LOCUS       sequence-29633-e         614 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-PFKM
FEATURES             Location/Qualifiers
     source          1..614
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    72..96
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat gagcaatgct
       61 tttttataat gccaactttg tacaaaaaag ttggcatgac ccatgaagag caccatgcag
      121 ccaaaaccct ggggattggc aaagccattg ctgtcttaac ctctggtgga gatgcccaag
      181 gtatgaatgc tgctgtcagg gctgtggttc gagttggtat cttcaccggt gcccgtgtct
      241 tctttgtcca tgagggttat caaggcctgg tggatggtgg agatcacatc aaggaagcca
      301 cctgggagag cgtttcgatg atgcttcagc tgggaggcac ggtgattgga agtgcccggt
      361 gcaaggactt tcgggaacga gaaggacgac tccgagctgc ctacaacctg gtgaagcgtg
      421 ggatcaccaa tctctgtgtc attgggggtg atggcagcct cactggggct gacaccttcc
      481 gttctgagtg gagtgacttg ttgagtgacc tccagaaagc aggtaagatc acagatgagg
      541 aggctacgaa gtccagctac ctgaacattg tgggcctggt tgggtcaatt gacaatgact
      601 tctgtggcac cgat
//
