LOCUS       sequence-296233-        1059 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express APEX2-Galectin-9 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Jia J, Abudu YP, Claude-Taupin A, Gu Y, Kumar S, Choi SW, Peters R, 
            Mudd MH, Allers L, Salemi M, Phinney B, Johansen T, Deretic V
  TITLE     Galectins Control mTOR in Response to Endomembrane Damage.
  JOURNAL   Mol Cell. 2018 Apr 5;70(1):120-135.e8. doi: 
            10.1016/j.molcel.2018.03.009.
  PUBMED    29625033
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2018.03"; journalName: "Mol Cell"; date: 
            "2018-04-5- 5"; volume: "70"; issue: "1"; pages: "120-135.e8"
COMMENT     Sequence Label: pJJiaDEST-APEX2-GAL9
FEATURES             Location/Qualifiers
     source          1..1059
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     gap             4..28
                     /estimated_length=25
     primer_bind     77..96
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        77..95
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      115..124
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             124..147
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             148..894
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
ORIGIN
        1 abcnnnnnnn nnnnnnnnnn nnnnnnnnga gctctctggn nnntagagaa cccactgctt
       61 actggcttat cgaaattaat acgactcact atagggagac ccaagcttgc ggccgccacc
      121 atggactaca aggatgacga cgataaggga aagtcttacc caactgtgag tgctgattac
      181 caggacgccg ttgagaaggc gaagaagaag ctcagaggct tcatcgctga gaagagatgc
      241 gctcctctaa tgctccgttt ggcattccac tctgctggaa cctttgacaa gggcacgaag
      301 accggtggac ccttcggaac catcaagcac cctgccgaac tggctcacag cgctaacaac
      361 ggtcttgaca tcgctgttag gcttttggag ccactcaagg cggagttccc tattttgagc
      421 tacgccgatt tctaccagtt ggctggcgtt gttgccgttg aggtcacggg tggacctaag
      481 gttccattcc accctggaag agaggacaag cctgagccac caccagaggg tcgcttgccc
      541 gatcccacta agggttctga ccatttgaga gatgtgtttg gcaaagctat ggggcttact
      601 gaccaagata tcgttgctct atctgggggt cacactattg gagctgcaca caaggagcgt
      661 tctggatttg agggtccctg gacctctaat cctcttattt tcgacaactc atacttcacg
      721 gagttgttga gtggtgagaa ggaaggtctc cttcagctac cttctgacaa ggctcttttg
      781 tctgaccctg tattccgccc tctcgttgac aaatatgcag cggacgaaga tgccttcttt
      841 gctgattacg ctgaggctca ccaaaagctt tccgagcttg ggtttgctga tgccctgcag
      901 ctgcctcccc tggnncgcct gaccctggac ctcgaatcaa caagtttgta caaaaaagcn
      961 nnttcgcctt cagcggttcc cangctcccn atctgagccc agccgtcccc ttttctgggn
     1021 ctatccangg ggtctccagg acgnattnnn atcantgtc
//
