LOCUS       sequence-296193-        6944 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses N-terminus to aa396 of Flp recombinase fused to nMag CID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6944)
  AUTHORS   Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, 
            Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J,
            Wong WW
  TITLE     High-performance chemical- and light-inducible recombinases in 
            mammalian cells and mice.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7.
  PUBMED    31649244
REFERENCE   2  (bases 1 to 6944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4845. doi"; pages: " 
            10.1038/s41467-019-12800-7"
COMMENT     Sequence Label: pBW2677_pCAG-PV1-FlpO-N396-L1-nMag-NLS-BGHpA
FEATURES             Location/Qualifiers
     source          1..6944
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..657
                     /label=chicken beta-actin promoter
     intron          658..1674
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1598..1620
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1682..1701
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3489..3509
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3519..3743
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(3983..4002)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4048..4103
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4102..4121)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4464..4480)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4464..4480)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4477..4499)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4488..4504
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4512..4518,4519..4536,4537..4542))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4557..4578
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      4663..4797
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4817..4951
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4854..4873)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4908..4927
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5019..5036)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5190..5778)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5270..5289)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5949..6740,6741..6809))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6572..6591
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6810..6914)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg gggcgcgcca ggcggggcgg ggcggggcga ggggcggggc
      541 ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct
      601 tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga
      661 gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc
      721 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg
      781 ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc
      841 cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg
      901 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
      961 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
     1081 gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc
     1321 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
     1381 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
     1441 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     1501 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
     1561 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     1621 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     1681 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattgat taattcgagc
     1741 gaaggcgcgc ccattactcg catccattct caggctgtct cgtctcgtct cgcacgcgtg
     1801 ccgccaccat gagccagttc gacatcctgt gcaagacccc ccccaaggtg ctggtgcggc
     1861 agttcgtgga gagattcgag aggcccagcg gcgagaagat cgccagctgt gccgccgagc
     1921 tgacctacct gtgctggatg atcacccaca acggcaccgc catcaagagg gccaccttca
     1981 tgagctacaa caccatcatc agcaacagcc tgagcttcga catcgtgaac aagagcctgc
     2041 agttcaagta caagacccag aaggccacca tcctggaggc cagcctgaag aagctgatcc
     2101 ccgcctggga gttcaccatc atcccttaca acggccagaa gcaccagagc gacatcaccg
     2161 acatcgtgtc cagcctgcag ctgcagttcg agagcagcga ggaggccgac aagggcaaca
     2221 gccacagcaa gaagatgctg aaggccctgc tgtccgaggg cgagagcatc tgggagatca
     2281 ccgagaagat cctgaacagc ttcgagtaca ccagcaggtt caccaagacc aagaccctgt
     2341 accagttcct gttcctggcc acattcatca actgcggcag gttcagcgac atcaagaacg
     2401 tggaccccaa gagcttcaag ctggtgcaga acaagtacct gggcgtgatc attcagtgcc
     2461 tggtgaccga gaccaagaca agcgtgtcca ggcacatcta ctttttcagc gccagaggca
     2521 ggatcgaccc cctggtgtac ctggacgagt tcctgaggaa cagcgagccc gtgctgaaga
     2581 gagtgaacag gaccggcaac agcagcagca acaagcagga gtaccagctg ctgaaggaca
     2641 acctggtgcg cagctacaac aaggccctga agaagaacgc cccctacccc atcttcgcta
     2701 tcaagaacgg ccctaagagc cacatcggca ggcacctgat gaccagcttt ctgagcatga
     2761 agggcctgac cgagctgaca aacgtggtgg gcaactggag cgacaagagg gcctccgccg
     2821 tggccaggac cacctacacc caccagatca ccgccatccc cgaccactac ttcgccctgg
     2881 tgtccaggta ctacgcctac gaccccatca gcaaggagat gatcgccctg aaggacgaga
     2941 ccaaccccat cgaggagtgg cagcacatcg agcagctgaa gggcagcgcc gagggctccg
     3001 gagggtctgg ctccggatca agtggtggca gcggtaccca tactctttat gcccccggtg
     3061 gatatgacat tatgggatat ctggaccaga tcggcaaccg gccaaacccg caggtggaac
     3121 tgggccccgt ggatacatcc tgcgccttga ttctttgtga cctgaaacag aaagacaccc
     3181 cgatagttta cgcgagtgaa gccttcctct acatgacagg ttacagcaac gcagaggtgc
     3241 tgggccggaa ttgccggttt ctgcaaagcc ctgacggcat ggtgaagccc aagagcaccc
     3301 ggaagtacgt ggatagtaac acaatcaata ctatgcgcaa ggcaatcgac aggaatgccg
     3361 aggtgcaggt tgaagtagtc aattttaaaa agaatggaca gcgatttgtt aatttcctga
     3421 ctatgatacc tgttagggac gaaacaggcg agtatcgata ctctatggga ttccagtgcg
     3481 aaacagaacc caagaaaaag cggaaggtgt gagaattcct gtgccttcta gttgccagcc
     3541 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     3601 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     3661 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     3721 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     3781 gtatccccac gcgccctgta gcgtgtaccg ctgggagttc gtagacggaa acaaacgcag
     3841 aatccaagcg cggccgccca ggatacatag attaccacaa ctccgagccc ttccaccgct
     3901 agcgcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc
     3961 tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga
     4021 agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt
     4081 gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac
     4141 atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga
     4201 acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct
     4261 tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat
     4321 ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc
     4381 tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc
     4441 agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     4501 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     4561 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     4621 tcgtgccagc ggatccgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     4681 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     4741 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     4801 gcttttgcaa aaagctaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     4861 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     4921 actcatcaat gtatcttatc atgtctggat ccgctgcatt aatgaatcgg ccaacgcgcg
     4981 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     5041 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     5101 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     5161 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     5221 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     5281 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     5341 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     5401 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     5461 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     5521 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     5581 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     5641 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     5701 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     5761 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     5821 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     5881 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     5941 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     6001 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     6061 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     6121 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     6181 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     6241 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     6301 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     6361 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     6421 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     6481 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     6541 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     6601 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     6661 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     6721 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     6781 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     6841 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     6901 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ggtc
//
