LOCUS       sequence-296398-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Backbone for cloning LwCas13a guides under U6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Abudayyeh OO, Gootenberg JS, Essletzbichler P, Han S, Joung J, 
            Belanto JJ, Verdine V, Cox DBT, Kellner MJ, Regev A, Lander ES, 
            Voytas DF, Ting AY, Zhang F
  TITLE     RNA targeting with CRISPR-Cas13.
  JOURNAL   Nature. 2017 Oct 4. doi: 10.1038/nature24049.
  PUBMED    28976959
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2017 Oct 4. doi: 10.1038/nature24049."
COMMENT     Sequence Label: pC016 - LwCas13a guide expression backbone wit...
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     288..307
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        450..698
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     450..470
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     621..640
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
       61 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
      121 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
      181 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
      241 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
      301 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
      361 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
      421 gccttttgct ggccttttgc tcagctagcg agggcctatt tcccatgatt ccttcatatt
      481 tgcatatacg atacaaggct gttagagaga taattggaat taatttgact gtaaacacaa
      541 agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt
      601 taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt
      661 tcttggcttt atatatcttg tggaaaggac gaaacaccga tttagactac cccaaaaacg
      721 aaggggacta aaacgggtct tcgagaagac cttttttttg aattctgatg cggtattttc
      781 tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat
//
