LOCUS       sequence-296451-        3639 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with a fragmented insert of S 
            gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR221 SARS-CoV-2 S-frag1_nostop
FEATURES             Location/Qualifiers
     source          1..3639
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgttcgtgt tcctggtgct gctgccactg gtgagctccc agtgcgtgaa cctgaccacc
       61 agaacccagc tgccacctgc ctacaccaac tccttcacca ggggcgtgta ctacccagac
      121 aaggtgttca gatccagcgt gctgcactct acccaggacc tgttcctgcc attcttctct
      181 aacgtgacct ggttccacgc catccacgtg tctggaacca acggcaccaa gagattcgac
      241 aacccagtgc tgccattcaa cgacggcgtg tacttcgctt ctaccgagaa gtctaacatc
      301 atcaggggtt ggatcttcgg aaccaccctg gactctaaga cccagtctct gctgatcgtg
      361 aacaacgcta ccaacgtggt gatcaaggtg tgcgagttcc agttctgcaa cgacccattc
      421 ctgggagtgt actaccacaa gaacaacaag tcttggatgg aaagcgagtt cagggtgtac
      481 agctctgcta acaactgcac cttcgagtac gtgtctcagc ctttcctgat ggacctggaa
      541 ggaaagcagg gaaacttcaa gaacctgaga gagttcgtgt tcaagaacat cgacggttac
      601 ttcaagatat acagcaagca caccccaatc aacctggtga gggacctgcc acagggtttc
      661 tctgctctgg agcccctggt ggacctgcca atcggcatca acatcaccag gttccagacc
      721 ctgctggctc tgcacaggtc ttacctgacc ccaggcgaca gcagctctgg atggaccgcc
      781 ggcgccgctg cttactacgt gggatacctg cagccaagga ccttcctgct gaagtacaac
      841 gaaaacggca ccatcaccga cgccgtggac tgcgctctgg acccactgtc tgagaccaag
      901 tgcaccctga agtctttcac cgtggaaaag ggaatctacc agaccagcaa cttccgcgtg
      961 cagcccaccg agtctatcgt gagattccca aacatcacca acctgtgccc attcggagaa
     1021 gtgttcaacg ctaccaggtt cgccagcgtg tacgcttgga acaggaagag aatcagcaac
     1081 tgcgtggccg actactctgt gctgtacaac tctgcctcct tctccacctt caagtgctac
     1141 ggcgtgtccc caaccaagct gaacgacctg tgcttcacca acgtgtacgc tgactccttc
     1201 gtgatcagag gagacgaagt gagacagatc gctcccggtc agaccggaaa gatcgccgac
     1261 tacaactaca agctgccaga cgacttcacc ggttgcgtga tcgcttggaa ctctaacaac
     1321 ctggactcta aggtgggagg caactacaac tacctgtaca ggctgttccg caagagcaac
     1381 ctgaagcctt tcgaaaggga catcagcacc gaaatctacc aggctggatc taccccatgc
     1441 aacggagtgg aaggattcaa ctgctacttc cctctgcagt cttacggatt ccagccaacc
     1501 aacggagtgg gataccagcc atacagagtg gtggtgctgt ctttcgagct gctgcacgct
     1561 cctgctaccg tgtgcggacc aaagaagtct accaacctgg tgaagaacaa gtgcgtgaac
     1621 ttcaacttca acggactgac cggaaccgga gtgctgaccg aatccaacaa gaagttcctg
     1681 cctttccagc agttcggtag agacatcgcc gacaccaccg acgctgtgag agacccacag
     1741 accctggaaa tcctggacat caccccctgc tctttcggag gagtgagcgt gatcacccca
     1801 ggaaccaaca cctccaacca ggtggccgtg ctgtaccagg acgtgaactg caccgaagtg
     1861 ccagtggcca tccacgctga ccagctgacc ccaacctgga gagtgtacag caccggatct
     1921 aacgtgttcc agacccgtgc cggctgcctg atcggagccg agcacgtgaa caactcctac
     1981 gaatgcgaca tcccaatcgg agctggcatc tgcgcttctt accagaccca gaccaacagc
     2041 ccaagaaggg ccagatccgt ggcctctcag agcatcatcg cctacaccat gagcctgggc
     2101 gctgaaaaca gcgtggctta cagcaacaac tctatcgcca tcccaaccaa cttcaccatc
     2161 tctgtgacca ccgaaatcct gccagtgagc atgaccaaga ccagcgtgga ctgcaccatg
     2221 tacatctgcg gagactctac cgaatgctct aacctgctgc tgcagtacgg cagcttctgc
     2281 acccagctga accgcgctct gaccggaatc gctgtggagc aggacaagaa cacccaggaa
     2341 gtgttcgctc aggtgaagca gatatacaag accccaccta tcaaggactt cggaggattc
     2401 aacttctctc agatcctgcc cgacccatct aagccctcta agaggtcttt catcgaagac
     2461 ctgctgttca acaaggtgac cctggccgac gccggtttca tcaagcagta cggtgactgc
     2521 ctgggagaca tcgccgctag agacctgatc tgcgcccaga agttcaacgg tctgaccgtg
     2581 ctgccaccac tgctgaccga cgagatgatc gcccagtaca cctctgccct gctggctgga
     2641 accatcacct ctggttggac cttcggagct ggcgccgccc tgcagatccc attcgctatg
     2701 cagatggcct accgtttcaa cggtatcgga gtgacccaga acgtgctgta cgaaaaccag
     2761 aagctgatcg ctaaccagtt caactctgct atcggaaaga tccaggactc cctgtcctct
     2821 accgcttctg ccctgggaaa gctgcaggac gtggtgaacc agaacgccca ggccctgaac
     2881 accctggtga agcagctgtc ttctaacttc ggagccatct cctctgtgct gaacgacatc
     2941 ctgtctaggc tggacaaggt ggaagccgag gtgcagatcg acagactgat caccggaaga
     3001 ctgcagtctc tgcagaccta cgtgacccag cagctgatca gggccgctga aatcagagct
     3061 tctgccaacc tggctgccac caagatgtcc gaatgcgtgc tgggccagtc taagagggtg
     3121 gacttctgcg gaaagggata ccacctgatg tccttcccac agtctgctcc acacggagtg
     3181 gtgttcctgc acgtgaccta cgtgcccgcc caggaaaaga acttcaccac cgccccagct
     3241 atctgccacg acggaaaggc tcacttcccc agggagggag tgttcgtgtc taacggaacc
     3301 cactggttcg tgacccagag gaacttctac gagccacaga tcatcaccac cgacaacacc
     3361 ttcgtgtctg gaaactgcga cgtggtgatc ggaatcgtga acaacaccgt gtacgaccca
     3421 ctgcagccag aactggactc tttcaaggag gaactggaca agtacttcaa gaaccacacc
     3481 tctccagacg tggacctggg agacatctcc ggaatcaacg cctctgtggt gaacatccag
     3541 aaggaaatcg acagactgaa cgaggtggcc aagaacctga acgaatctct gatcgacctg
     3601 caggaactgg gaaagtacga gcagtacatc aagtggccc
//
