LOCUS       sequence-296444-        3132 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of NSP9 gene's CDS from
            SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR223 SARS-CoV-2 NSP9_nostop
FEATURES             Location/Qualifiers
     source          1..3132
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(138..165)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(257..343)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     392..414
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     406..423
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     407..423
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    439..538
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(881..980)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(997..1016)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(998..1016)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1021..1037)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1021..1037)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1468..2259
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2352..2940
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2841..2860
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3094..3111
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
       61 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
      121 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
      181 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
      241 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
      301 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
      361 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
      421 agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac ctgttcgttg
      481 caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa agttggcatg
      541 aacaacgagc tgagccctgt ggccctgaga cagatgtctt gtgctgctgg taccacccag
      601 accgcttgca ccgacgacaa cgccctggct tactacaaca ccaccaaggg cggaagattc
      661 gtgctggccc tgctgtctga cctgcaggac ctgaagtggg ctcgtttccc caagagcgac
      721 ggtaccggca ccatctacac cgagctggaa cccccttgca gattcgtgac cgacacccca
      781 aagggcccca aggtgaagta cctgtacttc atcaagggac tgaacaacct gaacagagga
      841 atggtgctgg gtagcctggc tgctaccgtg aggctgcagt acccaacttt cttgtacaaa
      901 gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt
      961 caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg
     1021 gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca
     1081 caagataaaa atatatcatc atgcctcctc tagaccagcc aggacagaaa tgcctcgact
     1141 tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc
     1201 cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc
     1261 aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg
     1321 gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg
     1381 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc
     1441 agtcgcccta aaacaaagtt aaacattatg agggaagcgg tgatcgccga agtatcgact
     1501 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
     1561 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg
     1621 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
     1681 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt
     1741 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
     1801 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg
     1861 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
     1921 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
     1981 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc
     2041 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
     2101 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat
     2161 cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac
     2221 tacgtgaaag gcgagatcac caaggtagtc ggcaaataac cctcgagcca cccatgacca
     2281 aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc
     2341 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     2401 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     2461 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     2521 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     2581 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     2641 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     2701 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     2761 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     2821 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     2881 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     2941 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3001 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     3061 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     3121 gagcgcccaa ta
//
