LOCUS       sequence-296237-        1074 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express APEX2-Galectin-3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Jia J, Abudu YP, Claude-Taupin A, Gu Y, Kumar S, Choi SW, Peters R, 
            Mudd MH, Allers L, Salemi M, Phinney B, Johansen T, Deretic V
  TITLE     Galectins Control mTOR in Response to Endomembrane Damage.
  JOURNAL   Mol Cell. 2018 Apr 5;70(1):120-135.e8. doi: 
            10.1016/j.molcel.2018.03.009.
  PUBMED    29625033
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2018.03"; journalName: "Mol Cell"; date: 
            "2018-04-5- 5"; volume: "70"; issue: "1"; pages: "120-135.e8"
COMMENT     Sequence Label: pJJiaDEST-APEX2-GAL3
FEATURES             Location/Qualifiers
     source          1..1074
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     57..76
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        57..75
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      95..104
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             104..127
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             882..908
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLXL"
ORIGIN
        1 ancananctc tctggctaac tnnnnaggaa cccactgctt actggcttat cgaaattaat
       61 acgactcact atagggagac ccaagcttgc ggccgccacc atggactaca aggatgacga
      121 cgataaggga aagtcttacc caactgtgag tgctgattac caggacgccg ttgagaaggc
      181 gaagaagaag ctcagaggct tcatcgctga gaagagatgc gctcctctaa tgctccgttt
      241 ggcattccac tctgctggaa cctttgacaa gggcacgaag accggtggac ccttcggaac
      301 catcaagcac cctgccgaac tggctcacag cgctaacaac ggtcttgaca tcgctgttag
      361 gcttttggag ccactcaagg cggagttccc tattttgagc tacgccgatt tctaccagtt
      421 ggctggcgtt gttgccgttg aggtcacggg tggacctaag gttccattcc accctggaag
      481 agaggacaag cctgagccac caccagaggg tcgcttgccc gatcccacta agggttctga
      541 ccatttgaga gatgtgtttg gcaaagctat ggggcttact gaccaagata tcgttgctct
      601 atctgggggt cacactattg gagctgcaca caaggagcgt tctggatttg agggtccctg
      661 gacctctaat cctcttattt tcgacnactc atacttcacg gagttgttga gtggtgagaa
      721 ggaaggtctc cttcagctac cttctgacaa ggctcttttt gtctgaccct gtattccgcc
      781 ctctcgttga caaatatgca gcggacgaag atgccttctt tgctgattac gctgaggctc
      841 accaaaagct ttccgagctn gggnttgctg atgccctgca gctgcctccc ctggagcgcc
      901 tgancctggn cctcgaatca acaagtttgt acaaaaagcn nntccgcggc cgcccccttc
      961 ancatgnnga nanttttcgc tccatgatgc gttatctggt cnggaaaccc aaaccctcan
     1021 gnngnnctnn ncatggggna annagncngc tggggtcnng nnggnntacn nagg
//
