LOCUS       sequence-296414-        2809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Truncated mouse gamma-synuclein (mSncg-0.66kb) promoter-mediates 
            gene expression in retinal ganglion cells with fluorescent reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Wang Q, Zhuang PS, Huang H, Li L, Liu L, Webber HC, Dalal R, Siew L,
            Fligor CM, Chang KC, Nahmou M, Kreymerman A, Sun Y, Meyer JS, 
            Goldberg JL, Hu Y
  TITLE     Mouse gamma-Synuclein Promoter-Mediated Gene Expression and Editing 
            in Mammalian Retinal Ganglion Cells.
  JOURNAL   J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0102-20.2020. doi: 
            10.1523/JNEUROSCI.0102-20.2020.
  PUBMED    32300046
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2020 Apr 9. pii: JNEUROSCI.0102-20.2020. doi: 
            10.1523/JNEUROSCI.0102-20.2020."
COMMENT     Sequence Label: pAM-AAV-mSncg-0.66kb-EGFP
FEATURES             Location/Qualifiers
     source          1..2809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   1..130
                     /label=AAV2 ITR
     CDS             join(972..974,975..977,978..1691)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(972..974,975..977,978..1688)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1017..1038)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1278..1297)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1625..1646
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1749..2337
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1802..1822)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2220..2231)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2364..2381)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2370..2594
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2680..2809
                     /label=AAV2 ITR
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatttacgta gccatgctct
      181 aggtaccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      241 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      301 atcatatgct atgggaacta gcaggtgggt gggaactcag agaaggaaag ggactatgct
      361 agaatcacac agcgggcagc ccagtctagg gcatggggag cagctttggg tgtttctggc
      421 ctcagccttc caacaggttt ggctagagct ccaggctcaa gagcatccag gatacagtgg
      481 ggaactggat aacagggcag cctgcaggtt ggccattcat tggattggcc ctgaccccgg
      541 cccagcctgg gacactgagg catcatcagt caaggcactt ttcttctgca tataagagcc
      601 agggcacgag accaccaggg ctttccaagg atgaatgagg tgtaatgata gattaggata
      661 tgtccagcct ccaacacgct ctccctcccc cagggccaac aagagtcagc agggcagaat
      721 agagccagta ggggcccggg ccctgctcgc tggtatcccc gtgaggcatg ccttctctct
      781 ggcccgccct ccctgccccc accctggccc gggctggctg ggctccagcc agcagccaca
      841 gcatcaatat ttcatctgcg tcaataagag gcagtagcag cagagacagc ggctgcggca
      901 gcactccagt ccatagcttg cagcagccag gttccatcct tgcaaacacc atggacgtct
      961 tcaaggaatt catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     1021 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     1081 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     1141 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     1201 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     1261 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     1321 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     1381 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     1441 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     1501 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     1561 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     1621 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     1681 tgtacaagta aagatctacg cgtggtacct ctagagtcga cccgggcggc ctcgagaagc
     1741 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     1801 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     1861 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     1921 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     1981 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     2041 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     2101 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     2161 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     2221 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     2281 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
     2341 gataccgtcg actcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     2401 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     2461 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     2521 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     2581 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctcgacta gagcatggct
     2641 acgtagataa gtagcatggc gggttaatca ttaactacaa ggaaccccta gtgatggagt
     2701 tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc
     2761 gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcag
//
