LOCUS       sequence-296189-       21257 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA encoding Fungal Bioluminescent Pathway Components, complete 
            pathway, without recycling gene CPH.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 21257)
  AUTHORS   Khakhar A, Starker CG, Chamness JC, Lee N, Stokke S, Wang C, Swanson
            R, Rizvi F, Imaizumi T, Voytas DF
  TITLE     Building customizable auto-luminescent luciferase-based reporters in
            plants.
  JOURNAL   Elife. 2020 Mar 25;9. pii: 52786. doi: 10.7554/eLife.52786.
  PUBMED    32209230
REFERENCE   2  (bases 1 to 21257)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-03-25"; volume: "9. pii: 52786. doi"; pages: " 
            10.7554/eLife.52786"
COMMENT     Sequence Label: P303-FBP_2
FEATURES             Location/Qualifiers
     source          1..21257
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(5..22)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5..21)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14..36)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    224..248
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1549..2178
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2607..3680
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3746..3940
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(4176..4198)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4284..4424
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4339..4358
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4439..4456)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4610..5198)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4690..4709)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5285..6079)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    6504..6528
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    6606..6780
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(6837..7634)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6948..6967)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7558..7577
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(7697..8373)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    8564..8585
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8600..8605,8606..8623,8624..8630)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8638..8654
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8643..8665
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8662..8678
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8662..8678
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        8806..9151
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      10302..10502
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     terminator      12279..12986
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     terminator      18592..18840
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     promoter        19362..19707
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      20529..21236
                     /label=OCS terminator
                     /note="octopine synthase terminator"
ORIGIN
        1 tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
       61 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
      121 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ctagagcagc ttgagcttgg
      181 atcagattgt cgtttcccgc cttcagttta aactatcagt gtttgacagg atatattggc
      241 gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa agggcgtgaa
      301 aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc cctcgggatc
      361 aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc
      421 agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc
      481 tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg
      541 actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg
      601 cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg
      661 gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg
      721 ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc
      781 tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg
      841 gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt
      901 tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga
      961 gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc
     1021 cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg
     1081 cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg
     1141 aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg
     1201 ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca
     1261 ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc
     1321 cgggtacgtg ttcgagccgc ccgcgcacgg ctcaaccgtg cggctgcatg aaatcctggc
     1381 cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga
     1441 gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc
     1501 tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc
     1561 gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc
     1621 gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc
     1681 cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg
     1741 acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg
     1801 ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg
     1861 gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag
     1921 cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa
     1981 ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt
     2041 gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt
     2101 cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt
     2161 aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc
     2221 taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag
     2281 acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga
     2341 agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc
     2401 agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga
     2461 ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga
     2521 ggaacgggcg gttggccagg cgtaagcggc tgggttgcct gccggccctg caatggcact
     2581 ggaaccccca agcccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa
     2641 tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc
     2701 ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa
     2761 tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca
     2821 agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata
     2881 gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg
     2941 aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca
     3001 tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca
     3061 tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg
     3121 cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag
     3181 aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga
     3241 acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa
     3301 agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg
     3361 agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg
     3421 atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag
     3481 ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt
     3541 tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg
     3601 aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg
     3661 aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg
     3721 aaaaaggtcg aaaaggcctc tttcctgtgg atagcacgta cattgggaac ccaaagccgt
     3781 acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac
     3841 acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa
     3901 aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag
     3961 cccaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt
     4021 cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc
     4081 taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggca
     4141 ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggaa
     4201 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     4261 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
     4321 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     4381 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggccctct tccgcttcct
     4441 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     4501 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     4561 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     4621 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     4681 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     4741 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     4801 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     4861 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     4921 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     4981 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     5041 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     5101 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     5161 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     5221 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgcattcta
     5281 ggtactaaaa caattcatcc agtaaaatat aatattttat tttctcccaa tcaggcttga
     5341 tccccagtaa gtcaaaaaat agctcgacat actgttcttc cccgatatcc tccctgatcg
     5401 accggacgca gaaggcaatg tcataccact tgtccgccct gccgcttctc ccaagatcaa
     5461 taaagccact tactttgcca tctttcacaa agatgttgct gtctcccagg tcgccgtggg
     5521 aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa atcatacagc tcgcgcggat
     5581 ctttaaatgg agtgtcttct tcccagtttt cgcaatccac atcggccaga tcgttattca
     5641 gtaagtaatc caattcggct aagcggctgt ctaagctatt cgtataggga caatccgata
     5701 tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag ctcgataatc ttttcagggc
     5761 tttgttcatc ttcatactct tccgagcaaa ggacgccatc ggcctcactc atgagcagat
     5821 tgctccagcc atcatgccgt tcaaagtgca ggacctttgg aacaggcagc tttccttcca
     5881 gccatagcat catgtccttt tcccgttcca catcataggt ggtcccttta taccggctgt
     5941 ccgtcatttt taaatatagg ttttcatttt ctcccaccag cttatatacc ttagcaggag
     6001 acattccttc cgtatctttt acgcagcggt atttttcgat cagttttttc aattccggtg
     6061 atattctcat tttagccatt tattatttcc ttcctctttt ctacagtatt taaagatacc
     6121 ccaagaagct aattataaca agacgaactc caattcactg ttccttgcat tctaaaacct
     6181 taaataccag aaaacagctt tttcaaagtt gttttcaaag ttggcgtata acatagtatc
     6241 gacggagccg attttgaaac cgcggtgatc acaggcagca acgctctgtc atcgttacaa
     6301 tcaacatgct accctccgcg agatcatccg tgtttcaaac ccggcagctt agttgccgtt
     6361 cttccgaata gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc tctcccgctg
     6421 acgccgtccc ggactgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg
     6481 tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaacaa attgacgctt
     6541 agacaactta ataacacatt gcggacgttt ttaatgtact gaattaacgc cgaattaatt
     6601 cgggggatct ggattttagt actggatttt ggttttagga attagaaatt ttattgatag
     6661 aagtatttta caaatacaaa tacatactaa gggtttctta tatgctcaac acatgagcga
     6721 aaccctatag gaaccctaat tcccttatct gggaactact cacacattat tatggagaaa
     6781 ctcgagcttg tcgatcgact ctagctagag gatcgatccg aaccccagag tcccgctcag
     6841 aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg
     6901 taaagcacga ggaagcggtc agcccattcg ccgccaagtt cttcagcaat atcacgggta
     6961 gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca
     7021 gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg tgtcacgacg
     7081 agatcctcgc cgtcgggcat gcgcgccttg agcctggcga acagttcggc tggcgcgagc
     7141 ccctgatgtt cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt
     7201 gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta
     7261 tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat
     7321 gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg
     7381 acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct
     7441 gcctcgtcct ggagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg
     7501 cgcccctgcg ctgacagccg aaacacggcg gcatcagagc agccgattgt ctgttgtgcc
     7561 cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct
     7621 tgttcaatcc ccatggtcga tcgacagatc tgcgaaagct cgagagagat agatttgtag
     7681 agagagactg gtgatttcag cgtgtcctct ccaaatgaaa tgaacttcct tatatagagg
     7741 aagggtcttg cgaaggatag tgggattgtg cgtcatccct tacgtcagtg gagatatcac
     7801 atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg
     7861 tgggtggggg tccatctttg ggaccactgt cggcagaggc atcttgaacg atagcctttc
     7921 ctttatcgca atgatggcat ttgtaggtgc caccttcctt ttctactgtc cttttgatga
     7981 agtgacagat agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa
     8041 aagtctcaat agccctttgg tcttctgaga ctgtatcttt gatattcttg gagtagacga
     8101 gagtgtcgtg ctccaccatg ttcacatcaa tccacttgct ttgaagacgt ggttggaacg
     8161 tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca
     8221 gaggcatctt gaacgatagc ctttccttta tcgcaatgat ggcatttgta ggtgccacct
     8281 tccttttcta ctgtcctttt gatgaagtga cagatagctg ggcaatggaa tccgaggagg
     8341 tttcccgata ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc tgagactgta
     8401 tctttgatat tcttggagta gacgagagtg tcgtgctcca ccatgttggc aagctgctct
     8461 agccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     8521 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     8581 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     8641 tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat tcgagctcgg
     8701 tacccgggga tcggcgcgcc ctatggacgt caacatggtg gagcacgaca ctctggtcta
     8761 ctccaaaaat gtcaaagata cagtctcaga agatcaaagg gctattgaga cttttcaaca
     8821 aaggataatt tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga
     8881 aaggacagta gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc
     8941 tatcattcaa gatctctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag
     9001 catcgtggaa aaagaagagg ttccaaccac gtctacaaag caagtggatt gatgtgacat
     9061 ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat
     9121 ataaggaagt tcatttcatt tggagaggac acgctcgagt ataagagctc atttttacaa
     9181 caattaccaa caacaacaaa caacaaacaa cattacaatt acatttacaa ttatcgatac
     9241 aatggtgcag gacacttcaa gtgctagcac aagtccaatt ttaaccagat ggtatataga
     9301 cactcgacct ctcacggcaa gtaccgccgc acttccgctg ctggaaacgc tgcaaccagc
     9361 tgatcagatc tccgtccaaa aatattatca tctcaaggac aagcacatgt cacttgcctc
     9421 caaccttttg aagtatttat tcgttcatcg aaattgccga ataccatgga gtagtattgt
     9481 catatcaaga acgcccgacc cgcacagaag gccttgctat atacctcctt caggttcaca
     9541 agaggactcc ttcaaagacg gatatacggg aatcaacgta gaattcaacg ttagtcacca
     9601 agccagcatg gtggcaatcg caggtacggc tttcacacct aatagcggtg gagatagcaa
     9661 actcaaaccg gaggtcggaa tagatataac atgtgtaaat gagaggcagg gcaggaatgg
     9721 agaagaaaga tccttggaat ccctgaggca gtatatagat atattcagcg aagtattcag
     9781 caccgcagag atggctaata taaggcgact tgatggggtt tcctcaagtt cccttagtgc
     9841 agacagactc gtagattatg gataccgatt attctacacc tattgggcct taaaagaggc
     9901 ttatatcaag atgacaggtg aggccttatt ggctccttgg ttgagagagc tggagttttc
     9961 caacgtggtc gctccagccg ctgtcgccga aagcggggac tctgctggag actttggcga
    10021 accgtacact ggcgtgcgta caacattgta taagaacctc gtagaggacg tacgaattga
    10081 ggtagctgct ttgggaggag attatctgtt cgcaacagcc gctagaggtg gtggtatagg
    10141 cgcatcctca cgtcctggtg gtggcccaga tggctctgga attagatccc aggacccttg
    10201 gaggccgttc aagaaactcg atatagagcg tgacatacag ccatgtgcta cgggcgtttg
    10261 taattgcctg tcctcaggat aggcttgatc ccccgtcgac agctagctat atcatcaatt
    10321 tatgtattac acataatatc gcactcagtc tttcatctac ggcaatgtac cagctgatat
    10381 aatcagttat tgaaatattt ctgaatttaa acttgcatca ataaatttat gtttttgctt
    10441 ggactataat acctgacttg ttattttatc aataaatatt taaactatat ttctttcaag
    10501 atcgctgtta gagctcggac ggcgcgccct atggactggc agacatactg tcccacaaat
    10561 gaagatggaa tctgtaaaag aaaacgcgtg aaataatgcg tctgacaaag gttaggtcgg
    10621 ctgcctttaa tcaataccaa agtggtccct accacgatgg aaaaactgtg cagtcggttt
    10681 ggctttttct gacgaacaaa taagattcgt ggccgacagg tgggggtcca ccatgtgaag
    10741 gcatcttcag actccaataa tggagcaatg acgtaagggc ttacgaaata agtaagggta
    10801 gtttgggaaa tgtccactca cccgtcagtc tataaatact tagcccctcc ctcattgtta
    10861 agggagcaaa atctcagaga gatagtccta gagagagaaa gagagcaagt agcctagaag
    10921 tagtcaaggc ggcgaagtat tcaggcacgt ggccaggaag aagaaaagcc aagacgacga
    10981 aaacaggtaa gagctaagct taatggcatc ctttgaaaat tcactcagtg tcttaatagt
    11041 tggggctggc ttaggtggtt tggccgctgc tatagcactg cgaaggcaag gacatgtagt
    11101 caagatatac gatagttctt cattcaaggc cgaattggga gcaggattag ctgtaccacc
    11161 caatactctc cgttccctgc aacaactggg ctgtaataca gaaaacctga acggagtaga
    11221 caacctctgc tttacggcca tgggatacga cgggagtgta ggtatgatga ataatatgac
    11281 cgactaccgt gaggcatacg gcacttcctg gataatggta catcgagtgg atctccacaa
    11341 cgagctgatg cgagtcgcct tggatccggg cgggttaggg cctcctgcca ctcttcacct
    11401 taaccatcgt gttacgttct gcgatgtgga tgcctgtaca gtcacgttta cgaacgggac
    11461 gacacagtct gctgacttga ttgtcggagc tgatggtatt aggtctacga ttcgaagatt
    11521 cgttctggag gaggacgtga ctgttccagc tagcggaatt gtcggctttc gatggttggt
    11581 ccaggccgat gctctcgacc catatcccga actggattgg attgtcaaaa aaccaccctt
    11641 gggagccagg ttgatttcta ctccacagaa cccgcagagt ggcgtaggac tggccgatag
    11701 acgaacgatc attatttacg catgcagagg tgggaccatg gttaatgtat tagccgtaca
    11761 cgacgacgaa agagatcaaa acactgctga ttggtccgtt cccgcatcaa aggatgattt
    11821 attcagagtg tttcacgatt accatccaag attccgacga ctcttagagt tagcacagga
    11881 cattaattta tggcagatga gggtggtccc ggtcttgaaa aaatgggtga acaagagggt
    11941 atgcttgctg ggtgatgccg cccatgcttc actgcccaca cttggacaag gcttcgggat
    12001 ggggttggag gatgcagtcg ctttgggcac gttgttgcct aagggaacga ccgcatcaca
    12061 gatcgagaca aggctggccg tttacgaaca gctgcgaaag gatcgagctg agtttgtcgc
    12121 cgcagagagc tatgaggagc aatatgtccc agagatgaga gggctgtacc ttaggtccaa
    12181 agaattgaga gacagagtga tgggttatga tattaaggta gagtcagaga aggttttgga
    12241 aacactgttg cgatcatcca actcagcttg agcttgtcct gctttaatga gatatgcgag
    12301 aagcctatga tcgcatgata tttgctttca attctgttgt gcacgttgta aaaaacctga
    12361 gcatgtgtag ctcagatcct taccgccggt ttcggttcat tctaatgaat atatcacccg
    12421 ttactatcgt atttttatga ataatattct ccgttcaatt tactgattgt accctactac
    12481 ttatatgtac aatattaaaa tgaaaacaat atattgtgct gaataggttt atagcgacat
    12541 ctatgataga gcgccacaat aacaaacaat tgcgttttat tattacaaat ccaattttaa
    12601 aaaaagcggc agaaccggtc aaacctaaaa gactgattac ataaatctta ttcaaatttc
    12661 aaaagtgccc caggggctag tatctacgac acaccgagcg gcgaactaat aacgctcact
    12721 gaagggaact ccggttcccc gccggcgcgc atgggtgaga ttccttgaag ttgagtattg
    12781 gccgtccgct ctaccgaaag ttacgggcac cattcaaccc ggtccagcac ggcggccggg
    12841 taaccgactt gctgccccga gaattatgca gcattttttt ggtgtatgtg ggccccaaat
    12901 gaagtgcagg tcaaaccttg acagtgacga caaatcgttg ggcgggtcca gggcgaattt
    12961 tgcgacaaca tgtcgaggct cagcaggacc gctgttagag ctcccggggc gcgccctatg
    13021 gagtggcaga catactgtcc cacaaatgaa gatggaatct gtaaaagaaa acgcgtgaaa
    13081 taatgcgtct gacaaaggtt aggtcggctg cctttaatca ataccaaagt ggtccctacc
    13141 acgatggaaa aactgtgcag tcggtttggc tttttctgac gaacaaataa gattcgtggc
    13201 cgacaggtgg gggtccacca tgtgaaggca tcttcagact ccaataatgg agcaatgacg
    13261 taagggctta cgaaataagt aagggtagtt tgggaaatgt ccactcaccc gtcagtctat
    13321 aaatacttag cccctccctc attgttaagg gagcaaaatc tcagagagat agtcctagag
    13381 agagaaagag agcaagtagc ctagaagtag tcaaggcggc gaagtattca ggcacgtggc
    13441 caggaagaag aaaagccaag acgacgaaaa caggtaagag ctaagcttaa tgaatagtag
    13501 taaaaaccct ccatccactc tgttagatgt ttttctggat accgctagga accttgatac
    13561 agcatccaga aatgtcctgg agtgcggaga acacaggtgg agttacagag agttggacac
    13621 agtgtcatcc gcattggccc aacaccttcg atacactgtg ggtcttagcc ccactgtagc
    13681 cgtgattagc gagaatcacc cctacatact tgcattaatg ctcgccgtat ggaaactcgg
    13741 cggaacgttc gcaccgatag atgttcattc cccagccgaa cttgttgctg gaatgttgaa
    13801 tattgtaagc ccgtcatgtc ttgtcattcc tagctctgat gtgacaaatc agacattggc
    13861 atgcgatctt aatataccgg ttgttgcatt ccacccacat caaagtacaa ttcccgaact
    13921 caacaagaag tatctcacag attctcagat cagtcctgac ctgcccttcc ccgaccctaa
    13981 tcgacctgct ttgtacttat tcacgtcatc tgcaacgtct aggtccaatt tgaagtgcgt
    14041 gccacttaca cacacattca tattgcgaaa ctccttaagt aaacgagcat ggtgcaaaag
    14101 aatgaggcca gagacagatt ttgatggtat tagggtcctg gggtgggccc cctggagcca
    14161 tgtcctcgct catatgcagg acattggccc tcttacatta ttgaatgccg ggtgctacgt
    14221 atttgccacg accccatcta cctacccgac ggagctgaag gatgaccgtg atgttattag
    14281 ttgcgccgca aatgccgtaa tgtacaaagg tgttaaatcc ttcgcatgcc tcccattcgt
    14341 gcttggggga ctcaaggctt tatgtgagtc agagcccagt gttaaggcac aactgcaagt
    14401 ggaggaacga gcccagcttt tgaagtcact ccaacacatg aaaatcctgg aatgcggggg
    14461 ggcaatgctc gaagtgagtg tggcaagctg ggctatagag aataggattc ctatatctat
    14521 tgggattggt atgacagaga caggcggcgc tcttttcgct gggccggtcc aagcaattca
    14581 aaccggcttt agcagtgaag ataaatttat cgaggacgca acctacttgc tggtcaagga
    14641 cgattacgag agccacgcag aggaggacat aaacgagggc gagttagtgg taaagtccag
    14701 aatgttacct aggggttatt tgggctataa cgacccctct ttctctgttg acgatgctgg
    14761 ttgggttact ttcaaaactg gagatcgata ttcagtcact cctgacggca aattctcctg
    14821 gcttggtcga aatactgatt tcattcagat gacgagtggt gagactctcg atccccgtcc
    14881 gattgagtcc ctcctttgtg agtccagcct tatatcacga gcctgtgtca tcggggataa
    14941 gttcctgaac ggaccagcca cagctgtatg cgctattata gaactcgaac caacgactgt
    15001 tgagaaaggc caggctcaca gcagggatat tgcccgaatc tttgccccca tcaatcgaga
    15061 tcttcctccc ccgttacgta tagcatggag tcacgtactt gtacttcagc caagcgagaa
    15121 aataccgatg acgaaaaagg gtacaatctt ccgtaagaaa atcgaacagg ttttcggttc
    15181 cgctttagga ggcagtagcg gcgacaactc acaagcaacc actgacgctt cagtagttag
    15241 gagggatgag ttgagcaaca cggtcaaaca tatcataagc agagttttgg gagtctccga
    15301 cgatgaactc ctttggacac tgagctttgc tgagcttggc atgacgtccg ctcttgctac
    15361 tagaatagca aatgaattga acgaagttct ggtaggtgtg aacctcccca tcaatgcatg
    15421 ctacattcat gtggacctgc cttctttgtc aaatgccgtg tatgcaaagt tagcacacct
    15481 taaactcccg gataggacgc ccgagccaag gaaggcccca gttgaaaatc ccggtggaaa
    15541 ggaaatcgta atagtaggtc aggcttttag gcttccgggg agtataaatg atgtcgcttc
    15601 tttgagggac gcttttttgg ctagacaagc atccagtatt attactgaaa taccgccaga
    15661 tagatgggac cacgcatcat tttatccaaa agacatacga tttaataaag cagggctcgt
    15721 cgacatcgct aactacgacc acagtttttt cggattgaca gccacggagg cactgtacct
    15781 gtcaccaacg atgcgtttgg cacttgaagt gtcctttgaa gccttggaga atgctaatat
    15841 acccgtgtcc cagttaaagg gctctcaaac agctgtatat gtcgcaacca ccgacgacgg
    15901 gtttgagaca ctgctgaatg ctgaggcagg atatgatgca tacacacgat tctatggcac
    15961 tggtagggcc gcctctacgg ccagtggacg aattagttat ttactcgacg tacacggccc
    16021 ttcaattacc gtagacactg cttgttcagg cggtgctgtt tgcatagacc aggccataga
    16081 ctacctccaa tcctctagcg cagctgatac cgccataatc tgcgccagca acactcactg
    16141 ctggccaggt tccttcatgt ttctgagtgc tcaaggtatg gtaagttccg gggggcgatg
    16201 tgcaactttt acgacggatg ccgacggcta tgtaccctct gaaggtgctg tggcatttat
    16261 cctcaaaacc cgagaagcag ctatgagaga taaagatact atcttggcaa ctatcaaagc
    16321 aacgcagatt agtcataatg gtcgttccca aggtttagtc gcaccaaacg tcaattcaca
    16381 agctgattta cacagaagct tattgcagaa agctggtttg agtccagccg acattcactt
    16441 catcgaagct cacgggacag gaacgtcact cggtgacctt agtgaaatac aagcaattaa
    16501 tgatgcctac acgtcatctc agcctcgaac agccggaccc ttaatagtaa gcgcaagtaa
    16561 aaccgtcatt ggtcacacag agcctgctgg accgttggta ggcatgctct cagttctcaa
    16621 ctcctttaaa gagggcgcag ttcccggctt agctcacctg acagcagata acctcaatcc
    16681 ggctttagac tgctcctccg tccctttact tatcccctac cagcctgtcc acttggcagc
    16741 tcctaagcca cacagggctg ctgtgatgag ttatggcttc tccggcactc ttggaggtat
    16801 agttcttgaa gcaccggatg aggaaaggct tgaggaagaa ccacccaacg ataagcctat
    16861 gttatttgtt gttagcgcca aaactcatac tgcattgatt gagtaccttg gacgatacct
    16921 tgaatttctg ctccaggcca atccccaaga cttctgcgat atctgttaca ctagctgcgt
    16981 tggacgtgag cattacaggt accgttttgc atgtgtggca aacgatatgg aagatttaat
    17041 tggccaactt cagaagaggc ttgggtctaa ggttccacct aaaccgtcct ataaaagagg
    17101 agccctcgct tttgctttca gtggacaagg gacccagttt cgtggaatgg caacagaatt
    17161 ggctaaggca tatagtggtt ttcgtaagat agtcagtgat cttgctaaaa gagcatcaga
    17221 actgtccggc catgcaatcg acaggtttct gcttgcatat gacattgggg ctgagaatgt
    17281 tgcaccggat agtgaagctg atcaaatctg catattcgtt taccaatgta gtgttctcag
    17341 atggctccaa acaatgggga ttcgtccgtc agccgtgatc ggtcacagcc tcggtgagat
    17401 tagcgcatcc gtagctgctg gagcattgtc cttggactct gcccttgacc tcgtaataag
    17461 tagagccagg ttactgaggt catctacgaa tgccccagct ggcatggcag ctatgtctgc
    17521 atcccaggat gaagtagtcg aactcatagg aaagctcgat ttagataaag ctaatagcct
    17581 cagcgtgtcc gtaataaacg gcccccagaa caccgttgtt tcaggatctt ctgcagcaat
    17641 agaaagtatc gttgctctcg caaagggacg aaaaatcaag gccagcgcac tcaacattaa
    17701 ccaggcattc catagtcctt acgtcgattc tgcagtaccc ggcttacgag cttggagtga
    17761 gaaacatata tcatcagctc gaccattgca aatccctttg tactctaccc tcttgggcgc
    17821 tcaagtttct gagggtcaaa tgttaaatcc cgatcactgg gtagaccatg ctaggaaacc
    17881 cgttcagttc gcacaagcag ccactataat gaaggaatct ttcaccggag taataatcga
    17941 tattggacct caagttgtag catggtccct gctcttaagc aatggtctta cttccgtgac
    18001 cgcactcgcc gcaaagcgtg gccgttcaca gcaagtggcc ttcctctccg ccttagctga
    18061 tctttatcag gactacggcg tcgtaccaga ctttgttgga ctgtacgcac aacaggagga
    18121 cgcttccaga ctgaaaaaga ccgatatctt aacttacccc tttcagaggg ttcgacgtta
    18181 cccttccttt ataccctcta gaagggcacc cacccacgct catgtccaag acgaggaaac
    18241 tctgtcatca ggatcatcaa ctccaacctt agaaaatact gacctggaca gtgggaagga
    18301 atctctgatg ggaccaacaa gagggctttt gcgagtcgat gatcttaggg actcaatagt
    18361 cagttcagtc aaagatgtcc tggaattgaa gagtaacgaa gatctggacc tcagcgagtc
    18421 ccttaatgct cttggcatgg actctataat gttcgctcaa ttacgaaaaa ggatagggga
    18481 gggcctcggc ctgtccgttc caatggtttt tctcagcgat gccttcagta ttggagaaat
    18541 ggtcagtaac ctcgtggaac aggccgaagc ttccgaggac aattgagctt atatgaagat
    18601 gaagatgaaa tatttggtgt gtcaaataaa aagcttgtgt gcttaagttt gtgttttttt
    18661 cttggcttgt tgtgttatga atttgtggct ttttctaata ttaaatgaat gtaagatcac
    18721 attataatga ataaacaaat gtttctataa tccattgtga atgttttgtt ggatctcttc
    18781 tgcagcatat aactactgta tgtgctatgg tatggactat ggaatatgat taaagataag
    18841 cgctgttaga gctctcccgg cgcgccctat ggacagattt gccttttcaa tttcagaaag
    18901 aatgctaacc cacagatggt tagagaggct tacgcagcag gtatcatcaa gacgatctac
    18961 ccgagcaata atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa
    19021 agattcagga ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt
    19081 actattccag tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt
    19141 ggagtctcta aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata
    19201 gaggacctaa cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga
    19261 ctcaatgaca agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc
    19321 aaaaatatca aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg
    19381 gtaatatccg gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag
    19441 atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc
    19501 gttgaagatg cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc
    19561 gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc
    19621 actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa
    19681 ggaagttcat ttcatttgga gagaacacgg gggactaatg cgtataaata tctctctgtc
    19741 tagcttattc gagagattaa gcaaactcag ctctcgtagc atagctatta catgtggtgt
    19801 cgtcctcgcc tctgctattg cttttcccat catacgacga gattatcaga cgttcttaga
    19861 ggttggtcct agctatgccc ctcaaaattt tcgaggctac attatcgttt gcgtactcag
    19921 cttgttccga caagagcaaa aagggttggc aatctacgac agactcccag agaagagaag
    19981 atggctggcc gatctcccat ttcgtgaagg tactagacct agtataacat cacacataat
    20041 acagagacag cgaactcaac ttgtagacca agagtttgcc acacgagaac tgatagacaa
    20101 agttattccc agggtgcaag cacgacatac agataaaaca tttttgagca cgagcaagtt
    20161 tgaatttcat gctaaagcca ttttcctgtt gccaagtatt cctataaacg accccttaaa
    20221 catacctagt catgatacgg ttcgtagaac gaaacgagag attgcacaca tgcatgacta
    20281 ccacgactgt actcttcact tagctcttgc agctcaagat ggaaaagaag tcctgaaaaa
    20341 aggctggggc caacgtcatc cgcttgccgg gccgggtgta cctgggccgc cgacagaatg
    20401 gactttctta tacgcacccc gtaatgagga agaagctagg gtagtagaaa tgattgtcga
    20461 ggcaagtatc ggatacatga ctaatgatcc ggcaggtaaa atcgtcgaga atgccaaatg
    20521 agcttgtcct gctttaatga gatatgcgag aagcctatga tcgcatgata tttgctttca
    20581 attctgttgt gcacgttgta aaaaacctga gcatgtgtag ctcagatcct taccgccggt
    20641 ttcggttcat tctaatgaat atatcacccg ttactatcgt atttttatga ataatattct
    20701 ccgttcaatt tactgattgt accctactac ttatatgtac aatattaaaa tgaaaacaat
    20761 atattgtgct gaataggttt atagcgacat ctatgataga gcgccacaat aacaaacaat
    20821 tgcgttttat tattacaaat ccaattttaa aaaaagcggc agaaccggtc aaacctaaaa
    20881 gactgattac ataaatctta ttcaaatttc aaaagtgccc caggggctag tatctacgac
    20941 acaccgagcg gcgaactaat aacgctcact gaagggaact ccggttcccc gccggcgcgc
    21001 atgggtgaga ttccttgaag ttgagtattg gccgtccgct ctaccgaaag ttacgggcac
    21061 cattcaaccc ggtccagcac ggcggccggg taaccgactt gctgccccga gaattatgca
    21121 gcattttttt ggtgtatgtg ggccccaaat gaagtgcagg tcaaaccttg acagtgacga
    21181 caaatcgttg ggcgggtcca gggcgaattt tgcgacaaca tgtcgaggct cagcaggacc
    21241 gctgttagag ctcagtg
//
