LOCUS       sequence-296437-        3711 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of NSP5 gene's CDS from
            SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 3711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR223 SARS-CoV-2 NSP5_nostop
FEATURES             Location/Qualifiers
     source          1..3711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(118..145)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(237..323)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     372..394
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     386..403
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     387..403
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    419..518
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1440..1539)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1556..1575)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1557..1575)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1580..1596)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1580..1596)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2027..2818
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2911..3499
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3400..3419
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3653..3670
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
       61 tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa
      121 aggccatccg tcaggatggc cttctgctta gtttgatgcc tggcagttta tggcgggcgt
      181 cctgcccgcc accctccggg ccgttgcttc acaacgttca aatccgctcc cggcggattt
      241 gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtcttcc
      301 gactgagcct ttcgttttat ttgatgcctg gcagttccct actctcgcgt taacgctagc
      361 atggatgttt tcccagtcac gacgttgtaa aacgacggcc agtcttaagc tcgggcccca
      421 aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg atgagcaatg
      481 cttttttata atgccaactt tgtacaaaaa agttggcatg tccggattcc gcaagatggc
      541 cttcccttct ggcaaggtgg agggctgcat ggtgcaggtg acctgcggaa ccaccaccct
      601 gaacggtctg tggctggacg acgtggtgta ctgccccagg cacgtgatct gcacctctga
      661 ggacatgctg aaccctaact acgaagacct gctgatcaga aagagcaacc acaacttcct
      721 ggtgcaggcc ggtaacgtgc agctgcgtgt gatcggccac tctatgcaga actgcgtgct
      781 gaagctgaag gtggacaccg ctaaccccaa gacccctaag tacaagttcg tgagaatcca
      841 gcctggtcag accttctccg tgctggcctg ctacaacgga agcccatccg gcgtgtacca
      901 gtgcgctatg cgtcccaact tcaccatcaa gggcagcttc ctgaacggct cttgcggaag
      961 cgtgggtttc aacatcgact acgactgcgt gtccttctgc tacatgcacc acatggagct
     1021 gccaactgga gtgcacgctg gaactgacct ggagggtaac ttctacggcc ccttcgtgga
     1081 cagacagact gctcaggctg ctggcaccga caccaccatc accgtgaacg tgctggcttg
     1141 gctgtacgcc gctgtgatca acggagaccg ctggttcctg aacaggttca ccaccaccct
     1201 gaacgacttc aacctggtgg ctatgaagta caactacgag cctctgaccc aggaccacgt
     1261 ggatatcctg ggtccactga gcgcccagac tggaatcgct gtgctggaca tgtgcgcctc
     1321 cctgaaggaa ctgctgcaga acggcatgaa cggacgcacc atcctgggat ccgctctgct
     1381 ggaggacgaa ttcaccccct tcgacgtggt gaggcagtgc tctggtgtga ccttccagta
     1441 cccaactttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa
     1501 cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg atatccccta
     1561 tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa
     1621 tctctgatgt tacattgcac aagataaaaa tatatcatca tgcctcctct agaccagcca
     1681 ggacagaaat gcctcgactt cgctgctacc caaggttgcc gggtgacgca caccgtggaa
     1741 acggatgaag gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa
     1801 gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg
     1861 cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg
     1921 ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa
     1981 cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacattatga gggaagcggt
     2041 gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga
     2101 accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca
     2161 cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc
     2221 tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc
     2281 tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg
     2341 cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc
     2401 cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt
     2461 ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc
     2521 gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa
     2581 tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa
     2641 ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact
     2701 tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca
     2761 gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataacc
     2821 ctcgagccac ccatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc
     2881 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     2941 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     3001 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     3061 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     3121 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     3181 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     3241 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     3301 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     3361 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     3421 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     3481 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     3541 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     3601 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     3661 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg c
//
