LOCUS       sequence-296391-       13475 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd Generation lentiviral vector expressing the codon-optimized 
            SARS-CoV2 Spike Glycoprotein ORF with a C-terminal HA tag generated 
            by Junko Ogawa.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13475)
  AUTHORS   Ogawa J, Zhu W, Tonnu N, Singer O, Hunter T, Ryan AL, Pao GM
  TITLE     The D614G mutation in the SARS-CoV2 Spike protein increases 
            infectivity in an ACE2 receptor dependent manner.
  JOURNAL   bioRxiv. 2020 Jul 22. doi: 10.1101/2020.07.21.214932.
  PUBMED    32743569
REFERENCE   2  (bases 1 to 13475)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv. 
            2020 Jul 22. doi: 10.1101/2020.07.21.214932."
COMMENT     Sequence Label: pBOB-CAG-SARS-CoV2-Spike-HA
FEATURES             Location/Qualifiers
     source          1..13475
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2157..2273
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2283..2662
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          2940..3956
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3880..3902
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3964..3983
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             7878..7904
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    8048..8636
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8101..8121)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8519..8530)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9043..9223
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9249..9266)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9255..9479
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9525..9953
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9612..9631)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9822..9843
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9962..9982)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9967..10296
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10147..10282
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10209..10228
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10344..10391
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10410..10784
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10914..11035
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10951..10970)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11005..11024
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11084..11100)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11084..11100)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11097..11119)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11108..11124
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11132..11138,11139..11156,11157..11162))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11177..11198
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11315..11332)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11486..12074)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11566..11585)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12245..13036,13037..13105))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12868..12887
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13106..13210)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(13285..13304)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcaactttt
     2161 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     2221 acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat tttatcgatg
     2281 tcgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
     2341 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     2401 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     2461 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     2521 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     2581 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     2641 attagtcatc gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat
     2701 ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc
     2761 gatgggggcg gggggggggg gggsgcgcgc crggsggggg gggggggggg gggggggggg
     2821 ggggggsgrg gsggrgaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
     2881 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
     2941 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
     3001 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
     3061 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
     3121 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg
     3181 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct
     3241 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg
     3301 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     3361 gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc
     3421 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg
     3481 gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg
     3541 ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg
     3601 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     3661 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
     3721 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
     3781 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
     3841 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     3901 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     3961 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc gctagtggat
     4021 ccaggccgcc tgggcccgtt aacaccatgt tcgtattcct tgtcctcctc cccctcgtca
     4081 gctcccagtg tgtcaatctg acaactcgaa ctcagctccc acccgcatac accaatagtt
     4141 ttacccgagg agtctattat cccgacaaag ttttcaggtc tagtgtgttg catagcacgc
     4201 aggacctgtt cttgccgttt ttttcaaatg tgacatggtt ccatgccatc catgtctctg
     4261 gtacgaacgg gaccaaaagg tttgataacc ccgttcttcc atttaacgac ggggtttatt
     4321 tcgcctctac tgaaaagtct aatataatac gaggttggat ttttgggaca acactggact
     4381 ctaaaacgca atctctcctc atagtaaata atgcgacgaa tgtcgtaatc aaagtatgtg
     4441 agtttcagtt ttgcaacgac ccttttcttg gtgtatacta tcacaagaac aacaagtctt
     4501 ggatggaaag tgaatttaga gtctacagct ccgcaaacaa ctgtaccttt gagtacgtca
     4561 gtcagccctt ccttatggac ctcgaaggca aacaggggaa cttcaagaat ctgcgcgagt
     4621 tcgtgttcaa gaatatcgac ggctatttca agatatattc aaaacataca ccaatcaatt
     4681 tggtacgcga tttgccgcag ggcttctctg ctcttgagcc tctcgtagat ttgcctatag
     4741 ggatcaatat tactcgcttt caaactctcc ttgcccttca tcggtcctac ctgacaccag
     4801 gcgacagctc cagtgggtgg acagctggtg cagcggctta ctacgtcggc tacctccaac
     4861 ccaggacttt tttgctgaaa tataatgaga atggtacgat tactgacgcc gtcgattgtg
     4921 ccctcgaccc attgagcgaa actaaatgca ccctgaaaag ctttacggtt gagaaaggga
     4981 tatatcagac atctaatttt cgcgttcaac ccacggaatc cattgtacga tttcccaaca
     5041 ttaccaattt gtgtcctttt ggtgaggtat ttaatgcaac cagattcgcg agtgtgtatg
     5101 cctggaatag gaaaagaatt tctaactgtg tagcagacta cagcgttctg tataatagtg
     5161 cgtcctttag tacgttcaag tgctacggag taagcccgac aaagttgaac gatttgtgtt
     5221 tcactaacgt ctatgccgat agcttcgtca tcaggggaga cgaagtaagg caaattgcac
     5281 caggccagac cggaaaaata gccgactaca actataagtt gccggacgat ttcaccgggt
     5341 gtgtcatcgc gtggaatagt aataacctgg actccaaggt gggcggcaac tacaactacc
     5401 tgtaccgatt gttccgcaag tctaacctca agccctttga acgagacata tccaccgaga
     5461 tataccaagc gggaagcact ccctgtaatg gcgttgaagg tttcaactgc tattttcctc
     5521 tccagtccta tggcttccaa cctaccaatg gagtaggcta tcaaccgtat cgagtcgtgg
     5581 tgctgagttt cgagctcctg cacgctccag cgaccgtttg tggtcctaag aaaagtacaa
     5641 acctcgtaaa aaataagtgc gtaaacttta actttaacgg tctcacagga acgggagttc
     5701 ttacagagtc caataagaag ttcctgccct ttcagcagtt tgggcgcgac atcgcggaca
     5761 ccacagatgc agtcagggac ccgcagactc ttgagatcct ggacatcacc ccatgctctt
     5821 ttggcggtgt gagtgtcatt acacccggga ccaacaccag caatcaggtt gctgtgctct
     5881 atcaagacgt caactgtacg gaagtcccag ttgcaataca cgcggaccag ttgacgccga
     5941 cgtggcgggt ttattctacc ggctcaaacg tctttcaaac tagagccggt tgtttgatcg
     6001 gggcggagca tgtgaacaat tcatacgaat gtgatattcc cataggagcc ggaatatgtg
     6061 cttcttacca gacccaaacc aactctccga gacgagcccg gtccgtagcc agtcaaagca
     6121 taattgcgta caccatgagc ctcggtgcag aaaactcagt tgcgtactca aataactcca
     6181 ttgccatccc aacaaatttt accatatcag tgaccactga aatccttcca gtgagcatga
     6241 ctaaaacaag tgtagattgc acaatgtata tttgcggaga ctcaactgag tgctctaacc
     6301 tcctgttgca gtacggttca ttctgtaccc agctcaatcg cgcacttacg ggaatagcag
     6361 tagaacaaga taagaatact caggaagtct tcgctcaagt aaagcaaatc tacaagacgc
     6421 cccccattaa ggactttggg ggttttaatt ttagccaaat actcccggac cccagtaaac
     6481 cctctaagag gtcatttata gaagacctgt tgtttaataa agttactctg gccgatgctg
     6541 gctttattaa acaatacggt gattgtttgg gcgacatcgc ggcgcgggat ctgatatgcg
     6601 cccaaaaatt taacggtctc actgtgttgc cgcctttgct cacagacgag atgatagctc
     6661 agtacacatc agcgttgttg gcgggtacca taacgtctgg atggacattc ggagcagggg
     6721 cggccttgca aataccattc gcaatgcaaa tggcatacag gttcaacggg ataggtgtta
     6781 cacagaatgt actttacgag aaccagaaat tgattgcaaa ccagtttaac tctgcgattg
     6841 gcaaaattca ggatagcctc agctccaccg cgtctgcact gggaaaactc caagatgttg
     6901 tgaaccaaaa tgcccaagcc ctcaatacac ttgtgaagca actctccagc aatttcggtg
     6961 caataagtag tgtgctgaac gacatacttt ctcggttgga caaagtagag gcggaggtgc
     7021 aaatcgacag actcataaca ggtcgactgc aatcattgca aacgtatgtt actcagcaat
     7081 tgatacgggc ggcggagatt cgagcgtctg ccaacctcgc cgcgactaaa atgtctgagt
     7141 gtgtgctcgg tcagtcaaaa cgcgtggact tctgtggaaa gggataccat ctcatgtcct
     7201 tccctcagtc tgcccctcac ggagtagtct ttcttcatgt gacctatgtt ccagcacagg
     7261 agaaaaattt taccacagcg cccgcgatat gccacgatgg caaagctcat tttccgcgag
     7321 agggagtatt tgtctctaac ggcacccatt ggtttgtaac ccagcgcaat ttttacgaac
     7381 ctcagataat caccactgac aacacctttg ttagtggtaa ttgtgatgta gtgatcggga
     7441 tagtgaacaa tacagtgtac gaccccctgc aacctgagct ggactccttt aaagaagaac
     7501 tcgacaagta cttcaaaaac cacacttccc cggatgtcga tttgggtgat atatctggca
     7561 tcaatgcgag tgtagtcaac atacagaagg aaatcgacag gctcaatgaa gtagcaaaaa
     7621 acctgaatga atccctcata gacctccaag agctgggcaa atatgagcag tacattaaat
     7681 ggccctggta catttggctg ggcttcatcg ctggcctgat agcgatagtg atggttacta
     7741 ttatgctctg ctgtatgacg agttgttgca gctgcctcaa agggtgttgt tcttgcggct
     7801 cctgttgtaa gttcgacgag gatgattcag agcccgttct taagggtgta aaactccatt
     7861 acactggagg aggaggttac ccatacgatg ttccagatta cgcttagtaa actagtaacg
     7921 tctcgagttt aaacctggaa aaacatggag caatcacaag tagcaataca gcagctacca
     7981 atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac
     8041 ctcagacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     8101 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     8161 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     8221 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     8281 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     8341 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     8401 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
     8461 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     8521 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     8581 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg
     8641 aattcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     8701 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatatc
     8761 cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa ctacacacca
     8821 gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
     8881 caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca ccctgtgagc
     8941 ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga cagccgccta
     9001 gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct ctggttagac
     9061 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     9121 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     9181 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagggccgc tttaaacccg
     9241 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     9301 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     9361 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     9421 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     9481 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
     9541 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     9601 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     9661 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     9721 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     9781 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     9841 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     9901 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
     9961 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
    10021 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
    10081 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
    10141 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
    10201 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
    10261 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
    10321 attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag
    10381 tataatacga caaggtgagg aactaaacca tggccaagtt gaccagtgcc gttccggtgc
    10441 tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc gggttctccc
    10501 gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc ctgttcatca
    10561 gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc
    10621 tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg gacgcctccg
    10681 ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc
    10741 cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg ctacgagatt
    10801 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
    10861 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
    10921 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
    10981 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
    11041 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
    11101 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
    11161 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
    11221 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
    11281 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
    11341 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
    11401 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
    11461 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
    11521 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
    11581 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
    11641 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
    11701 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
    11761 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
    11821 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
    11881 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
    11941 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
    12001 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
    12061 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
    12121 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
    12181 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
    12241 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
    12301 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
    12361 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
    12421 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
    12481 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
    12541 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
    12601 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
    12661 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    12721 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    12781 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    12841 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    12901 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    12961 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    13021 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    13081 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    13141 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    13201 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc
    13261 cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
    13321 tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac
    13381 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc
    13441 gctgcttcgc gatgtacggg ccagatatac gcgtt
//
