LOCUS       sequence-29628-e         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-CALM2
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    7..106
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             106..552
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPT
                     EAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAA
                     ELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     protein_bind    complement(554..653)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(670..689)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(671..689)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(694..710)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(694..710)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tcgggcccaa taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat
       61 gagcaatgct tttttataat gccaactttg tacaaaaaag ttggcatggc tgaccaactg
      121 actgaagagc agattgcaga attcaaagaa gctttttcac tatttgacaa agatggtgat
      181 ggaactataa caacaaagga attgggaact gtaatgagat ctcttgggca gaatcccaca
      241 gaagcagagt tacaggacat gattaatgaa gtagatgctg atggtaatgg cacaattgac
      301 ttccctgaat ttctgacaat gatggcaaga aaaatgaaag acacagacag tgaagaagaa
      361 attagagaag cattccgtgt gtttgataag gatggcaatg gctatattag tgctgcagaa
      421 cttcgccatg tgatgacaaa ccttggagag aagttaacag atgaagaagt tgatgaaatg
      481 atcagggaag cagatattga tggtgatggt caagtaaact atgaagagtt tgtacaaatg
      541 atgacagcaa agtgcccaac tttcttgtac aaagttggca ttataagaaa gcattgctta
      601 tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca
      661 gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg
      721 cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgcctc
      781 ct
//
