgatcgcg gacacagcca agtccgccgc ctggggcgct
    18061 ccgtcgatca ctacgaagtc gcgccggccg atggccttca cgtcgcggtc aatcgtcggg
    18121 cggtcgatgc cgacaacggt tagcggttga tcttcccgca cggccgccca atcgcgggca
    18181 ctgccctggg gatcggaatc gactaacaga acatcggccc cggcgagttg cagggcgcgg
    18241 gctagatggg ttgcgatggt cgtcttgcct gacccgcctt tctggttaag tacagcgata
    18301 accttcatgc gttccccttg cgtatttgtt tatttactca tcgcatcata tacgcagcga
    18361 ccgcatgacg caagctgttt tactcaaata cacatcacct ttttagacgg cggcgctcgg
    18421 tttcttcagc ggccaagctg gccggccagg ccgccagctt ggcatcagac aaaccggcca
    18481 ggatttcatg cagccgcacg gttgagacgt gcgcgggcgg ctcgaacacg tacccggccg
    18541 cgatcatctc cgcctcgatc tcttcggtaa tgaaaaacgg ttcgtcctgg ccgtcctggt
    18601 gcggtttcat gcttgttcct cttggcgttc attctcggcg gccgccaggg cgtcggcctc
    18661 ggtcaatgcg tcctcacgga aggcaccgcg ccgcctggcc tcggtgggcg tcacttcctc
    18721 gctgcgctca agtgcgcggt acagggtcga gcgatgcacg ccaagcagtg cagccgcctc
    18781 tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg tgcgcgatct gtgccggggt
    18841 gagggtaggg cgggggccaa acttcacgcc tcgggccttg gcggcctcgc gcccgctccg
    18901 ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg ccggcgaaca cggtcaacac
    18961 catgcggccg gccggcgtgg tggtgtcggc ccacggctct gccaggctac gcaggcccgc
    19021 gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg cgggtgctgc gggccaggcg
    19081 gtctagcctg gtcactgtca caacgtcgcc agggcgtagg tggtcaagca tcctggccag
    19141 ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa aacagcttgg tgcagccggc
    19201 cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg tcggtgctga cgcgggcata
    19261 gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa tgtctccggt tctagtcgca
    19321 agtattctac tttatgcgac taaaacacgc gacaagaaaa cgccaggaaa agggcagggc
    19381 ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg ttttcagaag acggctgcac
    19441 tgaacgtcag aagccgactg cactatagca gcggaggggt tggatcaaag tactttgatc
    19501 ccgaggggaa ccctgtggtt ggcatgcaca tacaaatgga cgaacggata aaccttttca
    19561 cgccctttta aatatccgat tattctaata aacgctcttt tctcttaggt ttacccgcca
    19621 atatatcctg tcaaacactg atagtttaaa ctgaaggcgg gaaacgacaa tctgatccaa
    19681 gctcaagctg ctctagcatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
    19741 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
    19801 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct
    19861 tagtaattca tccaggtctc caagttctag gattttcaga actgcaactt attttatc
//
