LOCUS       sequence-296195-        9170 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The pZJQIBEBT003 plasmid contains the coding sequence of 
            eMutS-L157C-G233C gene which has the ability of error removal in the
            gene synthesis process..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9170)
  AUTHORS   Zhang J, Wang Y, Chai B, Wang J, Li L, Liu M, Zhao G, Yao L, Gao X, 
            Yin Y, Xu J
  TITLE     Efficient and Low-Cost Error Removal in DNA Synthesis by a 
            High-Durability MutS.
  JOURNAL   ACS Synth Biol. 2020 Apr 17;9(4):940-952. doi: 
            10.1021/acssynbio.0c00079. Epub 2020 Mar 13.
  PUBMED    32135061
REFERENCE   2  (bases 1 to 9170)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.0c00079"; journalName: "ACS Synth Biol"; date: 
            "2020-04-17- 17"; volume: "9"; issue: "4"; pages: "940-952"
COMMENT     Sequence Label: pZJQIBEBT003
FEATURES             Location/Qualifiers
     source          1..9170
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    20..44
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             59..81
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             665..676
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             join(3170..3172,3173..3175,3176..3886)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3215..3236)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3476..3495)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3823..3844
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3902..3919
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3972..3990)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      3986..4033
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      4070..4525
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4157..4176)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4367..4388
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4551..4655
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4656..4724,4725..5516)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4874..4893)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5687..6275
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6176..6195
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6429..6446
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6461..6600
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6526..6545)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6686..6708
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6702..6893)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(7702..8784)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     8746..8765
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(8785..8862)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     9068..9087
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
       61 tgtttaactt taagaaggag atatacatat ggctagcatg agtgcaatag aaaatttcga
      121 cgcccatacg cccatgatgc agcagtatct caggctgaaa gcccagcatc ccgagatcct
      181 gctgttttac cggatgggtg atttttatga actgttttat gacgacgcaa aacgcgcgtc
      241 gcaactgctg gatatttcac tgaccaaacg cggtgcttcg gcgggagagc cgatcccgat
      301 ggcggggatt ccctaccatg cggtggaaaa ctatctcgcc aaactggtga atcagggaga
      361 gtccgttgcc atctgcgaac aaattggcga tccggcgacc agcaaaggtc cggttgagcg
      421 caaagttgtg cgtatcgtta cgccaggcac catcagcgat gaagccctgt tgcaggagcg
      481 tcaggacaac ctgctggcgg ctatctggca ggacagcaaa ggtttcggct acgcgacgct
      541 ggatatcagt tccgggcgtt ttcgctgcag cgaaccggct gaccgcgaaa cgatggcggc
      601 agaactgcaa cgcactaatc ctgcggaact gctgtatgca gaagattttg ctgaaatgtc
      661 gttaattgaa ggccgtcgcg gcctgcgccg tcgcccgctg tgggagtttg aaatcgacac
      721 cgcgcgccag cagttgaatc tgcaatttgg gacccgcgat ctggtcggtt ttggcgtcga
      781 gaacgcgccg cgctgccttt gtgctgccgg ttgtctgttg cagtatgcga aagataccca
      841 acgtacgact ctgccgcata ttcgttccat caccatggaa cgtgagcagg acagcatcat
      901 tatggatgcc gcgacgcgtc gtaatctgga aatcacccag aacctggcgg gtggtgcgga
      961 aaatacgctg gcttctgtgc tcgactgcac cgtcacgccg atgggcagcc gtatgctgaa
     1021 acgctggctg catatgccag tgcgcgatac ccgcgtgttg cttgagcgcc agcaaactat
     1081 tggcgcattg caggatttca ccgccgggct acagccggta ctgcgtcagg tcggcgacct
     1141 ggaacgtatt ctggcacgtc tggctttacg aactgctcgc ccacgcgatc tggcccgtat
     1201 gcgccacgct ttccagcaac tgccggagct gcgtgcgcag ttagaaactg tcgatagtgc
     1261 accggtacag gcgctacgtg agaagatggg cgagtttgcc gagctgcgcg atctgctgga
     1321 gcgagcaatc atcgacacac cgccggtgct ggtacgcgac ggtggtgtta tcgcatcggg
     1381 ctataacgaa gagctggatg agtggcgcgc gctggctgac ggcgcgaccg attatctgga
     1441 gcgtctggaa gtccgcgagc gtgaacgtac cggcctggac acgctgaaag ttggctttaa
     1501 tgcggtgcac ggctactaca ttcaaatcag ccgtgggcaa agccatctgg cacccatcaa
     1561 ctacatgcgt cgccagacgc tgaaaaacgc cgagcgctac atcattccag agctaaaaga
     1621 gtacgaagat aaagttctca cctcaaaagg caaagcactg gcactggaaa aacagcttta
     1681 tgaagagctg ttcgacctgc tgttgccgca tctggaagcg ttgcaacaga gcgcgagcgc
     1741 gctggcggaa ctcgacgtgc tggttaacct ggcggaacgg gcctataccc tgaactacac
     1801 ctgcccgacc ttcattgata aaccgggcat tcgcattacc gaaggtcgcc atccggtagt
     1861 tgaacaagta ctgaatgagc catttatcgc caacccgctg aatctgtcgc cgcagcgccg
     1921 catgttgatc atcaccggtc cgaacatggg cggtaaaagt acctatatgc gccagaccgc
     1981 actgattgcg ctgatggcct acatcggcag ctatgtaccg gcacaaaaag tcgagattgg
     2041 acctatcgat cgcatcttta cccgcgtagg cgcggcagat gacctggcgt ccgggcgctc
     2101 aacctttatg gtggagatga ctgaaaccgc caatatttta cataacgcca ccgaatacag
     2161 tctggtgtta atggatgaga tcgggcgtgg aacgtccacc tacgatggtc tgtcgctggc
     2221 gtgggcgtgc gcggaaaatc tggcgaataa gattaaggca ttgacgttat ttgctaccca
     2281 ctatttcgag ctgacccagt taccggagaa aatggaaggc gtcgctaacg tgcatctcga
     2341 tgcactggag cacggcgaca ccattgcctt tatgcacagc gtgcaggatg gcgcggcgag
     2401 caaaagctac ggcctggcgg ttgcagctct ggcaggcgtg ccaaaagagg ttattaagcg
     2461 cgcacggcaa aagctgcgtg agctggaaag catttcgccg aacgccgccg ctacgcaagt
     2521 ggatggtacg caaatgtctt tgctgtcagt accagaagaa acttcgcctg cggtcgaagc
     2581 tctggaaaat cttgatccgg attcactcac cccgcgtcag gcgctggagt ggatttatcg
     2641 cttgaagagc ctggtggagc tcatgccggt atcaggcaat ttgaaggttg aattctacaa
     2701 cagcaatcct tcagatacta ctaactcaat caatcctcag ttcaaggtta ctaataccgg
     2761 aagcagtgca attgatttgt ccaaactcac attgagatat tattatacag tagacggaca
     2821 gaaagatcag accttctggt gtgaccatgc tgcaataatc ggcagtaacg gcagctacaa
     2881 cggaattact tcaaatgtaa aaggaacatt tgtaaaaatg agttcctcaa caaataacgc
     2941 agacacctac cttgaaataa gctttacagg cggaactctt gaaccgggtg cacatgttca
     3001 gatacaaggt agatttgcaa agaatgactg gagtaactat acacagtcaa atgactactc
     3061 attcaagtct gcttcacagt ttgttgaatg ggatcaggta acagcatact tgaacggtgt
     3121 tcttgtatgg ggtaaagaac ccaggcctgg cggcctcgag ggctcttcca tggtgagcaa
     3181 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     3241 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     3301 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     3361 cttgacctac ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt
     3421 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     3481 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     3541 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     3601 caactacaac agccacaagg tctatatcac cgccgacaag cagaagaacg gcatcaaggt
     3661 gaacttcaag acccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     3721 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     3781 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     3841 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaaggcgg ccgcactcga
     3901 gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt
     3961 ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt
     4021 gaggggtttt ttgctgaaag gaggaactat atccggattg gcgaatggga cgcgccctgt
     4081 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     4141 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     4201 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     4261 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     4321 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     4381 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     4441 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     4501 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     4561 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     4621 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     4681 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     4741 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     4801 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     4861 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     4921 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     4981 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     5041 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     5101 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     5161 aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc
     5221 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     5281 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     5341 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     5401 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     5461 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     5521 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     5581 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     5641 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     5701 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     5761 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     5821 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     5881 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     5941 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     6001 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     6061 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     6121 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     6181 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     6241 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     6301 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     6361 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     6421 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc
     6481 ttacgcatct gtgcggtatt tcacaccgca atggtgcact ctcagtacaa tctgctctga
     6541 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg
     6601 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     6661 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     6721 tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca
     6781 cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc
     6841 tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc
     6901 tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg
     6961 ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa
     7021 caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc
     7081 ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc
     7141 cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa
     7201 ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac
     7261 gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc
     7321 cgggtcctca acgacaggag cacgatcatg cgcacccgtg gggccgccat gccggcgata
     7381 atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg
     7441 gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag
     7501 cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata
     7561 aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg
     7621 actgggttga aggctctcaa gggcatcggt cgagatcccg gtgcctaatg agtgagctaa
     7681 cttacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     7741 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt
     7801 ggtttttctt ttcaccagtg agacgggcaa cagctgattg cccttcaccg cctggccctg
     7861 agagagttgc agcaagcggt ccacgctggt ttgccccagc aggcgaaaat cctgtttgat
     7921 ggtggttaac ggcgggatat aacatgagct gtcttcggta tcgtcgtatc ccactaccga
     7981 gatatccgca ccaacgcgca gcccggactc ggtaatggcg cgcattgcgc ccagcgccat
     8041 ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc tcattcagca tttgcatggt
     8101 ttgttgaaaa ccggacatgg cactccagtc gccttcccgt tccgctatcg gctgaatttg
     8161 attgcgagtg agatatttat gccagccagc cagacgcaga cgcgccgaga cagaacttaa
     8221 tgggcccgct aacagcgcga tttgctggtg acccaatgcg accagatgct ccacgcccag
     8281 tcgcgtaccg tcttcatggg agaaaataat actgttgatg ggtgtctggt cagagacatc
     8341 aagaaataac gccggaacat tagtgcaggc agcttccaca gcaatggcat cctggtcatc
     8401 cagcggatag ttaatgatca gcccactgac gcgttgcgcg agaagattgt gcaccgccgc
     8461 tttacaggct tcgacgccgc ttcgttctac catcgacacc accacgctgg cacccagttg
     8521 atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga
     8581 ggtggcaacg ccaatcagca acgactgttt gcccgccagt tgttgtgcca cgcggttggg
     8641 aatgtaattc agctccgcca tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg
     8701 gctggcctgg ttcaccacgc gggaaacggt ctgataagag acaccggcat actctgcgac
     8761 atcgtataac gttactggtt tcacattcac caccctgaat tgactctctt ccgggcgcta
     8821 tcatgccata ccgcgaaagg ttttgcgcca ttcgatggtg tccgggatct cgacgctctc
     8881 ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg
     8941 ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc
     9001 ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt
     9061 ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc
     9121 cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
//
