LOCUS       sequence-296393-        3910 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of NSP1 gene's CDS from
            SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 3910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR207 SARS-CoV-2 NSP1_nostop
FEATURES             Location/Qualifiers
     source          1..3910
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(56..142)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    195..294
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(835..934)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1046..1065)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1124..1143)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(1717..2250)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(2439..2444,2445..2461,2462..2467))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      3124..3712
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3613..3632
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3847..3874)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg
       61 tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg
      121 actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgctagca
      181 tggatctcgg gccccaaata atgattttat tttgactgat agtgacctgt tcgttgcaac
      241 aaattgatga gcaatgcttt tttataatgc caactttgta caaaaaagtt ggcatggaga
      301 gcctggtgcc tggcttcaac gaaaagaccc acgtgcagct gtctctgcca gtgctgcagg
      361 tgcgcgacgt gctggtgagg ggattcggtg actctgtgga ggaagtgctg tctgaagcta
      421 gacagcacct gaaggatggt acctgcggac tggtggaggt ggaaaagggc gtgctgcccc
      481 agctggagca gccttacgtg ttcatcaagc gcagcgatgc taggactgct ccccacggac
      541 acgtgatggt ggaactggtg gccgagctgg aaggtatcca gtacggtcgc tccggagaga
      601 ccctgggtgt gctggtgcca cacgtgggtg aaatccccgt ggcttacaga aaggtgctgc
      661 tgcgtaagaa cggaaacaag ggtgccggcg gacactccta cggtgctgac ctgaagtctt
      721 tcgacctggg cgacgagctg ggaactgacc cttacgagga cttccaggaa aactggaaca
      781 ccaagcacag ctccggagtg accagagagc tgatgcgtga actgaacggt ggctacccaa
      841 ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac
      901 aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgcagct ctggcccgtg
      961 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa
     1021 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg
     1081 tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
     1141 gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca
     1201 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
     1261 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
     1321 cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta
     1381 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg
     1441 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct
     1501 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt
     1561 aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg
     1621 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa
     1681 ttaggccggg aagccgatct cggcttgaac gaattgttag gtggcggtac ttgggtcgat
     1741 atcaaagtgc atcacttctt cccgtatgcc caactttgta tagagagcca ctgcgggatc
     1801 gtcaccgtaa tctgcttgca cgtagatcac ataagcacca agcgcgttgg cctcatgctt
     1861 gaggagattg atgagcgcgg tggcaatgcc ctgcctccgg tgctcgccgg agactgcgag
     1921 atcatagata tagatctcac tacgcggctg ctcaaacttg ggcagaacgt aagccgcgag
     1981 agcgccaaca accgcttctt ggtcgaaggc agcaagcgcg atgaatgtct tactacggag
     2041 caagttcccg aggtaatcgg agtccggctg atgttgggag taggtggcta cgtctccgaa
     2101 ctcacgaccg aaaagatcaa gagcagcccg catggatttg acttggtcag ggccgagcct
     2161 acatgtgcga atgatgccca tacttgagcc acctaacttt gttttagggc gactgccctg
     2221 ctgcgtaaca tcgttgctgc tgcgtaacat cgttgctgct ccataacatc aaacatcgac
     2281 ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtaca aaaaaacagt
     2341 cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc
     2401 tggaccagtt gcgtgagcgc atacgctact tgcattacag tttacgaacc gaacaggctt
     2461 atgtcaactg ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg
     2521 gcagcagcga agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct
     2581 ccacgcatcg tcaggcattg gcggccttgc tgttcttcta cggcaaggtg ctgtgcacgg
     2641 atctgccctg gcttcaggag atcggaagac ctcggccgtc gcggcgcttg ccggtggtgc
     2701 tgaccccgga tgaagtggtt cgcatcctcg gttttctgga aggcgagcat cgtttgttcg
     2761 cccaggactc tagctatagt tctagtggtt ggctactaat aggttgtatt gatgttggac
     2821 gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt
     2881 tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga
     2941 ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt
     3001 tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat
     3061 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     3121 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     3181 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3241 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     3301 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     3361 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3421 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     3481 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     3541 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     3601 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     3661 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     3721 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     3781 tgcgttatcc cctgattctg tggataaccg tattaccgct agccaggaag agtttgtaga
     3841 aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat
     3901 ggcgggcgtc
//
