LOCUS       sequence-296235-        1062 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express APEX2-Galectin-8 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Jia J, Abudu YP, Claude-Taupin A, Gu Y, Kumar S, Choi SW, Peters R, 
            Mudd MH, Allers L, Salemi M, Phinney B, Johansen T, Deretic V
  TITLE     Galectins Control mTOR in Response to Endomembrane Damage.
  JOURNAL   Mol Cell. 2018 Apr 5;70(1):120-135.e8. doi: 
            10.1016/j.molcel.2018.03.009.
  PUBMED    29625033
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2018.03"; journalName: "Mol Cell"; date: 
            "2018-04-5- 5"; volume: "70"; issue: "1"; pages: "120-135.e8"
COMMENT     Sequence Label: pJJiaDEST-APEX2-GAL8
FEATURES             Location/Qualifiers
     source          1..1062
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..772
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAXAHQKLSELGFADA"
     CDS             779..805
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     protein_bind    818..842
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggnntacaag gnngacgacg ataagggaaa gtcttaccca actgtgagtg ctgattacca
       61 ggacgccgtt gagaaggcga agaagaagct cagaggcttc atcgctgaga agagatgcgc
      121 tcctctaatg ctccgtttgg cattccactc tgctggaacc tttgacaagg gcacgaagac
      181 cggtggaccc ttcggaacca tcaagcaccc tgccgaactg gctcacagcg ctaacaacgg
      241 tcttgacatc gctgttaggc ttttggagcc actcaaggcg gagttcccta ttttgagcta
      301 cgccgatttc taccagttgg ctggcgttgt tgccgttgag gtcacgggtg gacctaaggt
      361 tccattccac cctggaagag aggacaagcc tgagccacca ccagagggtc gcttgcccga
      421 tcccactaag ggttctgacc atttgagaga tgtgtttggc aaagctatgg ggcttactga
      481 ccaagatatc gttgctctat ctgggggtca cactattgga gctgcacaca aggagcgttc
      541 tggatttgag ggtccctgga cctctaatcc tcttattttc gacaactcat acttcacgga
      601 gttgttgagt ggtgagaagg aaggtctcct tcagctacct tctgacaagg ctcttttgtc
      661 tgaccctgta ttccgccctc tcgttgacaa atatgcagcg gacgaagatg ccttctttgc
      721 tgattacgct gangctcacc aaaagctttc cgagcttggg tttgctgatg ccctgcagct
      781 gcctcccctg gagcgcctga ccctgnncct cgaatcaaca agtttgtaca aaaaagcagg
      841 cttcttgtcc ttaaacaacc tacagaatat catctataac ccggtaatcc cgtttgttgg
      901 caccattcct gatcagctgg ntcctnnnct ttgattgtga tacgtgggca tgttcctant
      961 gacgcagana gattccagnt ggntctgcan nannnngcag catgaaacct cganccgatg
     1021 tnnnttcatt tcantnnnnt ttcaannnnn nngcatnntt gc
//
