LOCUS       sequence-29625-e         644 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 644)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-CSNK1G1
FEATURES             Location/Qualifiers
     source          1..644
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    84..108
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(625..636)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ctcgggccca ataatgattt tatttttgac tgatagtgac ctgttcgttg caacaaattg
       61 atgagcaatg cttttttata atgccaactt tgtacaaaaa agttggcatg gtgctggagc
      121 tccttggccc tagcttggag gacttgtttg acctctgtga ccgaacattt actttgaaga
      181 cggtgttaat gatagccatc cagctgcttt ctcgaatgga atacgtgcac tcaaagaacc
      241 tcatttaccg agatgtcaag ccagagaact tcctgattgg tcgacaaggc aataagaaag
      301 agcatgttat acacattata gactttggac tggccaagga atacattgac cccgaaacca
      361 aaaaacacat accttatagg gaacacaaaa gtttaactgg aactgcaaga tatatgtcta
      421 tcaacacgca tcttggcaaa gagcaaagcc ggagagatga tttggaagcc ctaggccata
      481 tgttcatgta tttccttcga ggcagcctcc cctggcaagg actcaaggct gacacattaa
      541 aagagagata tcaaaaaatt ggtgacacca aaaggaatac tcccattgaa gctctctgtg
      601 agaactttcc agaggagatg gcaacctacc ttcgatatgt cagg
//
