LOCUS       sequence-296405-        9473 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression plasmid for AapCas12b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9473)
  AUTHORS   Joung J, Ladha A, Saito M, Segel M, Bruneau R, Huang MW, Kim NG, Yu 
            X, Li J, Walker BD, Greninger AL, Jerome KR, Gootenberg JS, 
            Abudayyeh OO, Zhang F
  TITLE     Point-of-care testing for COVID-19 using SHERLOCK diagnostics.
  JOURNAL   medRxiv. 2020 May 8. doi: 10.1101/2020.05.04.20091231.
  PUBMED    32511521
REFERENCE   2  (bases 1 to 9473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "medRxiv. 
            2020 May 8. doi: 10.1101/2020.05.04.20091231."
COMMENT     Sequence Label: pAG001 His6-TwinStrep-SUMO-AapCas12b
FEATURES             Location/Qualifiers
     source          1..9473
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      77..665
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     566..585
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     819..836
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    851..990
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(916..935)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1076..1098
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1092..1283)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(2595..3677)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     3639..3658
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(3678..3755)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     3961..3980
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     4064..4083
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4064..4082
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4083..4107
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             4122..4144
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4163..4180
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4190..4207
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             join(4220..4243,4244..4279,4280..4303)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             4304..4597
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
     primer_bind     complement(8037..8055)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      8051..8098
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(8273..8278,8279..8295,8296..8301))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(8328..8346)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8414..8518
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8519..8587,8588..9379)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8737..8756)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
       61 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
      121 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      181 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
      241 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      301 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      361 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      421 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      481 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      541 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      601 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      661 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      721 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
      781 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
      841 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
      901 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg
      961 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg
     1021 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     1081 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa
     1141 agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc
     1201 tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg
     1261 gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg
     1321 gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat
     1381 gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag
     1441 agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag
     1501 ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc
     1561 cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc
     1621 gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc
     1681 taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg
     1741 cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg
     1801 gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag
     1861 aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca
     1921 ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt
     1981 atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa
     2041 tcgccgtgac gatcagcggt ccaatgatcg aagttaggct ggtaagagcc gcgagcgatc
     2101 cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg
     2161 gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg
     2221 tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct
     2281 gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca
     2341 agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct
     2401 cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga
     2461 cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt
     2521 tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat
     2581 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     2641 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt
     2701 cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt
     2761 tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt
     2821 aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc
     2881 gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg
     2941 ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga
     3001 aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga
     3061 gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc
     3121 gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta
     3181 ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat
     3241 aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga
     3301 tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag
     3361 gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg
     3421 cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca
     3481 acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa
     3541 ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc
     3601 tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat
     3661 aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc
     3721 ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg
     3781 cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc
     3841 aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac
     3901 catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc
     3961 ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac
     4021 gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata
     4081 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
     4141 gagatatacc atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg
     4201 cggcagccat atggctagct ggagccatcc gcagtttgaa aaaggtggtg gtagcggtgg
     4261 tggttcaggt ggtagtgcat ggtcacaccc tcagtttgag aaaatgtcgg actcagaagt
     4321 caatcaagaa gctaagccag aggtcaagcc agaagtcaag cctgagactc acatcaattt
     4381 aaaggtgtcc gatggatctt cagagatctt cttcaagatc aaaaagacca ctcctttaag
     4441 aaggctgatg gaagcgttcg ctaaaagaca gggtaaggaa atggactcct taagattctt
     4501 gtacgacggt attagaatcc aagctgatca gacccctgaa gatttggaca tggaggataa
     4561 cgatattatt gaggctcaca gagaacagat tggtggatcc atggccgtaa aatctatgaa
     4621 agttaagttg cgtctggaca acatgccaga aattcgcgct ggcctttgga aactgcacac
     4681 cgaggtcaat gcaggcgttc gttattatac cgagtggtta agtttattgc gtcaggaaaa
     4741 tttataccgt cgcagtccaa acggggacgg ggaacaggag tgttacaaga cagctgagga
     4801 gtgcaaggca gagttgcttg agcgcttgcg cgcacgccaa gttgagaatg gacactgcgg
     4861 tccggctggg tcagacgatg agcttcttca attggcacgt cagttatacg aattattagt
     4921 accccaggcg atcggtgcga aaggcgacgc tcagcagatc gcgcgtaaat tcctgtcccc
     4981 gctggctgat aaagatgctg tcggtggcct gggaattgcc aaagccggga ataaaccgcg
     5041 ttgggtccgt atgcgcgagg ctggagagcc gggatgggaa gaagagaagg caaaagcgga
     5101 agcccgtaaa tccacggacc gtactgcaga cgtcttgcgc gccttagccg attttgggct
     5161 taaaccgttg atgcgcgtct acaccgactc agatatgtcg tctgttcaat ggaagcccct
     5221 gcgtaaaggg caggccgtac gtacctggga tcgtgatatg tttcagcagg cgatcgagcg
     5281 tatgatgtcc tgggaatcct ggaaccaacg cgtaggcgaa gcatacgcaa aattggtcga
     5341 acaaaagtcg cgcttcgagc agaagaactt tgtcggacag gaacatctgg ttcaattggt
     5401 caaccagctg caacaggaca tgaaggaagc atctcacggt ctggaatcga aagagcaaac
     5461 tgcacactat cttactggtc gtgcgttgcg tggtagtgat aaagtgtttg agaagtggga
     5521 gaagcttgat cccgatgcgc cttttgatct ttacgacacc gagattaaga acgttcaacg
     5581 tcgcaataca cgtcgctttg gttcgcatga cttattcgcc aagttggcag aaccgaaata
     5641 ccaagccctg tggcgtgaag acgcgagctt tcttacgcgt tatgcagtgt acaatagtat
     5701 tgtccgcaaa ctgaaccacg ccaaaatgtt tgcaaccttt acgcttccag acgccacagc
     5761 tcaccctatt tggacccgct ttgataaatt ggggggtaat cttcaccaat atacctttct
     5821 gttcaacgag ttcggtgagg gtcgccatgc tatccgcttc caaaagttac tgacggtgga
     5881 agacggcgtg gcaaaggaag ttgatgacgt gactgtcccg atcagcatgt cagcccagtt
     5941 agacgacctg ctgccgcgcg atccgcatga attggtcgcg ctttatttcc aagattatgg
     6001 cgcggaacaa cacttagcgg gtgaattcgg tggggcaaaa atccaatatc gtcgcgacca
     6061 gcttaaccac ctgcacgctc gccgcggtgc ccgcgatgtt tatctgaacc tttcggtacg
     6121 tgtgcagagt caatcagagg cacgcggcga acgtcgtcct ccatatgctg cagtctttcg
     6181 cttggtaggg gataatcacc gcgcatttgt gcatttcgat aagttatctg actaccttgc
     6241 agaacatccg gatgatggta aattgggctc ggagggtctt ttatcaggct tacgcgtaat
     6301 gagtgtagac ctgggcctgc gtacttcagc cagcatctct gtatttcgcg tcgcacgtaa
     6361 agacgagtta aaaccgaatt ccgagggtcg cgttcctttc tgcttcccta ttgaagggaa
     6421 cgagaacctg gtagcggttc acgaacgctc ccaattgttg aagctgcccg gagagacaga
     6481 atcgaaggat ctgcgtgcta tccgtgagga gcgccagcgt acgttgcgcc aattacgtac
     6541 acagcttgca tatttgcgtc ttttagtacg ctgtggttca gaggatgtgg gtcgccgtga
     6601 acgttcctgg gcaaagctga tcgaacaacc gatggatgct aatcagatga cccccgactg
     6661 gcgcgaagct ttcgaggacg agctgcagaa acttaagtca ttgtatggta tctgtggaga
     6721 tcgtgaatgg actgaggcag tctacgaatc tgtgcgccgc gtatggcgcc atatgggaaa
     6781 gcaggtacgc gactggcgta aggatgttcg ttccggcgaa cgccctaaga ttcgcggcta
     6841 tcagaaagac gttgttggtg gcaattcgat tgaacagatt gagtatcttg aacgtcagta
     6901 caaatttctg aagtcttggt cattctttgg aaaagtatca ggtcaggtaa ttcgtgccga
     6961 gaaggggtcc cgcttcgcta tcaccttacg cgaacatatc gatcatgcga aagaagaccg
     7021 ccttaaaaaa ttggctgatc gtatcatcat ggaggcatta ggatatgtgt atgcactgga
     7081 cgacgaacgt ggcaaaggga aatgggttgc caagtatccg ccgtgtcaat tgattttact
     7141 ggaagaattg tcggagtacc agtttaacaa cgaccgtccc ccctcagaga ataaccagct
     7201 tatgcaatgg tcgcatcgtg gagtgttcca agaactgtta aaccaagcac aagttcatga
     7261 ccttcttgta ggtactatgt atgcggcatt ttccagtcgc tttgacgcac gtacgggggc
     7321 tccggggatc cgttgtcgtc gcgtacctgc acgttgcgca cgcgaacaga atccggagcc
     7381 ttttccttgg tggttgaata agttcgttgc ggagcataag ttggacggct gccctttgcg
     7441 cgctgatgac ctgatcccca caggcgaagg tgagttcttc gttagtccat tttcagctga
     7501 ggagggtgac tttcaccaga tccatgccga ccttaacgcc gcccaaaatt tgcaacgtcg
     7561 tctttggtca gactttgata tctcgcaaat tcgtctgcgc tgtgattggg gggaggttga
     7621 tggcgagcca gttttaattc cgcgcacaac tggcaagcgt accgctgaca gctacggcaa
     7681 caaggttttt tataccaaga ccggtgtaac gtattacgaa cgtgagcgcg gtaaaaaacg
     7741 tcgcaaagtt tttgctcagg aagaactgag tgaagaagag gctgaacttt tggtggaagc
     7801 cgatgaggcc cgtgagaaat cagtagtttt gatgcgtgat ccgtcaggaa tcattaatcg
     7861 tggagactgg acgcgtcaaa aagaattttg gagtatggtc aaccaacgca tcgagggtta
     7921 ccttgttaag cagatccgct cgcgcgtccg tctgcaggaa agtgcttgtg agaacactgg
     7981 ggacatctga gcggccgcac tcgaggcccg aaaggaagct gagttggctg ctgccaccgc
     8041 tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct
     8101 gaaaggagga actatatccg gatatcccgc aagaggcccg gcagtaccgg cataaccaag
     8161 cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat
     8221 ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc
     8281 ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg
     8341 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     8401 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     8461 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     8521 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     8581 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     8641 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     8701 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     8761 tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     8821 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     8881 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     8941 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     9001 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     9061 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     9121 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     9181 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     9241 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     9301 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     9361 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     9421 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atc
//
