LOCUS       sequence-296251-        9577 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAVS1 CMV-AtAFB2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9577)
  AUTHORS   Yesbolatova A, Saito Y, Kitamoto N, Makino-Itou H, Ajima R, Nakano 
            R, Nakaoka H, Fukui K, Gamo K, Tominari Y, Takeuchi H, Saga Y, 
            Hayashi KI, Kanemaki MT
  TITLE     The auxin-inducible degron 2 technology provides sharp degradation 
            control in yeast, mammalian cells, and mice.
  JOURNAL   Nat Commun. 2020 Nov 11;11(1):5701. doi: 10.1038/s41467-020-19532-z.
  PUBMED    33177522
REFERENCE   2  (bases 1 to 9577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-11-11"; volume: "11(1):5701. doi"; pages: " 
            10.1038/s41467-020-19532-z"
COMMENT     Sequence Label: pMK233 (AAVS1 CMV-AtAFB2)
FEATURES             Location/Qualifiers
     source          1..9577
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        242..260
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    295..1098
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     enhancer        1155..1458
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1459..1662
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1613..1633
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    3594..3715
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3631..3650)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3685..3704
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        3724..4224
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4143..4161
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4246..4845
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4246..4265)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4742..4762
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    4852..5076
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    5079..5915
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(5949..5968)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5950..5968)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5975..5992)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5975..5991)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5984..6006)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             6129..6431
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     6310..6329
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             6780..7574
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6780..7574
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6834..6853)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7444..7463
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(7824..8615,8616..8684))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8447..8466
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8808..9396
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9297..9316
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9550..9567
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca
      241 gaattaaccc tcactaaagg gactagtcct gcaggtttaa acgaattcgc cctttgcttt
      301 ctctgaccag cattctctcc cctgggcctg tgccgctttc tgtctgcagc ttgtggcctg
      361 ggtcacctct acggctggcc cagatccttc cctgccgcct ccttcaggtt ccgtcttcct
      421 ccactccctc ttccccttgc tctctgctgt gttgctgccc aaggatgctc tttccggagc
      481 acttccttct cggcgctgca ccacgtgatg tcctctgagc ggatcctccc cgtgtctggg
      541 tcctctccgg gcatctctcc tccctcaccc aaccccatgc cgtcttcact cgctgggttc
      601 ccttttcctt ctccttctgg ggcctgtgcc atctctcgtt tcttaggatg gccttctccg
      661 acggatgtct cccttgcgtc ccgcctcccc ttcttgtagg cctgcatcat caccgttttt
      721 ctggacaacc ccaaagtacc ccgtctccct ggctttagcc acctctccat cctcttgctt
      781 tctttgcctg gacaccccgt tctcctgtgg attcgggtca cctctcactc ctttcatttg
      841 ggcagctccc ctacccccct tacctctcta gtctgtgcta gctcttccag ccccctgtca
      901 tggcatcttc caggggtccg agagctcagc tagtcttctt cctccaaccc gggcccctat
      961 gtccacttca ggacagcatg tttgctgcct ccagggatcc tgtgtccccg agctgggacc
     1021 accttatatt cccagggccg gttaatgtgg ctctggttct gggtactttt atctgtcccc
     1081 tccaccccac agtggggcaa ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     1141 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     1201 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     1261 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     1321 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     1381 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     1441 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     1501 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     1561 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     1621 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag
     1681 atccgctagc gctgccacca tgaactattt tcctgacgag gtgattgagc acgtctttga
     1741 cttcgtgacc agccacaagg atcggaacgc tattagcctg gtgtgcaagt catggtacaa
     1801 gatcgagaga tactctaggc agaaagtctt cattggcaac tgttatgcta tcaaccccga
     1861 gcggttgctg cggcggttcc cctgcctgaa gtctctcacc ttgaagggga agccccactt
     1921 cgcagacttt aacctggtgc cacatgagtg gggcgggttt gtgctgccat ggattgaggc
     1981 cttggcccgg tccagggtgg ggctggagga gcttaggctc aagagaatgg tggtcaccga
     2041 tgagtccctg gagctgctca gcaggtcctt tgtcaacttc aagagcctcg tgctcgtcag
     2101 ctgcgagggg ttcaccactg atgggctggc atctatcgcc gctaactgta gacacctgag
     2161 ggatctcgat ctgcaggaaa atgaaatcga cgatcacaga gggcagtggt tgtcatgttt
     2221 ccccgacact tgcactaccc tggtcaccct taactttgcc tgcctggagg gcgaaaccaa
     2281 cctggtggcc ctcgaacggc tggtggcaag gagcccaaac ctcaaatctt tgaagctcaa
     2341 ccgggccgtc cccctggatg ctctggctag gttgatggcc tgcgcccctc agattgtgga
     2401 cctgggcgtc ggctcctacg agaatgaccc tgactccgag agctacctca agctgatggc
     2461 cgtgatcaag aaatgtacaa gcttgagaag cctgtcaggc tttctggaag ccgccccaca
     2521 ttgtctgtca gccttccacc ccatttgcca caatctcaca agcctgaacc tctcctacgc
     2581 cgctgagatc catggcagcc acctcatcaa actgattcag cactgcaaga agctgcagag
     2641 gttgtggatt ctggatagca tcggagacaa aggactggag gtggtggcct ccacctgcaa
     2701 agagctgcag gagctgaggg tgtttccctc cgacctgctc ggggggggga acacagccgt
     2761 gacagaggaa ggcctggtgg ccatctccgc cggatgcccc aagttgcact ccatcctgta
     2821 cttctgtcag cagatgacta acgccgctct tgtgacagtg gccaagaact gccccaactt
     2881 tattaggttc aggttgtgca tccttgagcc taacaagcct gaccacgtca caagtcagcc
     2941 cctggacgag ggcttcgggg caatcgtcaa ggcctgcaag tcccttaggc ggcttagcct
     3001 ctccggactg ctgaccgacc aggtcttcct ttacattggg atgtacgcca accaactgga
     3061 gatgctgagc attgcttttg ctggcgatac agataagggg atgctctacg tgctcaacgg
     3121 ctgcaagaaa atgaagaagt tggagatccg ggatagccct ttcggagata cagcccttct
     3181 cgctgatgtg agcaagtacg agactatgcg cagtctgtgg atgagtagtt gcgaggtgac
     3241 actgtccggc tgcaagcgcc tcgccgagaa ggccccctgg ctcaacgtgg agattatcaa
     3301 cgagaacgac aacaaccgca tggaggaaaa cggccatgaa ggacggcaga aggtcgataa
     3361 gctgtacctg tacagaaccg tcgtcgggac acggatggat gccccaccat ttgtgtggat
     3421 tttgtgagga tccgacgggc ccgtcgacgg taccgcgggc ccgggatcca ccggggccgc
     3481 gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     3541 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     3601 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     3661 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaagttg
     3721 gggttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg ctctgggcgt
     3781 ggttccggga aacgcagcgg cgccgaccct gggtctcgca cattcttcac gtccgttcgc
     3841 agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct tcctgctccg
     3901 cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc
     3961 gcacgtctca ctagtaccct cgcagacgga cagcgccagg gagcaatggc agcgcgccga
     4021 ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc gcgccgagag cagcggccgg
     4081 gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg
     4141 cggtgttccg cattctgcaa gcctccggag cgcacgtcgg cagtcggctc cctcgttgac
     4201 cgaatcaccg acctctctcc ccagggggat ccaccggagc ttaccatgac cgagtacaag
     4261 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     4321 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     4381 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     4441 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     4501 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     4561 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     4621 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     4681 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     4741 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     4801 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaggtac cctgtgcctt
     4861 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     4921 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     4981 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     5041 atagcaggca tgctggggat gcggtgggct ctatggttac tagggacagg attggtgaca
     5101 gaaaagcccc atccttaggc ctcctccttc ctagtctcct gatattgggt ctaaccccca
     5161 cctcctgtta ggcagattcc ttatctggtg acacaccccc atttcctgga gccatctctc
     5221 tccttgccag aacctctaag gtttgcttac gatggagcca gagaggatcc tgggagggag
     5281 agcttggcag ggggtgggag ggaagggggg gatgcgtgac ctgcccggtt ctcagtggcc
     5341 accctgcgct accctctccc agaacctgag ctgctctgac gcggctgtct ggtgcgtttc
     5401 actgatcctg gtgctgcagc ttccttacac ttcccaagag gagaagcagt ttggaaaaac
     5461 aaaatcagaa taagttggtc ctgagttcta actttggctc ttcacctttc tagtccccaa
     5521 tttatattgt tcctccgtgc gtcagtttta cctgtgagat aaggccagta gccagccccg
     5581 tcctggcagg gctgtggtga ggaggggggt gtccgtgtgg aaaactccct ttgtgagaat
     5641 ggtgcgtcct aggtgttcac caggtcgtgg ccgcctctac tccctttctc tttctccatc
     5701 cttctttcct taaagagtcc ccagtgctat ctgggacata ttcctccgcc cagagcaggg
     5761 tcccgcttcc ctaaggccct gctctgggct tctgggtttg agtccttggc aagcccagga
     5821 gaggcgctca ggcttccctg tcccccttcc tcgtccacca tctcatgccc ctggctctcc
     5881 tgccccttcc ctacaggggt tcctggctct gctctaaggg cgaattcgcg gccgctaaat
     5941 tcaattcgcc ctatagtgag tcgtattaca attcactggc cgtcgtttta caacgtcgtg
     6001 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     6061 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctat
     6121 acgtacggca gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg
     6181 atgtacagag tgatattatt gacacgccgg ggcgacggat ggtgatcccc ctggccagtg
     6241 cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg
     6301 aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag
     6361 aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct
     6421 ggggaatata aatgtcaggc atgagattat caaaaaggat cttcacctag atccttttca
     6481 cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta
     6541 tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat
     6601 ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg
     6661 cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa
     6721 ggatctgatg gcgcagggga tcaagctctg atcaagagac aggatgagga tcgtttcgca
     6781 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     6841 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     6901 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     6961 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     7021 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     7081 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     7141 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     7201 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     7261 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg
     7321 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     7381 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     7441 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     7501 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     7561 acgagttctt ctgaattatt aacgcttaca atttcctgat gcggtatttt ctccttacgc
     7621 atctgtgcgg tatttcacac cgcatcaggt ggcacttttc ggggaaatgt gcgcggaacc
     7681 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag attatcaaaa
     7741 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     7801 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     7861 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     7921 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     7981 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     8041 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     8101 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     8161 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     8221 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     8281 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     8341 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     8401 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     8461 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     8521 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     8581 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     8641 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     8701 tattattgaa gcatttatca gggttattgt ctcatgacca aaatccctta acgtgagttt
     8761 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     8821 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     8881 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     8941 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     9001 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     9061 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     9121 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     9181 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     9241 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     9301 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     9361 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     9421 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     9481 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     9541 accgagcgca gcgagtcagt gagcgaggaa gcggaag
//
